Systemic CA-MRSA infection following trauma during soccer match in inner Brazil: clinical and molecular characterization

Camargo, Carlos Henrique; Cunha, Maria de Lourdes Ribeiro de Souza da; Bonesso, Mariana Fávero; Cunha, Fabiana Picoli da; Barbosa, Alexandre Naime; Fortaleza, Carlos Magno Castelo Branco

doi:10.1016/j.diagmicrobio.2013.03.013

Cited by 17 publications

(19 citation statements)

References 18 publications

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“…It is worth reporting that one adolescent with CA-MRSA cellulitis that started after trauma in a soccer match and progressed to severe sepsis was diagnosed in Bofete, a small town neighboring Botucatu. The isolate belonged to ST5, spa type 311, and harbored PVL genes [71]. As with our results, this occurrence demonstrates the circulation of CA-MRSA in regions distant from major urban centers, and reinforces the necessity of including CA-MRSA in the agenda of public health offices in Brazil.…”

Section: Discussionsupporting

confidence: 87%

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

et al. 2014

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Recent increases in the incidence and severity of staphylococcal infections renewed interest in studies that assess the burden of asymptomatic carriage of Staphylococcus aureus in the community setting. We conducted a population-based survey in the city of Botucatu, Brazil (122,000 inhabitants), in order to identify the prevalence of nasal carriage of Staphylococcus aureus (including methicillin-resistant strains). Nasal swabs were obtained from 686 persons over one year of age. Resistance to methicillin was assessed through phenotypic methods, identification of the mecA gene and typing of the Staphylococcal Chromosome Cassette mec (SCCmec). Methicillin-resistant S. aureus (MRSA) isolates were characterized using Pulsed-Field Gel Electrophoresis (PFGE), Multilocus Sequence Typing (MLST) and spa typing. Polymerase chain reaction was applied to identify genes coding for Panton-Valentine Leukocidin (PVL) in isolates. The prevalence of overall S. aureus carriage was 32.7% (95%CI, 29.2%–36.2%). Carriers were significantly younger (mean age, 28.1 versus 36.3 for non-carriers; OR for age, 0.98; 95%CI, 0.97–0.99) and likely to report recent skin infection (OR, 1.85; 95%CI, 1.03–3.34). Carriage of methicillin-resistant S. aureus (MRSA) was found in 0.9% of study subjects (95%CI, 0.4%–1.8%). All MRSA isolates harbored SCCmec type IV, and belonged to spa types t002 or t021, but none among them harbored genes coding for PLV. In MLST, most isolates belonged to clones ST5 or ST1776. However, we found one subject who carried a novel clone, ST2594. Two out of six MRSA carriers had household contacts colonized with isolates similar to theirs. Our study pointed to dissemination of community-associated MRSA among the Brazilian population.

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Section: Discussionsupporting

confidence: 87%

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

et al. 2014

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“…MRSA type IV, ST-5/CC5 was also isolated from blood cultures and tracheal aspirate of a young patient from a small town near Botucatu (Bofete, about 9000 inhabitants) who had no history of exposure in the health care setting or recent travel. The patient had a trauma after a soccer game that progressed to cellulitis, local abscess, pneumonia and severe sepsis [24]. Taken together, these reports demonstrate a predominance of ST5 and CC5 in Botucatu and the surrounding region.…”

Section: Discussionmentioning

confidence: 69%

Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system

Pereira-Franchi

Barreira

Costa

et al. 2019

Tropical Med Int Health

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Objective To evaluate the molecular epidemiology and to georeference Staphylococcus aureus isolated from wounds and nares of patients seen at Basic Health Units (BHUs) of a Brazilian city. Methods Observational, cross‐sectional study conducted from 2010 to 2013. A total of 119 S. aureus strains isolated from the wounds and nares of 88 patients were studied. The isolates were characterised by identifying virulence genes encoding enterotoxins A–E, haemolysins α, β and δ, exfoliatins A, B and D, biofilm production, Panton‐Valentine Leukocidin and toxic shock syndrome toxin 1, and by pulsed‐field gel electrophoresis (PFGE), multilocus sequence and spa typing. Results Eighteen methicillin‐resistant Staphylococcus aureus (MRSA) (6 SCCmec type II and 12 SCCmec type IV) and 101 (85%) MSSA were identified. PFGE typing resulted in the formation of eight clusters, with STs 1, 5, 8, 30, 188, 1176 and 1635 and spa type t002 being the predominant types among MSSA. The 18 MRSA belonged to STs 5, 8 and 1176 and spa types t002 and t062. Conclusion The results demonstrate widespread dissemination of MSSA and MRSA clones carrying haemolysin, biofilm and toxin genes. Kernel density estimation revealed the highest density of S. aureus in the 4, 5 and 8 BHUs.

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“…ST5-II was prevalent in 2009 the Chinese cities of Shenyang and Dalian (Liu et al, 2009). ST5-II/t311 has been found in many other countries, such as Brazil, Nigerian, Angola, Argentina, and Oman (Camargo et al, 2013; Kolawole et al, 2013; Conceição et al, 2014; Egea et al, 2014; Udo et al, 2014). From 2010 to 2014, ST5-II/t311 has surpassed ST5-II/t002 as the predominant clone in our hospital; however, its mechanism remains to be further elucidated.…”

Section: Discussionmentioning

confidence: 98%

Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China

Kong

Zhang

et al. 2017

Front. Microbiol.

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Analysis of the genotypic characteristics and antimicrobial susceptibility patterns of methicillin-resistant Staphylococcus aureus (MRSA) is essential for the control and treatment of diseases caused by this important pathogen. In this study, MRSA isolates obtained from a tertiary caret hospital in China were subjected to spa typing, SCCmec typing, multiple locus sequence typing (MLST), and PCR targeting of the genes encoding Panton-Valentine leukocidin (PVL). The disk diffusion method was used to test the antimicrobial susceptibility of the isolates to 10 non-beta-lactam antibiotics. Among the 120 MRSA isolates studied, 18 spa types and 15 ST types were identified. The spa t311 type was the most common (a total of 60 isolates; 50%) among the study strains, and nearly all the t311 strains belonged to ST5, which is the most common ST type that was previously reported from China among the t002 isolates. ST5-II/t311 was the major prevalent clone (55, 45.8%), which was followed by ST5-II/t002 (12, 10.0%) and ST59-IV/t437 (11, 9.2%). PVL-encoding genes were found in 6.7% of the isolates. Although the ST5-II/t311 and ST5-II/t002 clones are different spa types, they shared the same resistance profile (clindamycin, erythromycin, and ciprofloxacin). Most isolates of the ST239-III/t037 clone were resistant to clindamycin, erythromycin, ciprofloxacin, gentamicin, tetracycline, and trimethoprim/sulfamethoxazole. By contrast, the MRSA isolates of the ST239-III/t030 clone were more resistant to rifampin, but they were susceptible to trimethoprim/sulfamethoxazole. Our data emphasize the need for ongoing epidemiologic surveillance.

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Systemic CA-MRSA infection following trauma during soccer match in inner Brazil: clinical and molecular characterization

Cited by 17 publications

References 18 publications

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

Molecular epidemiology of methicillin‐resistant Staphylococcus aureus in the Brazilian primary health care system

Molecular Epidemiology and Antibiotic Resistance Profiles of Methicillin-Resistant Staphylococcus aureus Strains in a Tertiary Hospital in China

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