Systematic Analysis of Tobacco CrRLK1L Family Genes and Functional Identification of NtCrRLK1L47 in Environmental Stresses

Li, Xiaoxu; Guo, Chunfang; Wang, Qi; Li, Zhiyuan; Cai, Jun; Wu, Dousheng; Li, Yangyang; Yang, Aiguo; Guo, Yongfeng; Gao, Junping; Wen, Liuying; Pu, Wenxuan

doi:10.3389/fpls.2022.838857

Cited by 8 publications

(12 citation statements)

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“…It has been experimentally demonstrated that the CrRLK1L genes have tissue-specific expression patterns in Arabidopsis , tobacco, and apple [ 34 , 39 , 40 ]. To study the tissue-specific expression in tomato CrRLK1L genes, the expression profiles of all of the 24 SlCrRLK1L genes were examined in ten samples (root, leaf, bud, flower, from 1 cm to 3 cm of fruit, mature green fruit, breaker fruit, and breaker plus a 10-day fruit).…”

Section: Resultsmentioning

confidence: 99%

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Genome-Wide Re-Identification and Analysis of CrRLK1Ls in Tomato

Liu

Chen

et al. 2023

IJMS

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The Catharanthus roseus receptor-like kinase 1-like (CrRLK1L), which is a vital member of the plant receptor-like kinase family, plays versatile roles in plant growth, development, and stress response. Although the primary screening of tomato CrRLK1Ls has been reported previously, our knowledge of these proteins is still scarce. Using the latest genomic data annotations, a genome-wide re-identification and analysis of the CrRLK1Ls in tomatoes were conducted. In this study, 24 CrRLK1L members were identified in tomatoes and researched further. Subsequent gene structures, protein domains, Western blot analyses, and subcellular localization analyses all confirmed the accuracy of the newly identified SlCrRLK1L members. Phylogenetic analyses showed that the identified SlCrRLK1L proteins had homologs in Arabidopsis. Evolutionary analysis indicated that two pairs of the SlCrRLK1L genes had predicted segmental duplication events. Expression profiling analyses demonstrated that the SlCrRLK1L genes were expressed in various tissues, and most of them were up- or down-regulated by bacteria and PAMP treatments. Together, these results will lay the foundation for elaborating the biological roles of SlCrRLK1Ls in tomato growth, development, and stress response.

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Section: Resultsmentioning

confidence: 99%

“…In pears ( Pyrus bretchneideri ), PbrCrRLK1L3 and PbrCrRLK1L26 take part in the pollen tube rupture process and growth [ 32 ]. Additionally, Chenopodium quinoa CqFER, soybean GmCrRLK1L20, and tobacco NtCrRLK1L47 have been reported to be involved in salt stress responses [ 33 , 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Re-Identification and Analysis of CrRLK1Ls in Tomato

Liu

Chen

et al. 2023

IJMS

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“…As a result, it is difficult to explain why OsRALF26-mediated ROS burst was not significantly observed in tobacco, unlike in Arabidopsis. A genome-wide analysis of tobacco identified 48 CrRLK1L genes (Li, Guo, et al, 2022), and some of the CrRLK1Ls should function as tobacco FLRs and recognize tobacco RALFs. Therefore, it is possible that the structural difference between OsRALF26 and its tobacco homologs may (a) Disease symptom on Arabidopsis Col-0 and transgenic Col-0 overexpressing OsRALF26-GFP (OsRALF26-GFPox/Col-0) (T 2 ; progeny of lines 2 and 7) after Pst DC3000 inoculation (upper panel).…”

Section: Discussionmentioning

confidence: 99%

Rice small secreted peptide, OsRALF26, recognized by FERONIA‐like receptor 1 induces immunity in rice and Arabidopsis

