Systematic identification and characterization of differentially expressed microRNAs under tetraniliprole exposure in the fall armyworm, Spodoptera frugiperda

Abstract

The fall armyworm, Spodoptera frugiperda, is a worldwide agricultural pest and causes huge losses of crop production each year. Tetraniliprole is a novel diamide insecticide with high efficacy against even the insecticide resistant pests of Lepidoptera, Coleoptera, and Diptera. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level and play an important regulatory role in the insecticide resistance in insects. However, the effects of miRNAs on the tetraniliprole tolerance in S. frugiperda are poorly understood. In the present research, the miRNAs response to tetraniliprole application in S. frugiperda were systematically investigated by high‐throughput sequencing. A total of thirty differentially expressed miRNAs were identified under tetraniliprole treatment in S. frugiperda. The functions of the target genes of these differentially expressed miRNAs were further predicted by Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes database pathway, and the most significantly enriched pathway was MAPK signaling pathway. The expression changes of six differentially expressed miRNAs were validated by quantitative real‐time polymerase chain reaction. Furthermore, miR‐278‐5p had the highest expression in the hemolymph and malpighian tubule and the lowest expression in the gut. Oversupply of miR‐278‐5p significantly increased the mortality of S. frugiperda following exposure to tetraniliprole. These results will provide the basis for understanding the regulatory roles of miRNAs regarding to tetraniliprole tolerance in S. frugiperda.

Cyantraniliprole and Thiamethoxam Exposure Changes Expression of Transcripts Associated with Small Non-Coding RNA Processing in the Colorado Potato Beetle

“…The miRNAs miR-7a and miR-8519 have been shown to regulate the expression of PxRyR , a key target associated with chlorantraniliprole susceptibility in the diamondback moth, Plutella xylostella [ 26 ]. Expression profiling of miRNAs in fall armyworms, Spodoptera frugiperda , subjected to tetraniliprole was associated with varied levels of 30 miRNAs, and subsequent miR-278-5p modulation could impact the response to this compound in this agricultural pest [ 27 ]. Results from the current study highlight a modulation of Ago1 transcript levels in adult L. decemlineata exposed to the diamide cyantraniliprole.…”

miRNA Dynamics for Pest Management: Implications in Insecticide Resistance

“…Furthermore, in silico prediction is extensively utilized to identify potential targets linked to these differentially expressed miRNAs. This involves investigating their roles in metabolic pathways, detoxification processes, and other cellular mechanisms related to resistance [42].…”

“…RNA (including miRNA) is extracted from the collected samples to quantify the expression patterns of these differentially expressed miRNAs and their predicted target genes in response to insecticide exposure compared to untreated control insects using RT-qPCR. Additionally, specific tissues (like the head, gut, cuticle, malpighian tubule, or fat body) may be targeted for analyzing miRNA and gene expression to study the spatiotemporal dynamics and miRNA functions in insects [42]. Overall, the results might indicate that miRNAs modulate resistance to insecticides by targeting genes that are presumed to be involved in the detoxification of insecticides [40][41][42][43].…”

Construction of a nontoxic nano-pesticide and its co-application with natural predators for perfect cooperative pest management: an innovative strategy for pesticide reduction

“…Tetraniliprole (TTP) is a bisamide insecticide, primarily targeting insect ryanodine receptors and exhibiting high insecticidal activity against lepidopteran pests. 30,31 The predatory stinkbug Arma custos (Hemiptera: pentatomidae) is a broad-spectrum predator that is widely distributed across numerous provinces in China. 32 It possesses excellent control capability against various agricultural and forestry pests in the orders of Lepidoptera, Hemiptera, and Coleoptera.…”