Systemic and local eosinophil inflammation during the birch pollen season in allergic patients with predominant rhinitis or asthma

Kämpe, Mary; Stålenheim, Gunnemar; Janson, Christer; Stolt, Ingrid; Carlson, Marie

doi:10.1186/1476-7961-5-4

Cited by 22 publications

(52 citation statements)

References 31 publications

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“…The season visit was made 2-3 weeks after the pollen counts had reached 4,000 grains/m 3 of air, pollen grains counted by the Palynological Laboratory, Swedish Museum of Natural History, Stockholm, Sweden [21]. The study was performed during the birch pollen seasons in 2000 and 2002; the season of 2001 was excluded due to the low pollen counts.…”

Section: Methodsmentioning

confidence: 99%

PI3-Kinase Regulates Eosinophil and Neutrophil Degranulation in Patients with Allergic Rhinitis and Allergic Asthma Irrespective of Allergen Challenge Model

et al. 2011

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The PI3K pathway plays a major role in many vital cell processes. Our primary aim was to investigate signalling through PI3K for in vitro degranulation from allergen-primed eosinophils and neutrophils in allergic rhinitis and allergic asthma after seasonal and experimental allergen challenge. Nine patients with allergic rhinitis, eight with allergic asthma and four controls were studied during birch pollen season and after nasal and bronchial allergen challenge. Primed blood eosinophils and neutrophils were stimulated for in vitro degranulation with C3b-coated Sephadex particles, after prior incubation with Wortmannin, a PI3K inhibitor. The released amounts of eosinophil cationic protein (ECP), eosinophil peroxidase (EPO) and myeloperoxidase (MPO) were measured by radioimmunoassay. Wortmannin (10(-6) to 10(-9) M) inhibited ECP, EPO and MPO release in a dose-dependent manner in allergic rhinitis and allergic asthma in all three allergen challenge models. Inhibition of ECP release tended to be lower in the asthmatics in all allergen challenge models, statistically significant compared to the controls during season for 10(-8) M Wortmannin (p=0.01). A clear propensity towards less inhibition in the rhinitic patients was seen after nasal and bronchial challenge compared to seasonal exposure, significant for ECP (10(-8) M Wortmannin; p=0.034 and 0.002, respectively). Signalling through PI3K is clearly involved in ECP, EPO and MPO release in allergic rhinitis and allergic asthma irrespective of allergen challenge model. Allergic asthma demonstrated less inhibition of ECP release via PI3K during pollen season, indicating that other pathways play a greater role in eosinophil degranulation in allergic asthma than allergic rhinitis.

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Section: Methodsmentioning

confidence: 99%

PI3-Kinase Regulates Eosinophil and Neutrophil Degranulation in Patients with Allergic Rhinitis and Allergic Asthma Irrespective of Allergen Challenge Model

et al. 2011

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“…Similarly, the peak birch pollen season may provoke symptoms at the end of May, when pollen concentrations were decreased to moderate and low levels. Another explanation might be that the severity of bronchial hyperresponsiveness (BHR) in nonasthmatic patients changes with pollen-induced rhinitis during the season and evidence suggests that during the pollen season, and even after the cessation of exposure to pollen, BHR is increased for some weeks (Tilles and Bardana 1997;Kämpe et al 2007). …”

Section: Discussionmentioning

confidence: 99%

Allergy symptoms in relation to alder and birch pollen concentrations in Finland

2011

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In order to study allergic people responding to daily changes in pollen concentrations, we compared personal diary data on allergic symptoms and the use of allergy medicines to daily pollen counts during the two unequal alder and birch pollen seasons of 2009 and 2010. Almost 90% of the 61 subjects with physician-diagnosed birch pollinosis developed conjunctival, nasal or other symptoms during the peak birch pollination. Most subjects (95%) also reported symptoms during the alder pollination. Despite a delay between the most severe symptoms and the pollen peaks and the increased risk of allergy symptoms between the alder and birch pollen peaks at much lower pollen concentrations, the number of subjects with allergy symptoms correlated with the daily pollen concentrations in both years (r 09 = 0.35, r 10 = 0.36, p \ 0.01). The positive correlation was even stronger (r 09 = 0.69, r 10 = 0.74, p \ 0.001) in relation to the cumulative sum of daily concentrations. The use of allergy medicines precisely followed the abundance of allergy symptoms in both years (r 09 = 0.96, r 10 = 0.70, p \ 0.001). We conclude that there is a fair correlation between the daily allergy symptoms and the particular pollen concentrations, but the risk of developing symptoms at low, moderate and high concentrations is affected by the progression of the pollen season.

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“…It has been shown in previous studies that ECP levels in nasal lavage fluid of patients with AR is higher than that of the patients without AR [18]. Moreover, ECP levels were found to change in the pollen season and with immunotherapy in these patients [18, 19]. …”

Section: Discussionmentioning

confidence: 99%

Correlation of Quality of Life with Clinical Parameters and Eosinophilic Cation Protein Levels in Children with Allergic Rhinoconjunctivitis

Yüksel

Yılmaz²,

Söğüt³

et al. 2008

Int Arch Allergy Immunol

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Background: Systemic and mucosal roles of eosinophils in the pathogenesis of allergic rhinoconjunctivitis (AR) are known. The aim of this study was to investigate the relationship of clinical parameters and quality of life with eosinophilic cationic protein (ECP) in grass pollen-sensitive children with seasonal AR. Methods: This study included 31 children with AR and 18 healthy controls aged between 5 and 15 years. ECP levels in nasal lavage fluid and serum were measured. AR symptom scores were calculated and the pediatric rhinoconjunctivitis quality of life questionnaire (PRQLQ) was filled in for all patients. Results: Mean serum and nasal ECP levels (ng/ml) were significantly higher in the patient group (p < 0.05 for both). In the AR group, the mean clinical symptom score was 7.3 ± 2.2, while the total PRQLQ score, activity limitation, symptoms and emotional function domains were 2.5 ± 0.9, 3.0 ± 1.1, 2.5 ± 1.2 and 2.6 ± 1.1, respectively. The total clinical symptom score and disease duration showed a significant correlation with the total PRQLQ score (p = 0.00 and 0.003, respectively). However, neither nasal lavage nor serum ECP levels were significantly correlated with symptom score, duration of disease, PRQLQ total score or domains (p > 0.05 for all). Conclusion: These results may indicate the absence of a correlation between clinical status and quality of life and levels of ECP in tissues with allergic inflammation.

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Systemic and local eosinophil inflammation during the birch pollen season in allergic patients with predominant rhinitis or asthma

Cited by 22 publications

References 31 publications

PI3-Kinase Regulates Eosinophil and Neutrophil Degranulation in Patients with Allergic Rhinitis and Allergic Asthma Irrespective of Allergen Challenge Model

PI3-Kinase Regulates Eosinophil and Neutrophil Degranulation in Patients with Allergic Rhinitis and Allergic Asthma Irrespective of Allergen Challenge Model

Allergy symptoms in relation to alder and birch pollen concentrations in Finland

Correlation of Quality of Life with Clinical Parameters and Eosinophilic Cation Protein Levels in Children with Allergic Rhinoconjunctivitis

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