Systematic transcriptome-wide analysis of mRNA–miRNA interactions reveals the involvement of miR-142-5p and its target (FOXO3) in skeletal muscle growth in chickens

Li, Zhenhui; Abdalla, Bahareldin Ali; Zheng, Ming; He, Xiaomei; Cai, Bolin; Han, Peigong; Ouyang, Hongjia; Chen, Biao; Nie, Qinghua; Zhang, Xiquan

doi:10.1007/s00438-017-1364-7

Cited by 20 publications

(15 citation statements)

References 31 publications

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“…MTT. The results showed that the cell activity was reduced significantly after 4 h of hypoxia, and MiRNAs, known as small non-coding RNAs, play vital roles in many biological processes, regulate gene expressions at post-transcriptional level and interfere negatively with translation and RNA silencing [18]. Previous study [19] shows that miR-142-3p targets box 1, attenuating apoptosis of cardiomyocytes induced by H/R.…”

Section: Discussionmentioning

confidence: 92%

Withdrawn: MicroRNA‐142‐5p inhibits autophagy in cardiomyocytes in a mouse model of hypoxia/reoxygenation

2021

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Cardiomyocyte hypoxia-and ischemia-induced apoptosis is a key challenge in the treatment of cardiovascular diseases, and it has been previously reported that high glucose-induced cardiomyocyte apoptosis can be prevented by autophagy activation. Furthermore, down-regulation of the microRNA miR-142-5p protects cardiomyocytes against hypoxia. However, the effects of miR-142-5p on autophagy of myocardial cells under hypoxia/reoxygenation (H/R) are less well understood. Here, we observed that the optimal time for hypoxia and re-oxygenation was 4 hours (h) and 8 h in our model. Overexpression of miR-142-5p in a cardiomyocyte model of H/R enhanced apoptosis and increased the expression of interleukin-1β (IL-1β), cleaved-caspase 3 and p62, but inhibited autophagy and decreased expression of Beclin-1, protein 1 light chain 3B (LC3B) and B cell leukemia/lymphoma 2 (Bcl-2) at the mRNA and protein level. Silencing of miR-142-5p had the opposite effects. We identified Beclin-1 as a target gene for miR-142-5p. Down-regulation of Beclin-1 inhibited autophagy, with down-regulation of LC3B and up-regulation of p62, but promoted apoptosis in the H/R model; down-regulation of miR-142-5p promoted autophagy and inhibited apoptosis in cardiomyocytes through up-regulation of BECN1. Our results provide new insights that may facilitate the development of treatments for ischemic heart disease.

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Section: Discussionmentioning

confidence: 92%

Withdrawn: MicroRNA‐142‐5p inhibits autophagy in cardiomyocytes in a mouse model of hypoxia/reoxygenation

2021

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“…Of particular interest was the observation that the binding of miR-498 to FOXO3 3′-untranslated region increased its expression levels, an effect that is rare but not without precedent 77, 78 . Finally, overexpression of miR-142-5p in chicken primary myoblasts 79 and miR-155-5p in human foreskin fibroblasts 80 increased cell proliferation. This effect was mediated via a decrease in FOXO3 in both cell types.…”

Section: Microrna-mediated Regulation Of Foxo3mentioning

confidence: 93%

Recent advances in understanding the role of FOXO3

et al. 2018

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The forkhead box O3 (FOXO3, or FKHRL1) protein is a member of the FOXO subclass of transcription factors. FOXO proteins were originally identified as regulators of insulin-related genes; however, they are now established regulators of genes involved in vital biological processes, including substrate metabolism, protein turnover, cell survival, and cell death. FOXO3 is one of the rare genes that have been consistently linked to longevity in in vivo models. This review provides an update of the most recent research pertaining to the role of FOXO3 in (i) the regulation of protein turnover in skeletal muscle, the largest protein pool of the body, and (ii) the genetic basis of longevity. Finally, it examines (iii) the role of microRNAs in the regulation of FOXO3 and its impact on the regulation of the cell cycle.

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“…Thus, changes in the expression of interrelated miRNAs and target genes play a decisive role in controlling age-related alterations in muscle mass and function. In recent years, Illumina sequencing technology has been increasingly used to identify potential key interactions between miRNAs and mRNAs in the development of skeletal muscle [23][24][25]. However, miRNA-mRNA regulatory networks associated with the muscle growth, development and aging of sika deer have not been studied.…”

Section: Introductionmentioning

confidence: 99%

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

Jia

Liu

et al. 2020

Genes

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Studies of the gene and miRNA expression profiles associated with the postnatal late growth, development, and aging of skeletal muscle are lacking in sika deer. To understand the molecular mechanisms of the growth and development of sika deer skeletal muscle, we used de novo RNA sequencing (RNA-seq) and microRNA sequencing (miRNA-seq) analyses to determine the differentially expressed (DE) unigenes and miRNAs from skeletal muscle tissues at 1, 3, 5, and 10 years in sika deer. A total of 51,716 unigenes, 171 known miRNAs, and 60 novel miRNAs were identified based on four mRNA and small RNA libraries. A total of 2,044 unigenes and 11 miRNAs were differentially expressed between adolescence and juvenile sika deer, 1,946 unigenes and 4 miRNAs were differentially expressed between adult and adolescent sika deer, and 2,209 unigenes and 1 miRNAs were differentially expressed between aged and adult sika deer. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that DE unigenes and miRNA were mainly related to energy and substance metabolism, processes that are closely associate with the growth, development, and aging of skeletal muscle. We also constructed mRNA–mRNA and miRNA–mRNA interaction networks related to the growth, development, and aging of skeletal muscle. The results show that mRNA (Myh1, Myh2, Myh7, ACTN3, etc.) and miRNAs (miR-133a, miR-133c, miR-192, miR-151-3p, etc.) may play important roles in muscle growth and development, and mRNA (WWP1, DEK, UCP3, FUS, etc.) and miRNAs (miR-17-5p, miR-378b, miR-199a-5p, miR-7, etc.) may have key roles in muscle aging. In this study, we determined the dynamic miRNA and unigenes transcriptome in muscle tissue for the first time in sika deer. The age-dependent miRNAs and unigenes identified will offer insights into the molecular mechanism underlying muscle development, growth, and maintenance and will also provide valuable information for sika deer genetic breeding.

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Systematic transcriptome-wide analysis of mRNA–miRNA interactions reveals the involvement of miR-142-5p and its target (FOXO3) in skeletal muscle growth in chickens

Cited by 20 publications

References 31 publications

Withdrawn: MicroRNA‐142‐5p inhibits autophagy in cardiomyocytes in a mouse model of hypoxia/reoxygenation

Withdrawn: MicroRNA‐142‐5p inhibits autophagy in cardiomyocytes in a mouse model of hypoxia/reoxygenation

Recent advances in understanding the role of FOXO3

Altered miRNA and mRNA Expression in Sika Deer Skeletal Muscle with Age

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