Systematic investigation of the toxic mechanism of difenoconazole on protein by spectroscopic and molecular modeling

Liu, Ying; Ma, Xueru; Lu, Guanghua

doi:10.1016/j.pestbp.2012.12.010

Cited by 17 publications

(6 citation statements)

References 23 publications

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“…Through this experiment, it can be concluded that the fungal isolates were able to break down and analyse the pesticide. These results are in a good accordance with those of some studies confirming that the fungicide Difenoconazole inhibits fungal spore growth, as the pesticide Difenoconazole effectively inhibits fungal spore growth and prevents fungal proliferation [23] and [22]. A B…”

Section: Effect Of Difenoconazole Concentrations On the Fungal Sporul...supporting

confidence: 91%

“…It is a systemic fungicide used to control plant diseases and has eradicating and preventive effects. It acts through penetration and works to prevent reproduction and inhibit growth [22] and [23]. This fungicide is characterized by its high chemical stability, chemical stability to high light, low biodegradability and significant ability to move easily in the environment [24].…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of Difenoconazole Using Fungal-Bacterial Consortia

Wared Musa,

Abass,

Al-Farttoosy

2024

IOP Conf. Ser.: Earth Environ. Sci.

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Pesticides are used all over the world in agricultural operations to reduce the damage of pests and to improve the productivity of agricultural crops. The excessive use of these chemical compounds has led to the deterioration of soil and increase the level of pollution of water and air resources. Moreover, the continuous use and non-target toxicity of pesticides has become a major concern to the agricultural ecosystem that directly hinders the productivity of agricultural products. Therefore, this study aimed to isolate and identify fungal and bacterial species with the ability to biodegrade fungicide via conducting preliminary tests to find out which of them have the ability to grow in a treated medium with a fungicide Difenoconazole, which belongs to the group of the pesticides Triazoles. The fungi and bacteria that succeeded in growing were as follow Aspergillus flavus, Aspergillus ochraceus, Bacillus cereus S1 and Bacillus cereus S2 were identified by extracting their genomic DNA and applying the primers ITS and 16s rRNA for both examined fungi and bacteria, respectively. The fungi were laboratory adapted to multiple concentrations reaching the highest concentration of 550 mgL-1, while bacteria reached 1200 mgL-1 laboratory to break down the pesticide. The results of the combinations tested as the B. cereus S1 and B. cereus S2 were the fastest growing in the liquid medium PDB at the concentration of 325 mgL-1. While the combination A. flavus and B. cereus S1 had the highest biodegrading ability at the same concentration, which led to the disappearance of the pesticide Difenoconazole turbidity in the liquid medium as a result of its consumption by fungal and bacterial isolates. As for the treatment of the pesticide in the solid PDA medium to know its effect on the growth of fungi and the formation of spores, the results revealed that fungal isolates increase their vegetative growth when the concentration increases, the production of fungal spores decreases, and indicates the ability of the fungi to use the pesticide as an energy source.

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Section: Effect Of Difenoconazole Concentrations On the Fungal Sporul...supporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Biodegradation of Difenoconazole Using Fungal-Bacterial Consortia

Wared Musa,

Abass,

Al-Farttoosy

2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…Pydiflumetofen is a succinate dehydrogenase inhibitor (SDHI) that disrupts energy production [60]. Difenoconazole is a sterol demethylation inhibitor that inhibits cell membrane ergosterol biosynthesis [28]. It has been reported that Meitian can effectively inhibit rose powdery mildew and cucumber powdery mildew in the field [61,62].…”

Section: Discussionmentioning

confidence: 99%

“…SDHI fungicides bind to the succinate dehydrogenase (SDH) complex and block the transport of electrons in the respiratory chain which are necessary to produce ATP, curtailing energy production and arresting fungal growth [27]. Difenoconazole is a sterol demethylation inhibitor that inhibits cell membrane ergosterol biosynthesis [28].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Control of Dendrobium officinale Bud Blight Disease

et al. 2023

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Dendrobium officinale is an important traditional Chinese medicine (TCM). A disease causing bud blight in D. officinale appeared in 2021 in Yueqing city, Zhejiang Province, China. In this paper, 127 isolates were obtained from 61 plants. The isolates were grouped into 13 groups based on collected areas and morphological observations. Four loci (ITS, LSU, tub2 and rpb2) of 13 representative isolates were sequenced and the isolates were identified by constructing phylogenetic trees with the multi-locus sequence analysis (MLSA) method. We found the disease to be associated with three strains: Ectophoma multirostrata, Alternaria arborescens and Stagonosporopsis pogostemonis, with isolates frequencies of 71.6%, 21.3% and 7.1%, respectively. All three strains are pathogenic to D. officinale. A. arborescens and S. pogostemonis isolated from D. officinale were reported for the first time. Iprodione (50%), 33.5% oxine-copper and Meitian (containing 75 g/L pydiflumetofen and 125 g/L difenoconazole) were chosen to control the dominant pathogen E. multirostrata, with an EC50 value of 2.10, 1.78 and 0.09 mg/L, respectively. All three fungicides exhibited an effective inhibition of activities to the growth of the dominant pathogen E. multirostrata on potato dextrose agar (PDA) plates, with Meitian showing the strongest inhibitory effect. We further found that Meitian can effectively control D. officinale bud blight disease in pot trial.

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“…Difenoconazole being a cis, trans-3-chloro-4-[4-methyl-2-(1H-1,2,4-triazol-1-ylmethyl)-1,3dioxolan-2-yl]phenyl-4 -chlorophenylether) phenyl-4-chlorophenylether, known commercially as Score ® , belongs to the triazole group of pesticides, being a systemic inhibitor of sterol demethylation (DMI), which has the ability to interfere with mycelial growth and inhibit the germination of spores of pathogens that ultimately result in the inhibition of fungal growth (Wang et al, 2019;Li et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the association of 8-hydroxyquinoline with fungicides against phytopathogenic fungi in rice seeds

Kaminski¹,

Carvalho²,

Lopes³

et al. 2022

Rev Agr Acad

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Evaluation of the association of 8-hydroxyquinoline with commercial fungicides against phytopathogenic fungi where it was possible to verify the synergism between the compounds and the antifungal, which led to in vivo tests using rice seeds. The association of 0.5 g/L of mancozeb and 0.031 g/L of 8-hydroxyquinoline, concentration 4 and 8 times lower than the one usually used, respectively, was promising. The test of leukocyte cytotoxicity and dermal toxicity was carried out, showing, in view of all the tests performed, a potential selective agent against variations of Fusarium graminearum and Fusarium meridionale in rice seeds.

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Systematic investigation of the toxic mechanism of difenoconazole on protein by spectroscopic and molecular modeling

Cited by 17 publications

References 23 publications

Biodegradation of Difenoconazole Using Fungal-Bacterial Consortia

Biodegradation of Difenoconazole Using Fungal-Bacterial Consortia

Characterization and Control of Dendrobium officinale Bud Blight Disease

Evaluation of the association of 8-hydroxyquinoline with fungicides against phytopathogenic fungi in rice seeds

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