Systematic identification metabolites of Hemerocallis citrina Borani by high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening method

Liu, Jinghong; Zhong, Xiaohong; Jiang, Yingying; Yu, Liuyi; Huang, Xiuqiong; Dong, Zhuang; Yang, Shuaiyong; He, Wei; Zeng, Jianguo; Qing, Zhixing

doi:10.1016/j.jpba.2020.113314

Cited by 35 publications

(14 citation statements)

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“…The HCW and HCE of H. citrina was preliminarily analyzed by HPLC-Q-TOF-MS to find the main ingredients contributing to the antidepressant activity. A total of 32 high-level metabolites, including 18 flavonoids, 7 chlorogenic acid-type, 3 polyphenols, 2 acetamide alkaloids and 2 diterpenoid saponins, were screened and tentatively identified by their tandem mass spectrometry (MS/MS) [( Table 1 and Figure 3 ) ( Liu et al, 2020 )]. And then, the high-content ingredient (compound 15 ) was further isolated by the MS-guided isolation method ( Qing et al, 2014 ; Qing et al, 2016 ; Qing et al, 2017b ), and its structure was unambiguously identified by nuclear magnetic resonance (NMR) data.…”

Section: Resultsmentioning

confidence: 99%

“…The difference m/z value of compounds 6 and 7 was 15.9942 Da, which indicated that one of the hydroxyl groups in compound 7 was replaced by a hydrogen atom and formed the structure of 6 . In the MS/MS spectrum of compound 6 ( Figure 4B ), the high abundance of ions at m/z 163.0379 and 119.0463 were formed, which demonstrated that one of the hydroxyl groups connected to the benzene ring was replaced, therefore, compound 6 was tentatively identified as 5- O -p-coumaroylquinic acid ( Liu et al, 2020 ). In addition, metabolites 9 , 21 , and 30 were unambiguously identified as caffeic acid, rutin, and quercetin, respectively, by comparing the retention time, MS and MS/MS data with the standards.…”

Section: Resultsmentioning

confidence: 99%

“…H. citrina Baroni (It was also called “Huang Hua Cai” in Chinese) has been widely grown in China, Japan, and Korea, and its flower buds are one of the most commonly consumed vegetables in Asia ( Ma et al, 2018 ; Liu et al, 2020 ). The flower buds of H. citrina have been recorded to relieve depression in the medicinal book “Compendium of Materia Medica”, which is a famous Chinese encyclopedia of medicine written by Li et al (2017) in the Ming dynasty ( Xu et al, 2020 ; Qing et al, 2021a ).…”

Section: Introductionmentioning

confidence: 99%

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Antidepressant-like activity, active components and related mechanism of Hemerocallis citrina Baroni extracts

et al. 2022

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Hemerocallis citrina Baroni [Asphodelaceae], which is traditional herbal medicine, has been widely used for treating depressive disorders in Eastern-Asia countries. However, the active compounds and corresponding mechanism of anti-depression are not yet completely clarified. In this study, the anti-depressive activities of six H. citrina extracts were primarily evaluated. The results showed that the water extract of H. citrina flowers (HCW) displays significant anti-depressive activity. A total of 32 metabolites were identified from HCW by high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) and nuclear magnetic resonance (NMR). And then, the anti-depressive activity of the high-level compound (rutin) in HCW was also estimated. The results indicated that rutin displayed significant anti-depressive activity and was one of the main active ingredients. Finally, the anti-depressive mechanisms of HCW and rutin were investigated based on the intestinal microorganisms. The results showed that HCW and rutin increase the diversity and richness of the intestinal flora and regulate the specific intestinal microorganisms such as Bacteroides and Desulfovibrio genera in depressed mice. This work marks the first comprehensive study of the active components, anti-depressive activities and corresponding mechanisms of different H. citrina extracts, which provide a potential possibility for developing new antidepressants.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antidepressant-like activity, active components and related mechanism of Hemerocallis citrina Baroni extracts

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“…Hemerocallis citrina Borani is rich in nutrition, which is a typical health vegetable with rich in protein, vitamins, and minerals. H. citrina Borani has been demonstrated multiple functions including antioxidant ( 2 ), antidepressant ( 3 , 4 ), lactation improving ( 5 ), and sleep promoting ( 6 ), as documented in the medicinal book “Compendium of Materia Medica” ( 7 ). The industry of H. citrina Borani is so far in the state of primary processing, with poor economic benefits and low value-added.…”

Section: Introductionmentioning

confidence: 99%

Polysaccharide from Hemerocallis citrina Borani by subcritical water with different temperatures and investigation of its physicochemical properties and antioxidant activity

Ban

Wang

et al. 2022

Front. Nutr.

