Systematic Study of Enzymatic Degradation and Plasmid DNA Complexation of Mucus Penetrating Star‐Shaped Lysine/Sarcosine Polypept(o)ides with Different Block Arrangements

Abstract

Systematic study of enzymatic degradation and plasmid DNA complexation Systematic study of enzymatic degradation and plasmid DNA complexation of mucus penetrating star-shaped lysine/sarcosine polypept(o)ides with of mucus penetrating star-shaped lysine/sarcosine polypept(o)ides with different block arrangements. different block arrangements.

Star-shaped poly(l-lysine) with polyester bis-MPA dendritic core as potential degradable nano vectors for gene delivery

“…In a recent study we have demonstrated the degradability of the PLL arms on PPI 8-arm star polypeptide by the proteolytic enzyme trypsin. 42 In light of that, here we exclusively focused on investigating hydrolytic degradation of the bis-MPA core. Previously reported results of degradation studies on hydroxy terminated bis-MPA dendrimers showed fast degradation at physiological and basic pH with a first degradation fraction at pH 7.5 appearing after only 6 h. 25 All degradation studies were standardised and carried out on G3-(32)-PLL 10 in aqueous media at a concentration 0.3 mM for 6 days at 37 °C.…”

Enhanced rate of sarcosine N-carboxyanhydride polymerisation from a lysine dendrimer macroinitiator driven by neighbouring H-bonding effects

“…20 So far, little is understood about the effect of neighbouring groups on the polymerisation of PSar from macroinitiators, despite other examples where PSar has been polymerised from dendrimer initiators previously. 4,[21][22][23] Monitoring the reaction kinetics of NCA polymerisations can be challenging owing to the instability of NCA monomers and therefore any offline reaction monitoring must be done quickly under inert conditions, e.g. in a glovebox, or quenched prior to analysis to have an accurate representation of the reaction e.g.…”

Synthesis and Structure Optimization of Star Copolymers as Tunable Macromolecular Carriers for Minimal Immunogen Vaccine Delivery

“…As an alternative, fully synthetic star copolymer-based vaccines comprising minimal immunogens linked to hydrophilic polymer arms radiating from dendrimer cores provide the potential advantages that minimal immunogen density, orientation, and scaffold size can be precisely tuned to modulate antibody responses. Earlier work has established the utility of star copolymers as tunable platforms for a range of medicinal applications including delivery of chemotherapeutics for cancer treatment as well as prevention or treatment of genitourinary tract infections, providing proof of concept for use in humans. − More recently, star copolymer-based minimal immunogen vaccines were shown to be safe for administration to mice and primates for inducing neutralizing antibody responses; however, the impact of various structural parameters (minimal immunogen density and hydrodynamic diameter) on antibody responses and the synthetic approach to optimize such parameters needs further investigation . Indeed, ligand density, linker length, and hydrodynamic size have been shown to impact antibody responses with other platforms. − Furthermore, optimizing these parameters for glycosylated minimal immunogens, which are notoriously poorly immunogenic may be even more critical to ensuring effective antibody responses …”