Systematic error and comparison of four methods for assessing the viability ofSaccharomyces cerevisiae suspensions

Lange, Hélène; Bavouzet, Jean-Michel; Taillandier, Patricia; Delorme, Caroline

doi:10.1007/bf02566152

Cited by 44 publications

(30 citation statements)

References 8 publications

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“…Similar tests showed a non-signi®cant 2% drop of E coli viability (compared with the experimental error of the method used for the determination of viability 14 ). Neither lysis nor morphological change occurred during this preliminary experiment.…”

Section: Validation Of the Experimental Set-upmentioning

confidence: 60%

Effect of high shear stress on microbial viability

Lange

Taillandier

Riba

2001

J of Chemical Tech & Biotech

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Escherichia coli and Saccharomyces cerevisiae suspensions were submitted to controlled shear stress. Above a threshold value shear stress induced a decrease in micro-organism viability. The threshold of shear stress ef®ciency depended on the micro-organisms, being between 1292 Pa and 2770 Pa for S cerevisiae, and about 1250 Pa for E coli. Above 1810 Pa, E coli cells were disrupted whereas the S cerevisiae cells remained intact. The higher the cellular concentration, the greater the rate of decrease in viability. Viability loss was in¯uenced by the number of passages through the experimental shear stress device and by exposure time.

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Section: Validation Of the Experimental Set-upmentioning

confidence: 60%

Effect of high shear stress on microbial viability

Lange

Taillandier

Riba

2001

J of Chemical Tech & Biotech

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“…Cell viability was assessed following the methylene blue staining method proposed by Lange et al (17). For evaluation of substrates and products, samples were centrifuged for 10 min at 9,391×g (Centrifuge 5424; Eppendorf, Hamburg, Germany) and concentrations were determined based on HPLC (600 TSP Spectra System; Waters Corporation, Milford, MA, USA) using a Shodex SH1011 column (8×300 mm) maintained at 55 o C with a mobile phase of 0.05 N sulfuric acid at a flow rate of 0.6 mL/min using an Index Refraction Detector (2414 TSP Refracto Monitor; Waters Corporation).…”

Section: Methodsmentioning

confidence: 99%

Effects of oxygen and nutrients on xylitol and ethanol production in sugarcane bagasse hydrolyzates

Castañón-Rodríguez

Portilla-Arias

Aguilar-Uscanga

et al. 2015

Food Sci Biotechnol

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The influence of oxygen and nutrient supplementation on xylitol and ethanol production in a synthetic medium and sugarcane bagasse hydrolyzates using Candida tropicalis IEC5-ITV and Saccharomyces cerevisiae ITV01-RD was investigated for evaluation of bioconversion of pentoses and hexoses present in the lignocellulosic biomass. The best oxygen transfer rate (56.05 mg of O 2 /L/h) and xylitol and ethanol yields (0.67 and 0.47 g/g, respectively) were obtained in a synthetic medium. A yeast extract had a positive effect on xylitol and ethanol production (0.64 and 0.44 g/g, respectively) at a concentration of 1 g/L. C. tropicalis and S. cerevisiae exhibited not previously observed morphological changes depending on the nutrient composition. Use of sugarcane bagasse hydrolyzates requires a good supply of oxygen and addition of a yeast extract to improve xylitol and ethanol production.

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“…Viability was obtained by the methylene blue staining method [17]. Additionally, the culture medium was centrifuged for 10 min at 10,000 rpm (Eppendorf Centrifuge 5424, Germany) and the supernatant stored at -20°C until analysis.…”

Section: Analytical Techniquesmentioning

confidence: 99%

Ethanol production by Pichia stipitis immobilized on sugarcane bagasse

Yñiguez-Balderas¹,

Benigno²,

Gómez-Rodríguez³

et al. 2016

Bioethanol

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The search for new ethanol production technologies is due to this biofuel being a renewable and environmentally friendly option. Immobilized cell systems for ethanol production have been studied; however, the phenomenon involved in cell sorption on raw materials has been poorly explored. Therefore, this work evaluates P. stipitis immobilization on sugarcane bagasse pretreated with sulphuric acid, as well as ethanol production in batch culture. The results obtained showed that the Guggenheim-Anderson-de Boer (GAB) model explained the sorption phenomenon. The selected inoculum size for immobilization was the same as the monolayer sorption capability (1.17 gl-1). Using 1:100 g ml- 1 solid-liquid ratio, at 250 rpm, ethanol yield and productivity of 0.404 gg-1 glucose and 0.41 gl-1h-1 were obtained, respectively. The immobilized systems were stable for up to twenty-five repeated batches (36 h each). Ethanol production was increased from the first to the twenty-fifth batch (18.1 and 24.7 gl-1 ethanol). The use of complex media, such as molasses “B” or sugarcane hydrolyzates, caused an increase in process efficiency 2.4 and 1.8-fold respectively, compared with free cells systems. Biotechnological ethanol production from lignocellulosic hydrolyzates could be improved by the use of the immobilization cell sorption on pre-treated raw materials.

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Systematic error and comparison of four methods for assessing the viability ofSaccharomyces cerevisiae suspensions

Cited by 44 publications

References 8 publications

Effect of high shear stress on microbial viability

Effect of high shear stress on microbial viability

Effects of oxygen and nutrients on xylitol and ethanol production in sugarcane bagasse hydrolyzates

Ethanol production by Pichia stipitis immobilized on sugarcane bagasse

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