Systemic Administrations of Water-Dispersible Single-Walled Carbon Nanotubes: Activation of NOS in Spontaneously Hypertensive Rats

Shapoval, L. M.; Dmytrenko, O. V.; Сагач, В. Ф.; Prylutska, Svitlana; Khrapatiy, S. V.; Zavodovskyi, D. O.; Prylutskyy, Yu. І.; Tsierkezos, Nikos G.; Ritter, Uwe

doi:10.1007/s11062-020-09858-1

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…According to the AFM results, the majority of SWCNTs in water suspension (0.1 mg/mL) appear as aggregates (bundles) with a height of up to 40 nm [5]. However, non-aggregated, i.e., single, SWCNTs with a diameter of 0.5-1.5 nm, which can interact with the TRPC4 channel pore and are consistent with the selected computed molecular models (see below), were also present [19].…”

Section: Preparation and Characterization Of Swcnts Water Suspensionsupporting

confidence: 72%

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Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

et al. 2021

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Single-walled carbon nanotubes (SWCNTs) are characterized by a combination of rather unique physical and chemical properties, which makes them interesting biocompatible nanostructured materials for various applications, including in the biomedical field. SWCNTs are not inert carriers of drug molecules, as they may interact with various biological macromolecules, including ion channels. To investigate the mechanisms of the inhibitory effects of SWCNTs on the muscarinic receptor cation current (mICAT), induced by intracellular GTPγs (200 μM), in isolated mouse ileal myocytes, we have used the patch-clamp method in the whole-cell configuration. Here, we use molecular docking/molecular dynamics simulations and direct patch-clamp recordings of whole-cell currents to show that SWCNTs, purified and functionalized by carboxylation in water suspension containing single SWCNTs with a diameter of 0.5–1.5 nm, can inhibit mICAT, which is mainly carried by TRPC4 cation channels in ileal smooth muscle cells, and is the main regulator of cholinergic excitation–contraction coupling in the small intestinal tract. This inhibition was voltage-independent and associated with a shortening of the mean open time of the channel. These results suggest that SWCNTs cause a direct blockage of the TRPC4 channel and may represent a novel class of TRPC4 modulators.

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Section: Preparation and Characterization Of Swcnts Water Suspensionsupporting

confidence: 72%

“…The toxic effect of SWCNTs on normal cells and living systems is determined by the following factors: chemical composition, size, dose and exposure route [17,18]. Previously, we have reported that the systemic introduction of 0.1 mg/mL carboxylated SWCNTs (diameter of 0.5-1.5 nm) does not have any adverse effects is rats [19].…”

Section: Introductionmentioning

confidence: 99%

Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

et al. 2021

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“…The functionalized SWCNTs were received from Prof. Uwe Ritter, Institut für Chemie und Biotechnik, Technische Universität Ilmenau, Ilmenau, Germany and were suspended in a distilled water, sonicated on ice for 15 minutes, and filtered through membrane filter (pore size was 0.22 μm). These nanoparticles have partially been characterized previously using atomic force microscopy and transmission electron microscopy (Minchenko et al 2018;Shapoval et al 2020). The diameter of SWCNTs is 1-2 nm (atomic force microscopy study) and their length up to 210 nm (determined by pore size of membrane filter).…”

Section: Methodsmentioning

confidence: 99%

The impact of single walled carbon nanotubes on the expression of microRNA in zebrafish (Danio rerio) embryos

Minchenko

Khita

Rudnytska

et al. 2022

Endocrine Regulations

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Objective. Single-walled carbon nanotubes (SWCNTs) are able to cross the blood-brain barrier, penetrate through the cell membrane, and accumulate in the cell nucleus, which purposefully allows their use in the health sciences as imaging probes and drug carriers in the cancer therapy. The aim of this study was to investigate the effect of low doses of SWCNTs on the expression of microRNAs associated with the cell proliferation and the brain development in zebrafish (Danio rerio) embryos. Methods. The zebrafish embryos (72 h post fertilization) were exposed to low doses of SWCNTs (2 and 8 ng/ml of medium) for 24 or 72 h. The microRNAs (miR-19, miR-21, miR-96, miR-143, miR-145, miR-182, and miR-206) expression levels were measured by quantitative polymerase chain reaction analysis. Results. It was found that low doses of SWCNTs elicited dysregulation in the expression of numerous cell proliferation and brain development-related microRNAs (miR-19, miR-21, miR-96, miR-143, miR-145, miR-182, and miR-206) in dose- (2 and 8 ng/ml of medium) as well as malformations in the zebrafish embryos brain development in a time-dependent (24 and 72 h) manner. Conclusion. Taken together, the present data indicate that the low doses of SWCNTs disturbed the genome functions and reduced the miR-19, miR-21, miR-96, miR-143, miR-145, miR-182, and miR-206 expression levels in dose- and time-dependent manners and interrupted the brain development in the zebrafish embryos indicating for both the genotoxic and the neurotoxic interventions.

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Modeling of Single-Walled Carbon Nanotube Binding to Nitric Oxide Synthase and Guanylate Cyclase Molecular Structures

et al. 2020

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Systemic Administrations of Water-Dispersible Single-Walled Carbon Nanotubes: Activation of NOS in Spontaneously Hypertensive Rats

Cited by 4 publications

References 23 publications

Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

Single-Walled Carbon Nanotubes Inhibit TRPC4-Mediated Muscarinic Cation Current in Mouse Ileal Myocytes

The impact of single walled carbon nanotubes on the expression of microRNA in zebrafish (Danio rerio) embryos

Modeling of Single-Walled Carbon Nanotube Binding to Nitric Oxide Synthase and Guanylate Cyclase Molecular Structures

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