Systematic placement of the Turkish endemic genus Ekimia (Apiaceae)based on morphological and molecular data

Lyskov, Dmitry; Degtjareva, Galina V.; Samigullin, Tahir H.; Пименов, М. Г.

doi:10.3906/bot-1405-111

Cited by 12 publications

(11 citation statements)

References 18 publications

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“…The paraphyly of the genus Daucus (circumscribed containing Rouya polygama ) was extensively documented using plastid or ribosomal DNA characters [35, 37]. Recent studies [37, 112], using nrDNA ITS sequences and plastid sequences also showed that Rouya polygama groups within the Daucus clade.…”

Section: Discussionmentioning

confidence: 99%

Genotyping-by-sequencing provides the discriminating power to investigate the subspecies of Daucus carota (Apiaceae)

et al. 2016

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BackgroundThe majority of the subspecies of Daucus carota have not yet been discriminated clearly by various molecular or morphological methods and hence their phylogeny and classification remains unresolved. Recent studies using 94 nuclear orthologs and morphological characters, and studies employing other molecular approaches were unable to distinguish clearly many of the subspecies. Fertile intercrosses among traditionally recognized subspecies are well documented. We here explore the utility of single nucleotide polymorphisms (SNPs) generated by genotyping-by-sequencing (GBS) to serve as an effective molecular method to discriminate the subspecies of the D. carota complex.ResultsWe used GBS to obtain SNPs covering all nine Daucus carota chromosomes from 162 accessions of Daucus and two related genera. To study Daucus phylogeny, we scored a total of 10,814 or 38,920 SNPs with a maximum of 10 or 30% missing data, respectively. To investigate the subspecies of D. carota, we employed two data sets including 150 accessions: (i) rate of missing data 10% with a total of 18,565 SNPs, and (ii) rate of missing data 30%, totaling 43,713 SNPs. Consistent with prior results, the topology of both data sets separated species with 2n = 18 chromosome from all other species. Our results place all cultivated carrots (D. carota subsp. sativus) in a single clade. The wild members of D. carota from central Asia were on a clade with eastern members of subsp. sativus. The other subspecies of D. carota were in four clades associated with geographic groups: (1) the Balkan Peninsula and the Middle East, (2) North America and Europe, (3) North Africa exclusive of Morocco, and (4) the Iberian Peninsula and Morocco. Daucus carota subsp. maximus was discriminated, but neither it, nor subsp. gummifer (defined in a broad sense) are monophyletic.ConclusionsOur study suggests that (1) the morphotypes identified as D. carota subspecies gummifer (as currently broadly circumscribed), all confined to areas near the Atlantic Ocean and the western Mediterranean Sea, have separate origins from sympatric members of other subspecies of D. carota, (2) D. carota subsp. maximus, on two clades with some accessions of subsp. carota, can be distinguished from each other but only with poor morphological support, (3) D. carota subsp. capillifolius, well distinguished morphologically, is an apospecies relative to North African populations of D. carota subsp. carota, (4) the eastern cultivated carrots have origins closer to wild carrots from central Asia than to western cultivated carrots, and (5) large SNP data sets are suitable for species-level phylogenetic studies in Daucus.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-016-0806-x) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Genotyping-by-sequencing provides the discriminating power to investigate the subspecies of Daucus carota (Apiaceae)

et al. 2016

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“…However, a detailed comparison of fruit anatomy of Ekimia and its presumed relatives (two species of Prangos, P. ferulacea Lindl. and P. lophoptera Boiss., and an Anatolian representative of Laserpitium, L. petrophilum) demonstrated that Ekimia shares several characters with Laserpitium petrophilum (Lyskov & al., 2015). Its position in Daucinae is therefore firmly established both by molecular and morphological data.…”

Section: Version Of Recordmentioning

confidence: 99%

Phylogeny of Apiaceae subtribe Daucinae and the taxonomic delineation of its genera

