Systematics of the genus <i>Halimeda</i> (Bryopsidales, Chlorophyta) in Brazil including the description of <i>Halimeda jolyana sp. nov</i>.

Ximenes, Caroline Feijão; Cassano, Valéria; Oliveira-Carvalho, Maria de Fátima de; Bandeira-Pedrosa, Maria Elizabeth; Gurgel, Carlos Frederico D.; Verbruggen, Heroen; Pereira, Sônia Maria Barreto

doi:10.2216/16-77.1

Cited by 9 publications

(10 citation statements)

References 34 publications

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“…However, the range of intraspecific divergence value for tufA observed in this study for Brazilian samples (0-1.57 %) is relatively within the range of variation found for other Bryopsidalean genera, such as Halimeda (0-1.4 %) (Ximenes et al 2019). The interspecific divergence observed for tufA (3.3-16.2 %) is relatively within the range reported for Halimeda, 0.9-12.4 % by Ximenes et al (2017) and 2.0-6.2 % by Ximenes et al (2019), as well as for Codium (Bryopsidales), 1.44-14.65 %, and Ulva (Ulvales), 0.91-9.1 % reported by Saunders & Kucera (2010) Intraspecific divergence within Bryopsis for rbcL (0-0.4 %) was lower than that observed for Halimeda (0.8-1.2 %) by Ximenes et al (2019), whereas the range of interspecific variation for this marker (6.7-11.6 %) is within the range reported for Halimeda, 0.8-11.7 % by Ximenes et al (2017) and 4.5-5.0 % by Ximenes et al (2019). For the psbB marker, Tufiño-Velázquez & Pedroche (2019) proposed a genetic distance range between 5 % to 12 % for species discrimination and, therefore, considered a distance of up to 4.9 % as intraspecific, a higher value than that obtained for tufA and rbcL in this study.…”

Section: Discussionsupporting

confidence: 79%

“…TufA marker (elongation factor Tu gene) obtained the best results in the universality of the primers and quality of the sequences, being proposed as a DNA barcode for green algae, and as a phylogenetic marker as well (Saunders & Kucera 2010). Its effectiveness and successfully as DNA barcode for green algae, showing good phylogenetic resolution at generic and specific level having proven by Dijoux et al (2012), Famà et al (2002), Ximenes et al (2017;, followed by the rbcL (Rubisco large subunit gene) also considered suitable for phylogenetic reconstruction , having been demonstrated a sufficient level of variation to be informative in intergeneric, inter-and intraspecific studies for green and red algae as well (Freshwater & Rueness 1994;Oliveira-Carvalho et al 2012;Leliaert et al 2014;Ximenes et al 2017;. Both have been widely used for other genera in the Bryopsidales, such as Caulerpa, Codium, and Halimeda (Lam & Zechman 2006;Oliveira-Carvalho et al 2012;Kazi et al 2013;Belton et al 2014;Ximenes et al 2017;, and also for other green algae (Mccourt et al 2000;Shimada et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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First molecular analysis of the genus Bryopsis (Bryopsidales, Chlorophyta) from Brazil, with an emphasis on the Pernambuco coast

et al. 2021

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The green algal genus Bryopsis has simple morphology and high phenotypic plasticity, making it difficult to identify its species based on morphological characteristics alone. This study evaluated the diversity of Bryopsis in northeastern Brazil (the State of Pernambuco), based on morphological and molecular data using the markers tufA and rbcL. Molecular analyses were incongruent with morphology, demonstrating the existence of cryptic and polymorphic species in the genus. Of the four taxa cited for the area based only on morphological data (Bryopsis corymbosa, B. pennata, B. plumosa, Bryopsis sp.), only B. pennata was recorded. Typical specimens of B. pennata and B. plumosa were grouped with low genetic divergence, 0-0.21 % for tufA and no divergence for rbcL, indicating that B. pennata is an extremely plastic species that includes specimens with morphotype B. "plumosa". Bryopsis pennata var. secunda is cited for the first time for northeastern Brazil, with divergence from the typical variety of 0.96-1.57 % for tufA and 0.4 % for rbcL. This study showed that broader sampling of Bryopsis is necessary in order to confirm the taxonomic status of the species referenced for Brazil, whose phenotypic plasticity may cause overestimation of diversity or reveal cryptic species.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

First molecular analysis of the genus Bryopsis (Bryopsidales, Chlorophyta) from Brazil, with an emphasis on the Pernambuco coast

et al. 2021

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“…Lateral fusion of the surface utricles has been observed in some taxa belonging to the same section of the genus Halimeda as the studied species [42][43][44]. In such cases, two or more adjacent utricles were fused, which, in the surface view, can be recognized by occurrence of a large and usually distinctly elongated polygon [1].…”

Section: Discussionmentioning

confidence: 68%

Morphometric Analysis of Surface Utricles in Halimeda tuna (Bryopsidales, Ulvophyceae) Reveals Variation in Their Size and Symmetry within Individual Segments

