Systematics of the Trembleya sensu stricto clade of Microlicia (Melastomataceae, Lavoisiereae)

Abstract

A systematic monograph of the Trembleya s.s. clade is presented, a Brazilian endemic lineage of Melastomataceae comprising 11 species and currently recognised as part of Microlicia s.l. (Melastomataceae). First, we investigate phylogenetic relationships within Lavoisiereae using two nuclear markers and two sampling datasets (102 and 134 terminals). Then, we provide a systematic revision and new circumscription of the Trembleya s.s. clade, including line drawings, photos of living specimens, leaves and floral parts, distribution maps, a key to the 11 accepted species, comments on morphology, reproductive biology, richness, endemism, biogeography and recommended conservation assessments. A nomenclatural update of all taxa previously treated in Trembleya is also provided, including the designation of 45 lectotypes and the proposal of 38 new synonyms.

A Phylogenetic Analysis of Marcetia (Melastomataceae, Marcetieae) and Three New Sprawling Species from Bahia, Brazil

“…All markers were amplified in 15 µl volume reactions using 1 µl of template DNA. The PCR recipes and parameters used to amplify each region can be found in the following literature: Pacifico et al (2022), ETS, ITS; Penneys et al (2020), ndhF, psbK-psbL , rbcL ; Versiane et al (2021), trnS-trnG . The amplified fragments were cleaned using 5–7 µl of PCR products and 2 µl of diluted ExoSAP-IT, which were incubated at 37°C for 30 minutes and 80°C for 15 minutes.…”