Systematic study of the stress-responsive Rboh gene family in Nicotiana tabacum: Genome-wide identification, evolution and role in disease resistance

Yu, Shizhou; Yang, Zhixiao; Nawaz, Zarqa; Lin, Shaobin; Guo, Yushuang; Ren, Xueliang; Baloch, Akram Ali; Han, Dejun

doi:10.1016/j.ygeno.2019.08.010

Cited by 32 publications

(34 citation statements)

References 65 publications

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“…In the Arabidopsis genome, 10 RBOH homologous genes ( AtRBOH A-J ) have been annotated [ 35 ]. Different RBOH copy numbers exist in other plant species, including six in barley [ 36 ] and 20 in Nicotiana tabacum [ 37 ]. Differentially expressed in different plant tissues, RBOH -mediated ROS burst significantly affects plant physiological processes such as pollen tube growth and root hair cell expansion; and plant defenses against biotic and abiotic stresses, including heavy metals [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological and transcriptomic analyses reveal ascorbate and glutathione coregulation of cadmium toxicity resistance in wheat genotypes

et al. 2021

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Background Cadmium (Cd) is a heavy metal with high toxicity that severely inhibits wheat growth and development. Cd easily accumulates in wheat kernels and enters the human food chain. Genetic variation in the resistance to Cd toxicity found in wheat genotypes emphasizes the complex response architecture. Understanding the Cd resistance mechanisms is crucial for combating Cd phytotoxicity and meeting the increasing daily food demand. Results Using two wheat genotypes (Cd resistant and sensitive genotypes T207 and S276, respectively) with differing root growth responses to Cd, we conducted comparative physiological and transcriptomic analyses and exogenous application tests to evaluate Cd detoxification mechanisms. S276 accumulated more H2O2, O2−, and MDA than T207 under Cd toxicity. Catalase activity and levels of ascorbic acid (AsA) and glutathione (GSH) were greater, whereas superoxide dismutase (SOD) and peroxidase (POD) activities were lower in T207 than in S276. Transcriptomic analysis showed that the expression of RBOHA, RBOHC, and RBOHE was significantly increased under Cd toxicity, and two-thirds (22 genes) of the differentially expressed RBOH genes had higher expression levels in S276 than inT207. Cd toxicity reshaped the transcriptional profiling of the genes involving the AsA-GSH cycle, and a larger proportion (74.25%) of the corresponding differentially expressed genes showed higher expression in T207 than S276. The combined exogenous application of AsA and GSH alleviated Cd toxicity by scavenging excess ROS and coordinately promoting root length and branching, especially in S276. Conclusions The results indicated that the ROS homeostasis plays a key role in differential Cd resistance in wheat genotypes, and the AsA-GSH cycle fundamentally and vigorously influences wheat defense against Cd toxicity, providing insight into the physiological and transcriptional mechanisms underlying Cd detoxification.

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Section: Discussionmentioning

confidence: 99%

Comparative physiological and transcriptomic analyses reveal ascorbate and glutathione coregulation of cadmium toxicity resistance in wheat genotypes

et al. 2021

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“…The first plant Rboh gene was found in rice, named OsRbohA, for which the mammalian homolog is gp91 phox /Nox2 (Keller et al, 1998). Then more Rbohs from plants were successively identified from model plants (including Arabidopsis and tobacco) and crops, such as tomato, potato, and cucumber (Torres et al, 1998;Yoshioka et al, 2003;Sagi et al, 2004;Kobayashi et al, 2006;Xia et al, 2009;Yu et al, 2019). Generally, plant Rboh proteins are best described as integral plasma membrane proteins containing six transmembrane (TM) domains.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Respiratory Burst Oxidase Homologs (Rboh) Gene Family and Function of GbRboh5/18 on Verticillium Wilt Resistance in Gossypium barbadense

Chang

Shi

et al. 2020

Front. Genet.

