Systematic Review on the Methods of the Mitotic Count used in
                    Prognostic Studies on canine Tumors

Bertram, CA; Fuchs‐Baumgartinger, Andrea; Donovan, TA

doi:10.1055/s-0042-1749993

Cited by 1 publication

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“…In diagnostic veterinary pathology, the number of dividing cells (mitotic figures, MFs) per tumor area, referred to as the mitotic count (MC), is commonly determined in routine histological sections. For some canine and feline neoplastic entities, including canine cutaneous mast cell tumors (ccMCT), the MC has been shown to be prognostically meaningful [1][2][3][4][5][6][7][8][9]. For ccMCT, the MC is also part of the well-established, two-tier histologic grading system published in 2011 [10] and the older, three-tier histologic grading system published in 1984 [11], and thus is routinely determined for each diagnostic case.…”

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confidence: 99%

Atypical Mitotic Figures Are Prognostically Meaningful for Canine Cutaneous Mast Cell Tumors

Bertram,

Bartel,

Donovan

et al. 2023

Veterinary Sciences

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Cell division through mitosis (microscopically visible as mitotic figures, MFs) is a highly regulated process. However, neoplastic cells may exhibit errors in chromosome segregation (microscopically visible as atypical mitotic figures, AMFs) resulting in aberrant chromosome structures. AMFs have been shown to be of prognostic relevance for some neoplasms in humans but not in animals. In this study, the prognostic relevance of AMFs was evaluated for canine cutaneous mast cell tumors (ccMCT). Histological examination was conducted by one pathologist in whole slide images of 96 cases of ccMCT with a known survival time. Tumor-related death occurred in 11/18 high-grade and 2/78 low-grade cases (2011 two-tier system). The area under the curve (AUC) was 0.859 for the AMF count and 0.880 for the AMF to MF ratio with regard to tumor-related mortality. In comparison, the AUC for the mitotic count was 0.885. Based on our data, a prognostically meaningful threshold of ≥3 per 2.37 mm2 for the AMF count (sensitivity: 76.9%, specificity: 98.8%) and >7.5% for the AMF:MF ratio (sensitivity: 76.9%, specificity: 100%) is suggested. While the mitotic count of ≥ 6 resulted in six false positive cases, these could be eliminated when combined with the AMF to MF ratio. In conclusion, the results of this study suggests that AMF enumeration is a prognostically valuable test, particularly due to its high specificity with regard to tumor-related mortality. Additional validation and reproducibility studies are needed to further evaluate AMFs as a prognostic criterion for ccMCT and other tumor types.

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