Systematische Untersuchungen zur Quantifizierung von genomischer DNA aus Hautproben

Abstract

A systematic approach for quantification of genomic DNA from skin samples Abstract In this study we investigated if skin samples ate suitable DNA sources for molecular biological examinations. For this purpose appropriate extraction, detection and quantification methods are required. These demands are met by phenol extraction and by the QuantiBlot Human DNA Quantitation Kit. Skin samples were taken from different regions from corpses of both sexes (face, elbow, knee, sole of the foot, neck and back). The parallel use of two extraction methods and subsequent quantification indicated higher yields of genomic DNA with the phenol-extraction than with Chelex-extraction. The average DNA content from skin traces from the neck. back, sole of the foot and elbow was 690.95 ng/mg dried skin. Paraffin-embedded skin samples (average DNA coetent of ten 5 ~m-thick paraffin sections ranged from 2.5 to 25 ng) are also useful for molecular investigations. All skin samples could be typed with four STR systems (HUM-FES/FPS, HUMTH01, HUMF13A01 and HUMVWA31) and Amelogenin. A hit-and-run offence described in this report could be clarified by means of DNA typing of a skin abrasion from the victim on the vehicle. Key words