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Respiratory burst oxidase homologs (Rbohs) play a predominant role in reactive oxygen species (ROS) production, which is crucial in plant growth, differentiation, as well as their responses to biotic and abiotic stresses. To date, however, there is little knowledge about the function of cotton Rboh genes. Here, we identified a total of 87 Rbohs from five sequenced Gossypium species (the diploids Gossypium arboreum, Gossypium raimondii, and Gossypium australe, and the allotetraploids Gossypium hirsutum and Gossypium barbadense) via BLAST searching their genomes. Phylogenetic analysis of the putative 87 cotton Rbohs revealed that they were divided into seven clades. All members within the same clade are generally similar to each other in terms of gene structure and conserved domain arrangement. In G. barbadense, the expression levels of GbRbohs in the CladeD were induced in response to a fungal pathogen and to hormones (i.e., jasmonic acid and abscisic acid), based upon which the main functional member in CladeD was discerned to be GbRboh5/18. Further functional and physiological analyses showed that the knock-down of GbRboh5/18 expression attenuates plant resistance to Verticillium dahliae infection. Combined with the molecular and biochemical analyses, we found less ROS accumulation in GbRboh5/18-VIGS plants than in control plants after V. dahliae infection. Overexpression of GbRboh5/18 in G. barbadense resulted in more ROS accumulation than in control plants. These results suggest that GbRboh5/18 enhances the cotton plants' resistance against V. dahliae by elevating the levels of ROS accumulation. By integrating phylogenetic, molecular, and biochemical approaches, this comprehensive study provides a detailed overview of the number, phylogeny, and evolution of the Rboh gene family from five sequenced

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“…Different RBOH copy numbers exist in other plant species, including six in barley [31] and twenty in Nicotiana tabacum [32]. Differentially expressed in different plant tissues, RBOH-mediated ROS burst signi cantly affect plant physiological processes such as pollen tube growth and root hair cell expansion; and plant defense against biotic and abiotic stresses, including heavy metals [33,34].…”

Section: Discussionmentioning

confidence: 99%

Comparative Physiological and Transcriptomic Analyses Reveal Ascorbate and Glutathione Coregulation of Cadmium Toxicity Resistance in Wheat Genotypes

Zhang

Xiao

Zhao

et al. 2021

Preprint

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Background: Understanding the cadmium (Cd) resistance mechanism is crucial for combating the phytotoxicity of Cd and meeting the increasing food demand daily. A classic symptom of Cd toxicity is root growth inhibition. Results: Using two wheat genotypes (Cd tolerant genotype T207 and Cd sensitive genotype S276) with differing root growths in response to Cd, we conducted comparative physiological and transcriptomic analyses and exogenous application tests to interpret Cd detoxification mechanisms. S276 accumulated more H2O2, O2-, and malonaldehyde than T207. Catalase activity and levels of ascorbic acid (AsA) and glutathione (GSH) were higher, whereas superoxide dismutase and peroxidase activities were lower in T207 than in S276. Transcriptome analysis showed that the expression of RBOHA, RBOHC, and RBOHE significantly increased, whereas that of RBOHB markedly decreased by Cd treatment. The transcriptional levels of 22 genes encoding RBOH were higher, and that of 11 genes were lower in T207 than in S276. The transcription of genes involved in the AsA-GSH cycle was profoundly reshaped by Cd treatment; 124 genes were higher and 43 genes were lower in T207 than in S276. Exogenous combined application of AsA and GSH alleviated Cd toxicity by scavenging excess ROS and coordinately modulating root length and branching, especially in S276.Conclusions: These results indicate that the AsA-GSH cycle fundamentally and vigorously influences plant defense against Cd toxicity, which provides valuable information for further clarification of the mechanisms underlying Cd detoxification.

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Systematic study of the stress-responsive Rboh gene family in Nicotiana tabacum: Genome-wide identification, evolution and role in disease resistance

Cited by 32 publications

References 65 publications

Comparative physiological and transcriptomic analyses reveal ascorbate and glutathione coregulation of cadmium toxicity resistance in wheat genotypes

Comparative physiological and transcriptomic analyses reveal ascorbate and glutathione coregulation of cadmium toxicity resistance in wheat genotypes

Comprehensive Analysis of Respiratory Burst Oxidase Homologs (Rboh) Gene Family and Function of GbRboh5/18 on Verticillium Wilt Resistance in Gossypium barbadense

Comparative Physiological and Transcriptomic Analyses Reveal Ascorbate and Glutathione Coregulation of Cadmium Toxicity Resistance in Wheat Genotypes

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