Neustupa

Němcová

2020

Symmetry

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Calcifying marine green algae of genus Halimeda have siphonous thalli composed of repeated segments. Their outer surface is formed by laterally appressed peripheral utricles which often form a honeycomb structure, typically with varying degrees of asymmetry in the individual polygons. This study is focused on a morphometric analysis of the size and symmetry of these polygons in Mediterranean H. tuna. Asymmetry of surface utricles is studied using a continuous symmetry measure quantifying the deviation of polygons from perfect symmetry. In addition, the segment shapes are also captured by geometric morphometrics and compared to the utricle parameters. The area of surface utricles is proved to be strongly related to their position on segments, where utricles near the segment bases are considerably smaller than those located near the apical and lateral margins. Interestingly, this gradient is most pronounced in relatively large reniform segments. The polygons are most symmetric in the central parts of segments, with asymmetry uniformly increasing towards the segment margins. Mean utricle asymmetry is found to be unrelated to segment shapes. Systematic differences in utricle size across different positions might be related to morphogenetic patterns of segment development, and may also indicate possible small-scale variations in CaCO3 content within segments.

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“…To better solve this taxonomic issue, we envisage that the multilocus or genomewide data from an expanded specimen sampling could help to fill this taxonomic knowledge gap. Despite the issue of incomplete lineage sorting, echoed by the view by Dijoux et al (2012), Pongparadon et al (2015), and Ximenes et al (2017), we found that tufA remains the most suitable marker for assessing the biodiversity of Halimeda because this marker allows us to quickly identify Halimeda species in that it contains a rich sequence dataset and provides a good phylogenetic resolution at the generic and specific levels.…”

Section: Discussionmentioning

confidence: 82%

“…These variations and influences pose a great challenge to marine biologists striving to differentiate these species solely based on the external morphologies. The genus Halimeda contains cryptic and pseudocryptic species that can lead to erroneous identifications (Kooistra et al 2002; Verbruggen et al 2006; Pongparadon et al 2015; Cremen et al 2016; Ximenes et al 2017). For example, many species represent criptic diversity and are part of species complexes such as Halimeda cuneata and Halimeda discoidea (Cremen et al 2016; Ximenes et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Species diversity and distribution of the calcareous green macroalgae Halimeda in Taiwan, Spratly Island, and Dongsha Atoll, with the proposal of Halimeda taiwanensis sp. nov.

Phetcharat

Pattarach

Chen

et al. 2023

Phycological Research

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The calcified green algal genus Halimeda is one of the most ecologically important but morphologically diverse seaweeds in sub-tropical and tropical waters. Because of its high morphological plasticity, the identification of Halimeda species based on morphological characters is challenging without the assistance of molecular analysis. To date, the species diversity of Halimeda in Taiwan and its overseas territories has not been investigated with the assistance of DNA sequencing, and this taxonomic knowledge gap should be filled. The present study initiates a systematic examination of the species diversity and distribution of Halimeda in Taiwan, Spratly Island, and Dongsha Atoll in the South China Sea, using DNA sequence data (plastid tufA gene and rbcL) and morphological data. Our DNA analyses revealed the presence of 10 Halimeda species (Halimeda borneensis, Halimeda cylindracea, Halimeda discoidea, Halimeda distorta, Halimeda macroloba, Halimeda minima, Halimeda opuntia, Halimeda renschii, Halimeda taiwanensis sp. nov., and Halimeda velasquezii) in the waters around Taiwan, Spratly Island, and Dongsha Atoll. The majority of the species could be readily distinguished by their morphological and anatomical characters. The proposed new species, H. taiwanensis, was differentiated not only by our algorithmic species delimitation analyses (statistical parsimony network analysis and automated barcode gap discovery), but also by its morphological features. The proposed new species differs from two externally resembled species, H. cuneata and H. discoidea, in having an undulated segment margin, the complete fusion of medullary siphons at the node, the lack of segment stalk, and the presence of a large primary utricle. Here, we present the up-to-date taxonomic account, molecular diversity, and geographical distribution of Halimeda spp. in Taiwan and associated areas of the South China Sea. Environmental factors that might drive the occurrence and latitudinal distribution of the species are also discussed.

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Systematics of the genus Halimeda (Bryopsidales, Chlorophyta) in Brazil including the description of Halimeda jolyana sp. nov.

Cited by 9 publications

References 34 publications

First molecular analysis of the genus Bryopsis (Bryopsidales, Chlorophyta) from Brazil, with an emphasis on the Pernambuco coast

First molecular analysis of the genus Bryopsis (Bryopsidales, Chlorophyta) from Brazil, with an emphasis on the Pernambuco coast

Morphometric Analysis of Surface Utricles in Halimeda tuna (Bryopsidales, Ulvophyceae) Reveals Variation in Their Size and Symmetry within Individual Segments

Species diversity and distribution of the calcareous green macroalgae Halimeda in Taiwan, Spratly Island, and Dongsha Atoll, with the proposal of Halimeda taiwanensis sp. nov.

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