Systematic Investigation of Plant-Parasitic Nematodes Associated with Main Subtropical Crops in Guangxi Province, China

Mo, Yixue; Mo, Ai-Su; Qiu, Zhuo-Qiu; Li, Bingxue; Wu, Haiyan

doi:10.3390/life11111177

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…The zigzag sampling method was used to obtain 50 soil cores with a diameter of 2.5 cm and a depth of 20 cm. The H. zeae cysts were extracted from soil by the modi ed sucrose-otation and centrifugation technique (Mo et al 2021). Cysts were surface disinfection with 0.5% NaOCl for 3 min and rinsed with sterile water.…”

Section: Sample Collection and Strain Isolationmentioning

confidence: 99%

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Novel ectophytic nematode antagonistic fungi and its potential application for nematode biocontrol

Kan

Jia

et al. 2022

Preprint

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Dynamic of plant-parasitic nematodes population in soil is closely related to soil microorganisms. Fungi from Heterodera zeae cysts were isolated to explore the natural decline phenomenon of H. zeae population. A polyphasic method was used to identify the nematode-destroying fungal, including phylogenetic analysis of partial ITS, BenA, CaM, and RPB2 gene sequences, as well as macro- and micro-morphological analyses. The nematicidal activity of strain GX1 against H. zeae was assessed in vitro and greenhouse. Our findings revealed that strain GX1 is a novel species of Talaromyces, which named Talaromyces cystophila. It had strong parasitic and lethal effect on the H. zeae cysts with 91.11% parasitism on cysts at 3 d after treatment, and second-stage juveniles (J2s) and eggs inside the cysts appeared dense vacuoles and loss of vitality. The spore suspension and fermentation filtrate of T. cystophila inhibited significantly the hatching of H. zeae eggs and showed potential nematicidal activities on J2s. The hatching inhibition rates of eggs exposed to 1 × 108 cfu/ml spore suspensions or 20% 1-week fermentation filtrate (1-WF) for 15 days were 98.56% and 100%, respectively. The mortality of J2s exposed to 1 × 108 cfu/ml spore suspension reached 100% at 24 h, exposure to 50% 2-WF was 98.65% and 100% at 24 h and 48 h, respectively. Greenhouse experiments revealed that the spore suspension and fermentation broth considerably decreased H. zeae reproduction by 56.17%-78.76%. T. Cystophila is a potential biocontrol strain with parasitic and nematicidal activity, which deserves attention and application.

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Section: Sample Collection and Strain Isolationmentioning

confidence: 99%

“…H. zeae was cultured on maize (Zhengda 619), and cysts were collected at 40 days after inoculation Cysts were processed as described by Mo et al (2021). Cysts were hand-picked with a dissecting needle under a stereomicroscope (SZX2, ILLT, Olympus).…”

Section: Nematode Preparationmentioning

confidence: 99%

Novel ectophytic nematode antagonistic fungi and its potential application for nematode biocontrol

Kan

Jia

et al. 2022

Preprint

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show abstract

“…This study was the first report of D. destructor causing stem rot of maize in Heilongjiang province, China. Mo et al [ 2 ] performed the systematic investigation of PPNs associated with main subtropical crops in Guangxi province, China. Through analysis of 425 soil and root samples from crops, including sugarcane, rice, maize, and soybean, they identified a total of 48 order/family/genera of PPNs.…”

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confidence: 99%