The kinetics and mechanism of complex formation between chromium (III) and diethylenetriaminepentaacetic acid (DTPA) were studied spectrophotometrically in an aqueous acid medium over the temperature range of 35-50C using large excess of the ligand. The reaction was found to be firstorder in chromium (III) concentration and zero-order in DTPA concentration. The rate of reaction showed inverse dependence on hydrogen ion concentration. Values of 104 kJ mol -1 and 14 JK -1 mol -1 were obtained for enthalpy and entropy of activations, respectively. I d mechanism has been proposed based upon the release of water molecule from the active species [Cr(H 2 O) 5 OH] 2+ in a rate-determining step. The rate law has been established to be: rate =