Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

Wei-xin, LI; Wang, Huan; Dong, Anjie

doi:10.3390/pr7120924

Cited by 11 publications

(2 citation statements)

References 23 publications

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“…Extraction steps were slightly modified from the literature [60]. A total of 100 g of heartwood powder was dissolved in 1000 mL of ethanol (95%, v/v) and extracted for 2 h. Extracted twice, the filtrate was concentrated and dried to obtain the crude extract.…”

Section: Non-volatile Compoundsmentioning

confidence: 99%

A Study of the Chemical Composition and Biological Activity of Michelia macclurei Dandy Heartwood: New Sources of Natural Antioxidants, Enzyme Inhibitors and Bacterial Inhibitors

Chen

Wei

2023

IJMS

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The wood of Michelia macclurei Dandy (MD) is an excellent material that is widely used in the furniture, handicraft, and construction industries. However, less research has been conducted on the chemical composition and biological activity of heartwood, which is the main valuable part of the wood. This study aimed to investigate the chemical composition and biological activities of the heartwood of Michelia macclurei Dandy (MDHW) and to confirm the active ingredients. Triple quadrupole gas chromatography–mass spectrometry (GC-MS) was used to characterize the volatile components of MDHW, while ultra-performance liquid chromatography–mass spectrometry was used to analyze the non-volatile components (UPLC-MS). The total reducing power, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging assays, acetylcholinesterase and α-glucosidase inhibition assays, and an antimicrobial test of 4 gram bacteria were used to describe the in vitro bioactivities. The GC-MS analysis showed that the volatile components of MDHW were mainly fatty compounds and terpenoids, with sesquiterpenes and their derivatives dominating the terpene composition. β-elemene was the main terpene component in the steam distillation (11.88%) and ultrasonic extraction (8.2%) methods. A total of 67 compounds, comprising 45 alkaloids, 9 flavonoids, 6 lignans, and others, were found by UPLC-MS analysis. The primary structural kinds of the non-volatile components were 35 isoquinoline alkaloids. Alkaloids were the predominant active constituent in all MDHW extracts, including crude extracts, alkaloid fractions, and non-alkaloid fractions. These extracts all demonstrate some biological effects in terms of antioxidant, enzyme inhibition, and bacterial inhibition. The findings of this study show that MDHW is abundant in chemical structure types, has great bioactivity assessment, and has the potential to be used to create natural antioxidants, products that postpone Alzheimer’s disease and lower blood sugar levels and antibacterial agents.

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Section: Non-volatile Compoundsmentioning

confidence: 99%

A Study of the Chemical Composition and Biological Activity of Michelia macclurei Dandy Heartwood: New Sources of Natural Antioxidants, Enzyme Inhibitors and Bacterial Inhibitors

Chen

Wei

2023

IJMS

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“…Even without consideration of the low identical efficiency, the reagent consumption and time cost are significant. The high-performance liquid chromatography (HPLC) and related tandem techniques were used as improved approaches [16,17]. Among all kinds of hyphenated techniques [18,19], HPLC coupled with mass spectrometry (LC-MS) provides a great improvement in specificity, sensitivity, and quantification [20].…”

Section: Introductionmentioning

confidence: 99%

Screening of Alkaloids in Sophora Flavescens with Liquid Chromatography Tandem Data-Dependent Acquisition Mass Spectrometry

Wang,

Ding

2023

J. Pharm. Res. Int.

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A novel and simple hyphenated method was applied for the screening of alkaloids in S. flavescens. The approach combined easy sample extraction with an ultra-high-performance liquid chromatography (UPLC) and a high-resolution mass spectrometry (HRMS) in the data-dependent acquisition (DDA) method. With the optimized chromatographic program, ten alkaloids were screened. Among them, matrine, oxymatrine, oxysophoridine, sophocarpine, and oxysophocarpine were identified and quantified. The other five alkaloids were identified with accurate m/z value in MS1 and product ions in MS2. The quantification method was validated with specificity, linearity, recovery, and precision. The LOD and LOQ were determined. In twelve batches of collected S. flavescens roots, the identified ten alkaloids were all observed. The oxymatrine was the most abundant component, and sophocarpine was the least abundant in these samples. This comprehensive method is a powerful tool for the biochemical and clinical research of S. flavescens. It can also be adopted in resource utilization and related medicine quality control.

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Extraction of Phytoconstituents for Lifestyle Diseases

Tchamgoue,

Ngandjui,

Talla

et al. 2023

Role of Herbal Medicines

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Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

Cited by 11 publications

References 23 publications

A Study of the Chemical Composition and Biological Activity of Michelia macclurei Dandy Heartwood: New Sources of Natural Antioxidants, Enzyme Inhibitors and Bacterial Inhibitors

A Study of the Chemical Composition and Biological Activity of Michelia macclurei Dandy Heartwood: New Sources of Natural Antioxidants, Enzyme Inhibitors and Bacterial Inhibitors

Screening of Alkaloids in Sophora Flavescens with Liquid Chromatography Tandem Data-Dependent Acquisition Mass Spectrometry

Extraction of Phytoconstituents for Lifestyle Diseases

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