Banasiak

Wojewódzka

Baczyński

et al. 2016

TAXON

103

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Scandiceae subtribe Daucinae encompasses umbellifers that have fruits with prominent secondary ridges projecting into wings (former tribe Laserpitieae) or spines (former tribe Caucalideae pro parte). It comprises several economically or medicinally important genera including Cuminum, Daucus, Laser, Laserpitium and Thapsia among others. Recent molecular studies, based mostly on nrDNA ITS sequences, revealed that neither Daucus nor Laserpitium are monophyletic. To address issues of relationships and apply respective nomenclatural changes, we obtained additional ITS sequences as well as independent data from three plastid markers—rps16 intron, rpoC1 intron and rpoB‐trnC intergenic spacer—for a comprehensive sample of the subtribe. We examined data for 260 accessions representing all genera of Daucinae and 81 of its ca. 93 species. Phylogenetic trees were estimated using maximum likelihood and Bayesian inference methods. The results indicate that former Laserpitieae constitute a paraphyletic grade at the base of the spiny‐fruited members of Daucinae while traditionally delimited Daucus and Laserpitium are polyphyletic. To maintain a monophyletic Daucus, we suggest including the following genera and species into its synonymy: Agrocharis, Melanoselinum, Monizia, Pachyctenium, Pseudorlaya, Rouya, Tornabenea, Athamanta dellacellae and Cryptotaenia elegans. The species of Laserpitium occur in seven clades and only six species of the Laserpitium s.str. clade retain the generic name. Several species are transferred to Ekimia, Laser and Thapsia; additionally, a monospecific genus Siler is restored and a new genus, Silphiodaucus, is established. The inclusion of Ammodaucus into Thapsia suggested in an earlier study is not supported. The position of Laserpitium pseudomeum requires further study.

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“…Fruit characters are considered crucial in taxonomy throughout the whole Apiaceae as can be seen in any identification key (Engler 1927). The possibility of better characterizing fruits by using both anatomical characters and micromorphological features using SEM has stimulated numerous studies across the family in genera such as Bupleurum L. (Özcan 2004), Ferulago Koch (Akalın & Kızılarslan 2013), Ekimia H.Duman & M.F.Watson (Lyskov & al. 2015), Grammasciadium DC.…”

Section: Introductionmentioning

confidence: 99%

Fruit morphology of the genus <em>Pimpinella (Apiaceae)</em> in Turkey

Yeşıl

Akalin

Akpulat

et al. 2018

Anal. Jard. Bot. Madr.

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To explore if fruit morphology could aid in taxonomy of the genus Pimpinella L., we have undertaken a study of fruits from 26 Turkish taxa of Pimpinella using light and scanning electron microscopy—SEM—. A great deal of inter and intraspecific variation for both fruit shape and surface was observed. Fruit shapes of Turkish taxa of Pimpinella range from oblong-cylindrical to subglobose and indumentum when present can be strigose, hispid and may include hamate trichomes. Variation in fruit surface is also considerable and allows recognizing nine different ornamentation patterns. However, variation in shape, surface ornamentation and indumentum is not tightly associated since species with similar fruit shapes do not necessarily have similar surface ornamentation. To jointly analyse fruit morphology together with the most commonly used morphological characters of the whole plant and to compare morphological evidence with available phylogenetic hypotheses, a cluster analysis was also performed: the Turkish species of Pimpinella were clustered into two distinct groups, the second one subdivided in another two subgroups.

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Systematic placement of the Turkish endemic genus Ekimia (Apiaceae)based on morphological and molecular data

Cited by 12 publications

References 18 publications

Genotyping-by-sequencing provides the discriminating power to investigate the subspecies of Daucus carota (Apiaceae)

Genotyping-by-sequencing provides the discriminating power to investigate the subspecies of Daucus carota (Apiaceae)

Phylogeny of Apiaceae subtribe Daucinae and the taxonomic delineation of its genera

Fruit morphology of the genus <em>Pimpinella (Apiaceae)</em> in Turkey

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