Systematic revision of <i>Hydnum</i> species in Japan

Sugawara, Ryo; Maekawa, Nitaro; Sotome, Kozue; Nakagiri, Akira; Endo, Naoki

doi:10.1080/00275514.2021.2024407

Cited by 8 publications

(30 citation statements)

References 47 publications

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“…We examined 33 basidiomata specimens of H. boreorepandum and H. repando-orientale s. lat. (Table 1): 23 were collected in our studies in 2016-2020 (Sugawara et al 2019(Sugawara et al , 2022a); 4 were collected in this study; 2, including holotype of H. repando-orientale (TUMH 60745), were loaned from the Tottori University Mycological Herbarium (TUMH), Fungus/Mushroom Resource and Research Center, Faculty of Agriculture, Tottori University; 4 were loaned from the TNS herbarium of the National Museum of Nature and Sciences, Tokyo. These specimens were collected at 25 sites in Honshu, mainland Japan, at 10 to 2500 m a.s.l.…”

Section: Basidiomata Specimensmentioning

confidence: 99%

“…The morphological characterization was conducted in accordance with the method in our previous report (Sugawara et al 2022a). Thirty basidiospores were measured from each of 21 specimens, and previous measurement were reused for 19 specimens.…”

Section: Morphological Analysismentioning

confidence: 99%

“…DNA extraction, PCR ampli cation, and sequencing analysis were performed as described by Sugawara et al (2022a). ITS and LSU amplicons were obtained using universal primers for basidiomycetes: ITS1F/ LBW (Gardes and Bruns 1993;Tedersoo et al 2008) and CTB6/ LR5F (Garbelotto et al 1997;Tedersoo et al 2008).…”

Section: Dna Extraction Polymerase Chain Reaction (Pcr) Ampli Cation ...mentioning

confidence: 99%

“…ITS and LSU amplicons were obtained using universal primers for basidiomycetes: ITS1F/ LBW (Gardes and Bruns 1993;Tedersoo et al 2008) and CTB6/ LR5F (Garbelotto et al 1997;Tedersoo et al 2008). TEF1 and RPB1 amplicons were obtained using primers for Hydnum species [RPB1: Hrpb1F or Hrpb1-4F/ Hrpb1-2R or Hrpb1R (Feng et al 2016)] [TEF1: HydTEF1-F/ HydTEF1-R (Sugawara et al 2022a)]. For PCR ampli cation of RPB2, we rst ampli ed and sequenced several Hydnum and ectomycorrhizal Sistotrema using universal primers [fRPB2-5F or bRPB2-6F/ RPB2-b7R2 or RPB2-b7.1R (Kretzer and Bruns 1999;Liu et al 1999)], then designed new primers based on the obtained sequences: HRPB2-5.5F (forward: 5'-GNAAYTGGGGBGACCAGAAG-3'), HRPB2-5.6F (forward: 5'-AAGGCWGGYGTRTCCCAGGT-3'), HRPB2-6.8R (reverse: 5'-GGRTGRATCTCRCAATGTGTCCA-3'), HRPB2-6.9R (reverse: 5'-GRTGRATCTCRCAATGTGTCCA-3').…”

Section: Dna Extraction Polymerase Chain Reaction (Pcr) Ampli Cation ...mentioning

confidence: 99%

“…The Hydnum species classi cation emphasizes on the phylogenetic relationships inferred from ITS sequences (Niskanen et al 2018; Swenie et al 2018;Cao et al 2021b;Sugawara et al 2022a); however, a phylogeny of the non-coding ITS region alone sometimes leads to unreliable data because it depends on the evolutionary history of a single DNA sequence, rather than a species. For this reason, the use of multiple molecular markers is strongly recommended in a taxonomic framework (Lücking et al 2020;Aime et al 2021;Cao et al 2021a).…”

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confidence: 99%

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Ecological speciation of Japanese hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

et al. 2022

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Hydnum repando-orientale is an East Asian species closely related to H. boreorepandum and H. repandum; all three species produce edible mushrooms. We identi ed two ecological groups of H. repando-orientale in Japan: a temperate group occurring in Fagaceae-dominated forest at < 1200 m a.s.l. (ROF), and a subalpine group occurring in coniferous forest in highland at > 1900 m a.s.l. (ROC). We re-examined the taxonomy of the two ecological groups of H. repando-orientale using integrative approaches. Phylogenies of the two ecological groups and other related species were inferred from the internal transcribed spacer (ITS) and gene portions encoding the large subunit of nc rRNA (LSU), translation elongation factor-1 alpha (TEF1), RNA polymerase II largest subunit (RPB1), and RNA polymerase II second-largest subunit (RPB2).

