Systematic characterization of chemical constituents in Mahuang decoction by UHPLC tandem linear ion trap‐Orbitrap mass spectrometry coupled with feature‐based molecular networking

Ling, Chen; Yao, Changliang; Li, Jiayuan; Wang, Jing; Shuai, Yao; Shen, Shijie; Yang, Lin; Wei, Wenlong; Bi, Qirui; Guo, Dan

doi:10.1002/jssc.202100121

Cited by 18 publications

(15 citation statements)

References 26 publications

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“…21 MHD, a well-known traditional Chinese medicine prescription, has been extensively applied for relieving the cold, influenza, cough, acute bronchitis, asthma and other pulmonary diseases. 22,23 Here, this study suggested that MHD could potentially decrease SP1 expression and disrupted FGFR3-dependent PI3K/AKT signaling pathway activation, consequently preventing airway inflammation and remodeling, therefore delaying asthma progression (Figure 8).…”

Section: Discussionmentioning

confidence: 80%

Mahuang Decoction Attenuates Airway Inflammation and Remodeling in Asthma via Suppression of the SP1/FGFR3/PI3K/AKT Axis

Wei¹,

Gou²,

Su³

et al. 2022

DDDT

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Background/Purpose Mahuang decoction (MHD) is a classic famous traditional Chinese medicine and has various pharmacological effects, including anti-inflammation and anti-asthma. In this study, we aimed to investigate the potential protective effect of MHD against asthma and elucidated the underlying mechanism. Materials and Methods A mouse model of asthma was induced by ovalbumin (OVA) treatment, and then treated with MHD to evaluate its effect on the asthma. Gain- or loss-of-function approaches were performed in SP1 and FGFR3 to study their roles in asthma via measurement of airway inflammation, airway remodeling and airway smooth muscle cell (ASMC) proliferation-related factors. Results MHD reduced airway inflammation and remodeling. Additionally, MHD contributed to diminished expression of SP1, which was shown to repress airway inflammation and remodeling. Furthermore, SP1 bound to the FGFR3 promoter, resulting in the FGFR3 transcription promotion and ASMC proliferation. Conversely, FGFR3 knockdown abolished airway inflammation and remodeling, the mechanism of which was related to suppression of the PI3K/AKT signaling pathway. Meanwhile, MHD hindered airway inflammation and remodeling following asthma by suppressing the SP1/FGFR3/PI3K/AKT axis. Conclusion Taken together, MHD may retard airway inflammation and remodeling by suppressing the SP1/FGFR3/PI3K/AKT axis, which contributes to an extensive understanding of asthma and may provide novel therapeutic options for this disease.

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Section: Discussionmentioning

confidence: 80%

Mahuang Decoction Attenuates Airway Inflammation and Remodeling in Asthma via Suppression of the SP1/FGFR3/PI3K/AKT Axis

Wei¹,

Gou²,

Su³

et al. 2022

DDDT

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“…The most important feature of molecular networks is the ability to visualize groups of components in SJZD, complementing traditional chemical analysis. Compounds with similar structures have similar fragmentation spectra and their nodes are often clustered together to form clusters of analogs [39]. Molecular networks can provide a fast and sensitive way to simultaneously analyze the chemical composition of a plant extract, effectively saving human time and effort.…”

Section: Resultsmentioning

confidence: 99%

Multiple approaches to characterize and visualize the chemical composition of Sijunzi Decoction comprehensively

Chen

Dong

et al. 2023

J of Separation Science

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and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine. Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines. Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids, and inorganic elements in the decoction were analyzed by multiple approaches. A molecular network was also used for visualizing the ingredients in Sijunzi Decoction, and representative components were also quantified. The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids, and 0.483% inorganic elements. Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction. The present study systematically characterized the constituents of Sijunzi Decoction, revealed the composition ratio of each type of constituent, and provided a reference for study on the substance basis of other Chinese medicine.

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“…F'7 ( t R = 9.23 min) showed [M‐H] – ions at m/z 479.1193 (calculated for C 22 H 22 O 12 ). The fragment ions at m/z 303 in the MS 2 spectra were generated by GluA and CH 3 loss in succession from ions at m/z 479 [19]. Thus, F'7 was determined to be a methylation metabolite of flavanomarein.…”

Section: Resultsmentioning

confidence: 99%

Study on the metabolism profile of flavanomarein in Coreopsis tinctoria Nutt

Shi

Tang

Xing

et al. 2022

J of Separation Science

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Coreopsis tinctoria Nutt. (family Asteraceae) is a popular medicine-food plant, which improves chronic diseases such as hyperlipemia, hypertension, and diabetes. Flavanomarein is the main active component of Coreopsis tinctoria Nutt, in which the blood concentration of volunteers is low and bioavailability is poor.Thus, the understanding of flavanomarein metabolites and metabolic pathways is significant to clarify its effectiveness. This study systematically studied the metabolites of flavanomarein by oral and injection. The biological samples (feces, urine, and plasma) were analyzed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry in negative ion mode. The metabolic law of flavanomarein in the liver was further verified by a liver microsomal incubation experiment in vitro. A total of 12 metabolites were identified by oral administration while 15 metabolites were detected by injection. It was shown that metabolic pathways include acetylation, hydroxylation, glucuronidation, methylation, dehydrogenation, and so forth. The liver extraction rate of flavanomarein was 0.08, which means the metabolic stability of flavanomarein is well in rats' liver microsomes. It is a systematic study on the metabolism of flavanomarein and provides a metabolic rationale for further in-depth in vivo biotransformation.

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Systematic characterization of chemical constituents in Mahuang decoction by UHPLC tandem linear ion trap‐Orbitrap mass spectrometry coupled with feature‐based molecular networking

Cited by 18 publications

References 26 publications

Mahuang Decoction Attenuates Airway Inflammation and Remodeling in Asthma via Suppression of the SP1/FGFR3/PI3K/AKT Axis

Mahuang Decoction Attenuates Airway Inflammation and Remodeling in Asthma via Suppression of the SP1/FGFR3/PI3K/AKT Axis

Multiple approaches to characterize and visualize the chemical composition of Sijunzi Decoction comprehensively

Study on the metabolism profile of flavanomarein in Coreopsis tinctoria Nutt

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