Systematically integrated metabonomic-proteomic studies of Escherichia coli under ciprofloxacin stress

Li, Wanxin; Zhang, Song; Wang, Xiaoyun; Yu, Jing; Li, Zeqi; Lin, Wenxiong; Lin, Xiangmin

doi:10.1016/j.jprot.2018.03.002

Cited by 39 publications

(21 citation statements)

References 47 publications

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“…Amino acids, referred to as the building blocks of proteins, are compounds that play many critical roles in cells. Various proteomics and transcriptomics studies revealed that biosynthesis of amino acids is changed dramatically under antibiotic stress (Le et al, 2016;Li et al, 2018;Maass et al, 2018;Zampieri et al, 2017). In particular, Zampieri et al showed that amino acid biosynthesis was the most affected pathway in E. coli in the early phase of antibiotic stress (Zampieri et al, 2017).…”

Section: Biosynthesis Of Amino Acids and Ribosomal Proteinsmentioning

confidence: 99%

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Integrative proteomics and metabolomics approach to elucidate the antimicrobial effect of simvastatin on Escherichia coli

Koçak¹,

Nemutlu

Kır

et al. 2021

Biomedical Chromatography

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Globally, simvastatin is one of the most commonly used statin drugs. Its antimicrobial properties have been investigated against various pathogens. However, its effect on biological processes in bacteria has been unclear. This study focused on altered biological and metabolic processes at protein and metabolite levels induced by simvastatin. MS-based proteomics and metabolomics were used to investigate the altered proteins and metabolites between experimental groups. Proteomics results showed that simvastatin induced various antimicrobial targets such as chaperon protein DnaK and cell division protein FtsZ. Metabolomics results revealed phenotypic changes in cells under simvastatin stress. Integrated proteomics and metabolomics result indicated that various metabolic processes were altered to adapt to stress conditions. Energy metabolism (glycolysis, tricarboxylic acid cycle, etc.), amino acid synthesis and ribosomal proteins, and purine and pyrimidine synthesis were induced by the effect of simvastatin. This study will contribute to the understanding of antimicrobial properties of statin drugs.

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Section: Biosynthesis Of Amino Acids and Ribosomal Proteinsmentioning

confidence: 99%

“…Metabolomics has been used to clear the adaptation and resistance mechanism of pathogens under different conditions. The integration of proteomics and metabolomics data provided essential information on the alteration of phenotype of pathogens under antibiotic stress conditions (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Integrative proteomics and metabolomics approach to elucidate the antimicrobial effect of simvastatin on Escherichia coli

Koçak¹,

Nemutlu

Kır

et al. 2021

Biomedical Chromatography

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“…Las respuestas bacterianas a nivel molecular en presencia de algún efector de su crecimiento llevan a cambios en los niveles de expresión de proteínas que son responsables de diversos procesos fisiológicos como la transducción de señales celulares, reacciones bioquímicas de catálisis [10], entre otros [11]. Por lo anterior, el objetivo de este trabajo fue realizar una aproximación inicial al conocimiento en los cambios proteómicos ocasionados por Ib-M1 e IONP@Ib-M1 sobre Escherichia coli O157:H7.…”

Section: Introductionunclassified

“…50, no. 1, pp [3][4][5][6][7][8][9][10][11][12]. 2021 Cambios en el perfil protéico de E. coli O157:H7 frente al tratamiento con Ib-M1 e IONP@Ib-M1…”

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Cambios en el perfil proteico de E. coli O157:H7 frente al tratamiento con Ib-M1 e IONP@Ib-M1

et al. 2021

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Escherichia coli O157:H7 es una bacteria patógena responsable de enfermedades transmitidas por alimentos y reconocida por su capacidad de resistencia a diversos antibióticos; razón por la cual, se generan complicaciones en el tratamiento de infecciones producidas por esta bacteria. El péptido Ib-M1 libre e inmovilizado en nanopartículas magnéticas de óxido de hierro (IONP@Ib-M1) ha surgido como una nueva alternativa antimicrobiana contra E. coli O157:H7 y aislados clínicos de esta bacteria. El mecanismo de acción de Ib-M1 e IONP@Ib-M1 contra E. coli O157:H7 aún es desconocido; por lo tanto, el objetivo de esta investigación fue la identificación del cambio en el perfil de proteínas de E. coli O157:H7 luego del tratamiento con Ib-M1 e IONP@Ib-M1 como primer paso para determinar su mecanismo de acción. Para esto, se llevó a cabo la obtención de proteínas, posteriormente se llevó a cabo una electroforesis bidimensional para finalmente realizar la determinación de la variabilidad de los perfiles proteicos. Una vez obtenidos los perfiles proteicos se llevó a cabo un análisis de varianza (ANOVA). Los resultados muestran la identificación de proteínas expresadas diferencialmente y las cuales se encuentra involucradas en procesos de quimiotaxis, síntesis y mantenimiento de proteínas, procesos redox, crecimiento celular, transporte de aminoácidos e inhibición de traducción.

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“…Transcriptional sequencing technology has facilitated the development of veterinary molecular biology thus it has also become the frontier of biological and medical research [17][18][19]. Next-generation sequencing could illuminate the overall transcriptional activities of a given species at the single nucleotide level [20].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiles of Murine Bone Marrow-Derived Dendritic Cells in Response to Peste des Petits Ruminants Virus

Liú

et al. 2019

Veterinary Sciences

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Background: Peste des petits ruminants virus (PPRV) is the causative agent of PPR, which can cause an acute, highly contagious and fatal disease of sheep and goats, resulting in significant economic losses for commercial animal husbandry due to its high mortality and morbidity. As professional antigen-presenting cells, dendritic cells (DCs) play a unique role in innate immunity. This study aimed to gain a deeper understanding of the transcriptional response of bone marrow-derived dendritic cells (BMDCs) stimulated with PPRV. Results: Transcriptional profiling was performed using RNA sequencing. Herein, we reported that compared to untreatedBMDCs, 4492 differentially expressed genes (DEGs) were identified following PPRV stimulation, out of these DEGs 2311 were upregulated and 2181 were downregulated, respectively. A total of three gene ontology (GO) term clusters of biological process, cell component and molecular function were significantly enriched in 963 GO terms in the PPRV-stimulated BMDCs. These GO clusters were related to inflammatory response, cell division and vacuole, anchoring junction, positive regulation of cellular component and nucleoside binding. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of DEGs were enriched in a chemokine signaling pathway, protein processing in endoplasmic reticulum, cell cycle and mTOR signaling pathway. Additionally, identified DEGs of BMDCs were further validated by qRT-PCR and the results were in accordance with the change of the genes. This study suggested the effects of PPRV stimulation on the maturation and function of BMDCs. Conclusion: We found that the dramatic BMDCs transcriptome changes triggered were predominantly related to an inflammatory response and chemokine signaling pathway.

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Systematically integrated metabonomic-proteomic studies of Escherichia coli under ciprofloxacin stress

Cited by 39 publications

References 47 publications

Integrative proteomics and metabolomics approach to elucidate the antimicrobial effect of simvastatin on Escherichia coli

Integrative proteomics and metabolomics approach to elucidate the antimicrobial effect of simvastatin on Escherichia coli

Cambios en el perfil proteico de E. coli O157:H7 frente al tratamiento con Ib-M1 e IONP@Ib-M1

Transcriptional Profiles of Murine Bone Marrow-Derived Dendritic Cells in Response to Peste des Petits Ruminants Virus

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