Systematic characterization of human CD8⁺ T cells with natural killer cell markers in comparison with natural killer cells and normal CD8⁺ T cells

Abstract

SUMMARY+ T cells>CD57 + T cellsiCD8 + T cellsiNK cells. However, the CD3-stimulated proliferation of both NK-type T cells was smaller than that of CD8 + T cells partly because NK-type T cells were susceptible to apoptosis. In addition to NK cells, NK-type T cells but not CD8+ T cells stimulated with cytokines, expressed cytoplasmic perforin and granzyme B. Furthermore, CD3-stimulated IFN-c production from peripheral blood mononuclear cells (PBMC) correlated with the proportions of CD57 + T cells in PBMC from donors. Our ®ndings suggest that NK-type T cells play an important role in the T helper 1 responses and the immunological changes associated with ageing.

Activation-Induced Expression of CD56 by T Cells Is Associated With a Reprogramming of Cytolytic Activity and Cytokine Secretion Profile In Vitro

“…ϩ T cells [3,5], CD56 ϩ T cells were found to release higher levels of the Th1 cytokine, IFN-␥ than did similar numbers of CD56 Ϫ T cells. We found that the kinetics of IFN-␥ release were similar for both lymphocyte subsets, with maximal levels detectable after 48 hours.…”

“…ϩ T cells belong to a heterogenous group of human T cells that express various stimulatory, costimulatory, inhibitory, and adhesion receptors that control NK cell activity [3][4][5][33][34][35]. T cells with NKRs, including CD56 ϩ T cells, are often present in cultures of stimulated T cells [4,11,[13][14][15][16][17], and they frequently express memory phenotypes [2,36,37].…”

“…CD56 ϩ T cells are heterogenous in nature and include CD4 ϩ , CD8 ϩ , and CD4 Ϫ CD8 Ϫ cells expressing ␣␤ or ␥␦ major histocompatibility complex (MHC) or CD1d-restricted T-cell receptors (TCRs), and various combinations of NK cell receptors (NKRs), including CD16, CD56, CD94/NKG2, CD161, NKG2D, and killer immunoglobulinlike receptors [3][4][5]. They generally account for less that 5% of peripheral blood T cells and up to 50% of T cells in the liver [6,7] and in the intestine [8 -10].…”

Persistent accumulation of interferon-γ-producing CD8+CD56+ T cells in blood from patients with coronary artery disease

“…In vitro, CD56 + T cells have shown the capacity to produce both type 1 and type 2 cytokines 10,[15][16][20][21] .…”

Refining the diagnosis of T-cell large granular lymphocytic leukemia by combining distinct patterns of antigen expression with T-cell clonality studies

“…Some studies have defined CD8 þ T cells with expression of CD57 as replicatively scenescent T cells, 19 whereas others demonstrate these cells to be effector cytotoxic T cells that are increased in infections or neoplasms and represent a chronically activated state. [20][21][22][23][24] Still, others have shown that expansions of CD8 þ /CD57 þ T cells are seen with increased frequency in the elderly, and they have postulated defects in immune system homeostasis leading to expansion of these T cells and diminished immune system surveillance. 25 Nonetheless, we are not aware of significant studies on T cells with the phenotype of CD8 þ (dim)/CD57 þ expression.…”