Systematic screening for genes specifically expressed in the anterior neuroectoderm during early Xenopus development

Takahashi, Noriyuki; Tochimoto, Naoko; Ohmori, Shingo; Mamada, Hiroshi; Itoh, Mari; Inamori, Masako; Shinga, Jun; Osada, Shin‐Ichi; Taira, Masanori

doi:10.1387/ijdb.052083nt

Cited by 12 publications

(21 citation statements)

References 43 publications

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“…Our studies show that members of all semaphorin classes analyzed, including the previously observed Sema6D4 (Takahashi et al, 2005), are expressed at the right time and place for a function in mesodermal morphogenesis. Since semaphorins can act as guidance cues, their expression in the mesoderm could affect mesodermal cell movements by providing positional information for cells undergoing convergent extension.…”

Section: Discussionsupporting

confidence: 65%

“…ba, branchial arches; cg, cement gland; e, eye; h, heart; m, somitogenic mesoderm; nt, neural tube; no, notochord; p, pronephros; pcv, posterior cardial vein; pnc, premigratory neural crest cells. Sema6D (Takahashi et al, 2005). At tadpole stages, only Sema3F and Sema4D continue to be expressed in the notochord (Fig.…”

Section: Fig 3 Expression Of Class-4 Semaphorins Inmentioning

confidence: 92%

“…Holt (Campbell et al, 2001) and the Sema6D4 clone by M. Taira (Takahashi et al, 2005). Antisense probes generated from full-length as well as shorter EST constructs of the same semaphorin, produced similar in situ hybridization patterns.…”

Section: Clones For In Situ Hybridizationmentioning

confidence: 98%

“…However, expression in early embryonic morphogenesis has not been systematically addressed. Moreover, to our knowledge the expression pattern of only one Xenopus semaphorin, Sema6D4, has been previously published (Takahashi et al, 2005). Here, we analyzed the expression of 13 members of the semaphorin family during early Xenopus laevis development, focusing on tissues undergoing morphogenetic cell movements, like the migrating neural crest and the gastrulating mesoderm, and thereby providing a useful expression map for future functional analyses.…”

Section: Introductionmentioning

confidence: 99%

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Semaphorin and neuropilin expression during early morphogenesis of Xenopus laevis

Koestner

Shnitsar

Linnemannstöns

et al. 2008

Developmental Dynamics

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Semaphorins are major regulators of morphogenesis and are involved in a variety of processes ranging from the guidance of cell migration to the development of cancer. Since semaphorins were first characterized as repulsive neuronal guidance cues, their expression has been best documented in the nervous system. However, broader studies are lacking. Here, we describe the expression of 13 members of the semaphorin family and two neuropilin receptors during early Xenopus laevis development. No particular expression pattern defines any of the semaphorin classes, but many are dynamically expressed in distinct areas undergoing morphogenetic cell movements like the developing mesoderm and the migrating neural crest. Furthermore, the complementary expression patterns of Sema3A/Nrp1 and Sema3F/Nrp2 are maintained across hundreds of millions of years, possibly indicating a conserved role in the guidance of migrating neural crest cells.

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Section: Discussionsupporting

confidence: 65%

Section: Fig 3 Expression Of Class-4 Semaphorins Inmentioning

confidence: 92%

Section: Clones For In Situ Hybridizationmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Semaphorin and neuropilin expression during early morphogenesis of Xenopus laevis

Koestner

Shnitsar

Linnemannstöns

et al. 2008

Developmental Dynamics

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“…Both of these genes were strongly expressed in the cement gland where densin and scrib were not detected, supporting the possibility of functional interactions between the two molecules. In X. laevis, lano (SCRP1) was isolated in a screen for anterior neuroectoderm expressed genes and was shown to be weakly ubiquitously expressed in the neural plate at the neurula stage [27].…”

Section: Discussionmentioning

confidence: 99%

Dynamic expression of the LAP family of genes during early development of Xenopus tropicalis

Yang

Kong

et al. 2011

Sci. China Life Sci.