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Section: Basidiomata Specimensmentioning

confidence: 99%

Section: Morphological Analysismentioning

confidence: 99%

Section: Dna Extraction Polymerase Chain Reaction (Pcr) Ampli Cation ...mentioning

confidence: 99%

Section: Dna Extraction Polymerase Chain Reaction (Pcr) Ampli Cation ...mentioning

confidence: 99%

mentioning

confidence: 99%

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Ecological speciation of Japanese hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

et al. 2022

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Unveiling the Diversity of Hydnum in the Republic of Korea with One New Species, Hydnum paucispinum

Kim,

Lee,

Kim

et al. 2023

Mycobiology

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Hydnum is a genus of ectomycorrhizal fungi belonging to the Hydnaceae family. It is widely distributed across different regions of the world, including North America, Europe, and Asia; however, some of them showed disjunct distributions. In recent years, with the integration of molecular techniques, the taxonomy and classification of Hydnum have undergone several revisions and advancements. However, these changes have not yet been applied in the Republic of Korea. In this study, we conducted an integrated analysis combining the morphological and molecular analyses of 30 specimens collected over a period of approximately 10 years in the Republic of Korea. For molecular analysis, the sequence data of the internal transcribed spacer (ITS) region, the large subunit of nuclear ribosomal RNA gene (nrLSU), and a portion of translation elongation factor 1-α ( TEF1 ) were employed as molecular markers. Through this study, we identified eight species that had previously not been reported to occur in the Republic of Korea, including one new species, Hydnum paucispinum . A taxonomic key and detailed descriptions of the eight Hydnum species are provided in this study.

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Ecological speciation of Japanese Hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

Sugawara

Aoki²,

Yamada³

et al. 2022

Preprint

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Hydnum repando-orientale is an East Asian species closely related to H. boreorepandum and H. repandum; all three species produce edible mushrooms. We identified two ecological groups of H. repando-orientale in Japan: a temperate group occurring in Fagaceae-dominated forest at < 1200 m a.s.l. (ROF), and a subalpine group occurring in coniferous forest in highland at > 1900 m a.s.l. (ROC). We re-examined the taxonomy of the two ecological groups of H. repando-orientale using integrative approaches. Phylogenies of the two ecological groups and other related species were inferred from the internal transcribed spacer (ITS) and gene portions encoding the large subunit of nc rRNA (LSU), translation elongation factor-1 alpha (TEF1), RNA polymerase II largest subunit (RPB1), and RNA polymerase II second-largest subunit (RPB2). The concatenated phylogenetic tree separated the two ecological groups into well-supported sister clades. Also, species delimitations based on the topological congruence (GCPSR) and multispecies coalescent model (GMYC and BP&P) supported to separate the two ecological groups. Morphological analysis showed that ROC specimens had significantly larger basidiospores, compared with ROF specimens. Mon-mon mating tests using six ROF, three ROC, and three H. boreorepandum strains each showed independent incompatible groups, whereas one ROC strain showed compatibility with both ROC and ROF populations. Based on these results, we defined the ROC group as a new species, H. subalpinum. Because H. repando-orientale and H. subalpinum have smaller genetic divergence in nc rDNA and maintain slight sexual compatibility, they may have recently speciated in East Asia.

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Systematic revision of Hydnum species in Japan

Cited by 8 publications

References 47 publications

Ecological speciation of Japanese hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

Ecological speciation of Japanese hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

Unveiling the Diversity of Hydnum in the Republic of Korea with One New Species, Hydnum paucispinum

Ecological speciation of Japanese Hedgehog mushroom: Hydnum subalpinum sp. nov. is distinguished from its sister species H. repando-orientale by means of integrative taxonomy

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