Systematic identification of seven ribosomal protein genes in bighead carp and their expression in response to microcystin-LR

Cai, Yan; Zhang, Chao; Liang, Hao; Xie, Ping; Chen, Zhidong

doi:10.2131/jts.41.293

Cited by 7 publications

(9 citation statements)

References 35 publications

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“…Prolonged sublethal microcystin exposure decreased the expression of SREBF1 mRNA in mice, which further decreased the expression of CD36 in thyroid dysfunction and metabolic disorders in mice (Clark et al, 2007 ). HNF4A mRNA reduced CD36 expression (Zhao et al, 2015 ), which increases the expression of RPS27A, which then inhibits the expression of CD36 (Cai et al, 2016 ). These predicted observations were validated in our LUAD patient tissues, Figures 7B,C .…”

Section: Discussionmentioning

confidence: 99%

PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin

et al. 2018

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Non-small cell lung cancer (NSCLC) is the major form of lung cancer, with adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC) being its major subtypes. Smoking alone cannot completely explain the lung cancer etiology. We hypothesize that altered lung microbiome and chronic inflammatory insults in lung tissues contribute to carcinogenesis. Here we explore the microbiome composition of LUAD samples, compared to LUSC and normal samples. Extraction of microbiome DNA in formalin-fixed, paraffin-embedded (FFPE) lung tumor and normal adjacent tissues was meticulously performed. The 16S rRNA product from extracted microbiota was subjected to microbiome amplicon sequencing. To assess the contribution of the host genome, CD36 expression levels were analyzed then integrated with altered NSCLC subtype-specific microbe sequence data. Surprisingly phylum Cyanobacteria was consistently observed in LUAD samples. Across the NSCLC subtypes, differential abundance across four phyla (Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes) was identified based on the univariate analysis (p-value < 6.4e-4 to 3.2e-2). In silico metagenomic and pathway analyses show that presence of microcystin correlates with reduced CD36 and increased PARP1 levels. This was confirmed in microcystin challenged NSCLC (A427) cell lines and Cyanobacteria positive LUAD tissues. Controlling the influx of Cyanobacteria-like particles or microcystin and the inhibition of PARP1 can provide a potential targeted therapy and prevention of inflammation-associated lung carcinogenesis.

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Section: Discussionmentioning

confidence: 99%

PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin

et al. 2018

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“…An increased demand in ribosomes may be required in response to stress by translating specific proteins to compensate for potential adverse cellular effects. This was observed in bighead carp ( Aristichthys nobilis ) exposed to microcystins and in Arctic charr ( Salvelinus alpinus ) recovering from heat stress (Quinn et al 2011; Cai et al 2016).…”

Section: Resultsmentioning

confidence: 89%

Food‐Borne Exposure of Juvenile Rainbow Trout (Oncorhynchus mykiss) to Benzotriazole Ultraviolet Stabilizers Alone and in Mixture Induces Specific Transcriptional Changes

Giraudo

Colson

Silva

et al. 2020

Enviro Toxic and Chemistry

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Benzotriazole ultraviolet‐stabilizers (BZT‐UVs) are commonly used as additives to protect from light‐induced degradation in a variety of consumer goods. Despite their widespread presence in aquatic ecosystems, information on the effects of these compounds remains largely unknown. The objectives of the present study were to evaluate the chronic effects of 2 BZT‐UVs alone and in a mixture, 2‐(2H‐benzotriazol‐2‐yl)‐4,6‐bis(1‐methyl‐1‐phenylethyl)phenol (UV‐234) and 2‐(2H‐benzotriazol‐2‐yl)‐4,6‐di‐tert‐pentylphenol (UV‐328), in juvenile rainbow trout (Oncorhynchus mykiss) chronically exposed (for 28 d) through the diet. Chemical analyses of livers from exposed trout suggested liver accumulation and potential metabolism of the 2 compounds. Hepatic RNA‐sequencing analyses revealed specific effects of each compound on gene transcription profiles; UV‐234 affected mainly genes involved in cellular metabolism, whereas UV‐328 induced the transcription of ribosomal proteins and downregulated genes involved in immune responses. Both compounds regulated iron homeostasis genes in an opposite manner. The mixture of both BZT‐UVs did not produce significant evidence of additive or synergistic effects. Environ Toxicol Chem 2020;39:852–862. © 2020 Her Majesty the Queen in Right of Canada. Environmental Toxicology and Chemistry © 2020 SETAC

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“…One of the primary functions of RPL23A is to synthesize ribosomes and to act as a scaffold that enhances the catalytic ability of rRNA to synthesize protein (Cai et al . ). Based on these previous studies, we inferred that RPL23A might be a positive regulation gene for milk protein production.…”

Section: Discussionmentioning

confidence: 97%

Genetic association of DDIT3, RPL23A, SESN2 and NR4A1 genes with milk yield and composition in dairy cattle

Li¹,

Han²,

Liu³

et al. 2019

Animal Genetics

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Previously, we identified by RNA sequencing that DDIT3, RPL23A, SESN2 and NR4A1 genes were significantly differentially expressed between the mammary glands of lactating Holstein cows with extremely high and low milk protein and fat percentages; thus, these four genes are considered as promising candidates potentially affecting milk yield and composition traits in dairy cattle. In the present study, we further verified whether these genes have genetic effects on milk traits in a Chinese Holstein population. By re-sequencing part of the noncoding and the entire coding regions of the DDIT3, RPL23A, SESN2 and NR4A1 genes, a total of 35 SNPs and three insertions/deletions were identified, of which three were found in DDIT3, 12 in RPL23A, 16 in SESN2 and seven in NR4A1. Moreover, two of the insertions/ deletions-g.125714860_125714872del and g.125714806delinsCCCC in SESN2-were novel and have not been reported previously. Subsequent single SNP analyses revealed multiple significant association with all 35 SNPs and three indels regressed against the dairy production traits (P-value = <0.0001-0.0493). In addition, with a linkage disequilibrium analysis, we found one, one, three, and one haplotype blocks in the DDIT3, RPL23A, SESN2 and NR4A1 genes respectively. Haplotype-based association analyses revealed that some haplotypes were also significantly associated with milk production traits (Pvalue = <0.0001-0.0461). We also found that 12 SNPs and two indels (two in DDIT3, two in RPL23A, nine in SESN2 and one in NR4A1) altered the specific transcription factor binding sites in the promoter, thereby regulating promoter activity, suggesting that they might be promising potential functional variants for milk traits. In summary, our findings first determined the genetic associations of DDIT3, RPL23A, SESN2 and NR4A1 with milk yield and composition traits in dairy cattle and also suggested potentially causal variants, which require in-depth validation.

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Systematic identification of seven ribosomal protein genes in bighead carp and their expression in response to microcystin-LR

Cited by 7 publications

References 35 publications

PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin

PARP1 Is Up-Regulated in Non-small Cell Lung Cancer Tissues in the Presence of the Cyanobacterial Toxin Microcystin

Food‐Borne Exposure of Juvenile Rainbow Trout (Oncorhynchus mykiss) to Benzotriazole Ultraviolet Stabilizers Alone and in Mixture Induces Specific Transcriptional Changes

Genetic association of DDIT3, RPL23A, SESN2 and NR4A1 genes with milk yield and composition in dairy cattle

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