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The leucine-rich repeats and PDZ (LAP) family of genes are crucial for the maintenance of cell polarity as well as for epithelial homeostasis and tumor suppression in both vertebrates and invertebrates. Four members of this gene family are known: densin, erbin, scribble and lano. Here, we identified the four members of the LAP gene family in Xenopus tropicalis and studied their expression patterns during embryonic development. The Xenopus LAP proteins show a conserved domain structure that is similar to their homologs in other vertebrates. In Xenopus embryos, these genes were detected in animal cap cells at the early gastrula stage. At later stages of development, they were widely expressed in epithelial tissues that are highly polar in nature, including the neural epithelia, optic and otic vesicles, and in the pronephros. These data suggest that the roles of the Xenopus LAP genes in the control of cell polarity and morphogenesis are conserved during early development. Erbin and lano show similar expression patterns in the developing head, suggesting potential functional interactions between the two molecules in vivo. . The PDZ domain, consisting of six -strands and two -helices, is believed to mediate protein-protein interactions. Densin (also known as leucine rich repeat containing 7, Lrrc7) was originally identified from rat forebrain and was the first LAP protein to be characterized [6]. In mammals, Densin interacts with tissue specific proteins to participate in the formation of synaptic junctions in the brain and in the slit diaphragm in the glomus of kidney [7][8][9]. Erbin interacts with various proteins and has been shown to be important for neural development and epithelial homeostasis [10,11]. Scrib is well known as a polarity protein and a tumor suppressor in both vertebrates and invertebrates

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Microtubule interfering agents and KSP inhibitors induce the phosphorylation of the nuclear protein p54nrb, an event linked to G2/M arrest

Casado

Prado

Zuazua-Villar

et al. 2009

Journal of Proteomics

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Microtubule interfering agents (MIAs) are anti-tumor drugs that inhibit microtubulearrest and cell death [3,5]. Nowadays, the most used MIAs for 49 cancer treatment are vinca alkaloids (VAs) and taxanes [6]. 50VAs are drugs derived from the periwinkle Catharanthus Gel spots were subjected to in-gel digestion (http://msfacility. 161ucsf.edu/ingel.html) with trypsin (porcine, side-chain removed using a Y-10 microcone (Millipore), proteins were (Fig. 1a). induced forms are phosphorylated (Fig. 2). Spot x also disappeared 246 after λ-PPase treatment and therefore, this form is also con-247 sidered as phosphorylated (Fig. 2) (Fig. 3a). 260These phosphorylations were also confirmed by Western blot 261 analysis (see Fig. 1 of supplementary material). As expected, 262the flow cytometry analysis of these cells showed that all the 263 compounds used induced G2/M arrest and cell death (Fig. 3b). 264Furthermore, a Western blot analysis using antibodies against 265 the molecular marker phospho-histone H3 indicated that the 266 G2/M arrest induced by these agents is at M stage (Fig. 3c). (Fig. 4b) and M phase arrest (Fig. 4c). More interestingly, (Fig. 4a) (Fig. 5a). This downregulation is coupled to a reduction in the 296 number of M phase cells (Fig. 5c) Aphidicolin was added 24 h prior to MIA treatment. Then, cell 312 extracts were subjected to 2D-PAGE followed by Western blot. (Fig. 7b). This drug also induces the spots x, y and z which 337 correspond to processed forms of the protein (Fig. 7b). 338Roscovitine treatment induced p54 nrb processing but did not 339 affect its phosphorylation (Fig. 7b). Interestingly, the addition of (Fig. 8a). In addition, a Western blot analysis using antibodies 354 against phospho-histone H3 indicated that MIAs also cause M 355 arrest in this cell line (Fig. 8b). The KSP inhibitor STLC also 356 induced the phosphorylation of this nuclear factor (see Fig. 2 357 of supplementary material). 4. Discussion

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Systematic screening for genes specifically expressed in the anterior neuroectoderm during early Xenopus development

Cited by 12 publications

References 43 publications

Semaphorin and neuropilin expression during early morphogenesis of Xenopus laevis

Semaphorin and neuropilin expression during early morphogenesis of Xenopus laevis

Dynamic expression of the LAP family of genes during early development of Xenopus tropicalis

Microtubule interfering agents and KSP inhibitors induce the phosphorylation of the nuclear protein p54nrb, an event linked to G2/M arrest

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