Kwon,

Moon,

Jeong

et al. 2024

The Plant Journal

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SUMMARYRapid alkalinization factors (RALFs), belonging to a family of small secreted peptides, have been considered as important signaling molecules in diverse biological processes, including immunity. Current studies on RALF‐modulated immunity mainly focus on Arabidopsis, but little is reported in crop plants. The rice immune receptor XA21 confers immunity to the bacterial blight pathogen, Xanthomonas oryzae pv. oryzae (Xoo). Here, we pursued functional characterization of rice RALF26 (OsRALF26) up‐regulated by Xoo during XA21‐mediated immune response. When applied exogenously as a recombinant peptide, OsRALF26 induced a series of immune responses, including pathogenesis‐related genes (PRs) induction, reactive oxygen species (ROS) production, and callose deposition in rice and/or Arabidopsis. Transgenic rice and Arabidopsis overexpressing OsRALF26 exhibited significantly enhanced resistance to Xoo and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), respectively. In yeast two‐hybrid, pull‐down assays, and co‐immunoprecipitation analyses, rice FER‐like receptor 1 (OsFLR1) was identified as a receptor of OsRALF26. Transient expression of OsFLR1 in Nicotiana benthamiana leaves displayed significantly increased ROS production and callose deposition after OsRALF26 treatment. Together, we propose that OsRALF26 induced by Xoo in an XA21‐dependent manner is perceived by OsFLR1 and may play a novel role in the enforcement of XA21‐mediated immunity.

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“…Bs4C was synthesized by Sangon Biotech. Both were assembled into pCHF3 and transformed into N. tabacum K326 using A. tumefaciens LBA4404 as previously described (Li et al ., 2022 ). T0 plants were screened on MS medium containing 50 mg/L kanamycin and genotyped with transgene‐specific primers.…”

Section: Methodsmentioning

confidence: 99%

An ancient cis‐element targeted by Ralstonia solanacearum TALE‐like effectors facilitates the development of a promoter trap that could confer broad‐spectrum wilt resistance

Gallas,

Li,

von Roepenack‐Lahaye

et al. 2023

Plant Biotechnology Journal

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SummaryRalstonia solanacearum, a species complex of bacterial plant pathogens that causes bacterial wilt, comprises four phylotypes that evolved when a founder population was split during the continental drift ~180 million years ago. Each phylotype contains strains with RipTAL proteins structurally related to transcription activator‐like (TAL) effectors from the bacterial pathogen Xanthomonas. RipTALs have evolved in geographically separated phylotypes and therefore differ in sequence and potentially functionality. Earlier work has shown that phylotype I RipTAL Brg11 targets a 17‐nucleotide effector binding element (EBE) and transcriptionally activates the downstream arginine decarboxylase (ADC) gene. The predicted DNA binding preferences of Brg11 and RipTALs from other phylotypes are similar, suggesting that most, if not all, RipTALs target the Brg11‐EBE motif and activate downstream ADC genes. Here we show that not only phylotype I RipTAL Brg11 but also RipTALs from other phylotypes activate host genes when preceded by the Brg11‐EBE motif. Furthermore, we show that Brg11 and RipTALs from other phylotypes induce the same quantitative changes of ADC‐dependent plant metabolites, suggesting that most, if not all, RipTALs induce functionally equivalent changes in host cells. Finally, we report transgenic tobacco lines in which the RipTAL‐binding motif Brg11‐EBE mediates RipTAL‐dependent transcription of the executor‐type resistance (R) gene Bs4C from pepper, thereby conferring resistance to RipTAL‐delivering R. solanacearum strains. Our results suggest that cell death‐inducing executor‐type R genes, preceded by the RipTAL‐binding motif Brg11‐EBE, could be used to genetically engineer broad‐spectrum bacterial wilt resistance in crop plants without any apparent fitness penalty.

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Systematic Analysis of Tobacco CrRLK1L Family Genes and Functional Identification of NtCrRLK1L47 in Environmental Stresses

Cited by 8 publications

References 59 publications

Genome-Wide Re-Identification and Analysis of CrRLK1Ls in Tomato

Genome-Wide Re-Identification and Analysis of CrRLK1Ls in Tomato

Rice small secreted peptide, OsRALF26, recognized by FERONIA‐like receptor 1 induces immunity in rice and Arabidopsis

An ancient cis‐element targeted by Ralstonia solanacearum TALE‐like effectors facilitates the development of a promoter trap that could confer broad‐spectrum wilt resistance

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