Systematic identification of chemical components in Fufang Shuanghua oral liquid and screening of potential active components against SARS-CoV-2 protease

Jiang, Hong; Chen, Jie; Li, Xin; Zhong, Yute; Kang, Liping; Wang, Guohua; Yu, Meng; Liu, Fu; Wang, Ping; Xu, Haiyu

doi:10.1016/j.jpba.2022.115118

Cited by 10 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antioxidant and anti-in ammatory properties of forsythoside A may help quench reactive oxygen species and regulate the immune function, and thereby ameliorate the symptoms of COVID-19. In addition, forsythoside A was found to directly act on SARS-CoV-2 main protease through CYS145 and HIS41 sites, which may inhibit the SARS-CoV-2 replication and transcription [20]. was identi ed as forsythialan B. Forsythialan B is a tetrahydrofuran, one of the representative lignans in Lianqiao, which has been proven to be protective against peroxynitrite-induced oxidative stress [21].…”

Section: Resultsmentioning

confidence: 99%

Phytochemicals Characterization of Lianqiao (Forsythia suspensa) extract and evaluation of its antivirus and antioxidant activities

Gao

Zhu

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

Forsythia suspensa (Lianqiao) has long been touted for its anti-viral effects in folklore. Although promising, limited research were conducted to evaluate its potential in defending the severe acute respiratory syndrome coronavirus (SARS-CoV-2). This study evaluated the fruits of Forsythia suspensa (Lianqiao), an important economic crop, for the chemical components of its water and ethanol extracts, along with their inhibitory effects on SARS-CoV-2 spike protein binding to angiotensin-converting enzyme 2 (ACE2), inhibitions of ACE2 activity, and capacities to scavenge the selected free radicals. A total of 42 compounds were tentatively identified in the extracts by HPLC-MS/MS analysis. Both water and ethanol extracts of Lianqiao showed great inhibition of ACE2 activity and the binding affinity between SARS CoV-2 spike protein and ACE2. Furthermore, the water extract had greater scavenging capacities against HO●, DPPH● and ABTS●+ than the ethanol extract. Findings of this research contribute to the knowledge of different extraction solvent systems and support the potential of Lianqiao in reducing the risk of coronavirus infection and alleviating the symptoms after infection.

show abstract

Section: Resultsmentioning

confidence: 99%

Phytochemicals Characterization of Lianqiao (Forsythia suspensa) extract and evaluation of its antivirus and antioxidant activities

Gao

Zhu

Liu

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Atmospheric pressure chemical ionization (APCI) MS shows peaks at m/z 354.9 and 163.1 [38]. A chemical diagram of ion 163 _b is presented in [40], while that of ion 163 _c is presented in [43]. The fragmentation pattern within m/z = 0-100 is assigned in [40,41,43].…”

Section: Mass Spectrometric Data On Chlorogenic Acidmentioning

confidence: 99%

“…Figure 4 depicts fragmentation reactions of chlorogenic acid. One might argue that its MS fragmentation processes have been extensively examined [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52]. Thus, we only sketch out MS analysis of CA showing the intensity ratios of the peaks at m/z 191 and 179, thus quantitatively distinguishing not only among its positional 1-O-, 3-O-, 4-O-, and 5-Oisomers, but also between transand cis-isomers of the product 3-(3,4-dihydroxy-phenyl)acrylic acid anions [35].…”

Section: Mass Spectrometric Data On Chlorogenic Acidmentioning

confidence: 99%

See 1 more Smart Citation

Mass Spectrometric Identification of Metabolites after Magnetic-Pulse Treatment of Infected Pyrus communis L. Microplants

Upadyshev,

Ivanova,

Motyleva

2023

IJMS

View full text Add to dashboard Cite

The major goal of this study is to create a venue for further work on the effect of pulsed magnetic fields on plant metabolism. It deals with metabolite synthesis in the aforementioned conditions in microplants of Pyrus communis L. So far, there have been glimpses into the governing factors of plant biochemistry in vivo, and low-frequency pulsed magnestatic fields have been shown to induce additional electric currents in plant tissues, thus perturbing the value of cell membrane potential and causing the biosynthesis of new metabolites. In this study, sixty-seven metabolites synthesized in microplants within 3–72 h after treatment were identified and annotated. In total, thirty-one metabolites were produced. Magnetic-pulse treatment caused an 8.75-fold increase in the concentration of chlorogenic acid (RT = 8.33 ± 0.0197 min) in tissues and the perturbation of phenolic composition. Aucubin, which has antiviral and antistress biological activity, was identified as well. This study sheds light on the effect of magnetic fields on the biochemistry of low-molecular-weight metabolites of pear plants in vitro, thus providing in-depth metabolite analysis under optimized synthetic conditions. This study utilized high-resolution gas chromatography-mass spectrometry, metabolomics methods, stochastic dynamics mass spectrometry, quantum chemistry, and chemometrics, respectively. Stochastic dynamics uses the relationships between measurands and molecular structures of silylated carbohydrates, showing virtually identical mass spectra and comparable chemometrics parameters.

show abstract

“…The main protease (Mpro) of SARS-CoV-2 is an important non-structural protein mediates viral replication and transcription. It was found that the Mpro enzymatic activity was inhibited at IC 50 =22.26 μM of FTA and the docking results indicated that FTA was able to bind some sites of Mpro [8] . What's more, FTA also had a direct virucidal effect on IBV [9] .…”

Section: Introductionmentioning

confidence: 99%

Forsythoside A blocks the infection of Avian Infectious Bronchitis Virus through binding S1 subunit

Shen,

Liu,

Yin

et al. 2023

Preprint

View full text Add to dashboard Cite

Avian infectious bronchitis (IB) is an acute highly contagious respiratory infectious disease caused by avian infectious bronchitis virus (IBV), causing serious economic losses to poultry industry. Forsythiaside A (FTA) is a phenylethanol glycoside compound in the fruit of Forsythia suspensa, which is widely used for inflammatory respiratory tract disease, including viral infected inflammation. In this study, the mechanism of FTA against IBV was researched. To determine the inhibitory effect of FTA on IBV infection and the interaction between FTA and IBV particles, immunofluorescence assay, plaque assay, RT-qPCR and enzyme-linked immunosorbent assay (ELISA) were performed and it was discovered that FTA inhibited IBV infection and viral attachment to host receptor under non-cytotoxic concentrations, and FTA may bind to virus particles. Then ELISA and isothermal titration calorimetry (ITC) were employed to further study the interaction between FTA and S protein. The results indicated that FTA bound to S1 subunit rather S2 subunit. Besides, the molecular docking showed that FTA form hydrogen bonds with eight amino acids in the S1 subunit. When these amino acids were mutated. the affinity disappeared between the S1 mutant and FTA. In conclusion, FTA prevents IBV infection by blocking viral attachment and contacts with the S1, but not the S2 subunit of IBV. At last, the bind sites were identified by comparing the affinity of wild S1 and mutant S1.

show abstract

Systematic identification of chemical components in Fufang Shuanghua oral liquid and screening of potential active components against SARS-CoV-2 protease

Cited by 10 publications

References 25 publications

Phytochemicals Characterization of Lianqiao (Forsythia suspensa) extract and evaluation of its antivirus and antioxidant activities

Phytochemicals Characterization of Lianqiao (Forsythia suspensa) extract and evaluation of its antivirus and antioxidant activities

Mass Spectrometric Identification of Metabolites after Magnetic-Pulse Treatment of Infected Pyrus communis L. Microplants

Forsythoside A blocks the infection of Avian Infectious Bronchitis Virus through binding S1 subunit

Contact Info

Product

Resources

About