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Hemerocallis citrina Borani as a low-cost vegetable, has various health benefits. However, the industry of H. citrina Borani is in the state of primary processing, with poor economic benefits. This study aimed to investigate the physicochemical properties, and the antioxidant activity of H. citrina Borani polysaccharide (HCBP) using subcritical water extraction (SWE) at different temperatures, to expand the value of H. citrina Borani. HCBP mainly composed of nine monosaccharides (glucose, galactose, rhamnose, fucose, mannose, arabinose, xylose, galacturonic acid, and glucuronic acid), among which the content of neutral sugar was higher and uronic acid was lower. HCBP contained glycosidic bond of β-configurations and trace quantities protein. The molecular weight of HCBP decreased with increasing temperature. Shear thinning occurred in HCBP with the increase of shear rate (0.01–1 s−1), and the apparent viscosity of HCBP decreased at higher temperature (150–160°C) with the increase continuously of shear rate (1–10 s−1), but almost remained constant at lower temperature (130–140°C). Scanning electron microscope showed that HCBP had rough surface, loose structure, obvious particle gap, and irregular shape. In addition, HCBP extracted at 160°C had strong FRAP activity, and HCBP extracted at 130 and 140°C had better ABTS radical scavenging activity. This study suggests that HCBP extracted by SWE could provide a cheap raw material as food thickening agent and natural antioxidants.

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“…Misuse, overdose, poisoning, and suicide caused by colchicine occur occasionally (Finkelstein et al, 2010; Schreiber et al, 2019). In addition, colchicine poisoning can result from the consumption of fresh Huang Hua Cai, which is the flower bud of Hemerocallis citrina Baroni and is edible in some areas of China after special treatment (Liu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Development, validation, and clinical application of a rapid UPLC–MS/MS method for detection of colchicine in human whole blood and urine

Gao

et al. 2022

Biomedical Chromatography

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A rapid, simple, and economical method has been developed to determine colchicine in both human whole blood and urine using UPLC-MS/MS. Colchicine and isotopelabeled internal standard were extracted from the matrix by liquid-liquid extraction with saturated borax and ethyl acetate, and separated by a reversed-phase chromatography C18 column. Gradient elution was carried out using acetonitrile and water spiked with 0.01% formic acid. Multiple reaction monitoring was performed at positive ion mode. The quantitative transitions were m/z 400.27 ! 310.28 for colchicine and m/z 406.16 ! 313.18 for colchicine-D6. The method has good linearity in the range of 0.5-200 ng/mL for blood and 2-2000 ng/mL for urine. The sensitivity, accuracy, and matrix effect were all in line with the guidelines of Food and Drug Administration and European Medicines Agency. The extraction recovery was above 63.94%. The samples were stable under various storage conditions. Six deuteriumsubstituted isotopic internal standard was used to demonstrate a different mode of colchicine cleavage from the existing literature. This method has been successfully used in the diagnosis of patients with colchicine poisoning. Blood is recommended as the optimal sample compared with urine.

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Systematic identification metabolites of Hemerocallis citrina Borani by high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry combined with a screening method

Cited by 35 publications

References 28 publications

Antidepressant-like activity, active components and related mechanism of Hemerocallis citrina Baroni extracts

Antidepressant-like activity, active components and related mechanism of Hemerocallis citrina Baroni extracts

Polysaccharide from Hemerocallis citrina Borani by subcritical water with different temperatures and investigation of its physicochemical properties and antioxidant activity

Development, validation, and clinical application of a rapid UPLC–MS/MS method for detection of colchicine in human whole blood and urine

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