Systemic delivery of full-length C/EBPβ/liposome complex suppresses growth of human colon cancer in nude mice

Abstract

C/EBPβ (CCAAT/enhancer-binding protein β) is an important transcription factor involved in cellular proliferation and differentiation. Overexpression of the full-length C/EBPβ protein results in cellular growth arrest and apoptosis. Using a nonviral liposome as carrier, we delivered the full-length C/EBPβ expression plasmid, pCN, into nude mice bearing CW-2 human colon cancer tumors via tail vein. Southern blots revealed that the major organs and tumors were transfected. Experimental gene therapy showed that a strong suppression of tumor growth was observed in the pCNtreated mice, and such suppression was due to the overexpression of C/EBPβ, leading to the increased apoptosis in tumors of pCN-treated mice. No apparent toxic effects of pCN/liposome complex were observed in the animals. Thus, C/EBPβ has tumor suppression effect in vivo and may be used in gene therapy for cancers.

miR-191 promotes tumorigenesis of human colorectal cancer through targeting C/EBPβ

“…Seven candidate targets of miR-191 (unpublished and we are doing further research) were screened. C/EBPβ was chosen for further analysis, because previous reports have shown that C/EBPβ is an important regulator of cell growth [ 26 , 27 ], cell apoptosis [ 28 ] and tumorigenicity [ 29 ]. miR-191 has conserved binding sites in the 3′UTRs of C/EBPβ of different species (Figure 5A ).…”

Decreased brain damage and curtailed inflammation in transcription factor CCAAT/enhancer binding protein β knockout mice following transient focal cerebral ischemia

“…2004). Overexpression of C/EBPβ induces apoptosis leading to suppression of colonic tumor growth in mice (Sun et al . 2005).…”

“…C/EBPb is also known to be involved in apoptosis (Mo et al 2004). Overexpression of C/EBPb induces apoptosis leading to suppression of colonic tumor growth in mice (Sun et al 2005). CHOP is a C/EBP homologous transcription factor (Yoshida et al 2005) and its activation induces astrocytic death in vitro following oxygen-glucose deprivation (Benavides et al 2005).…”

The NF‐κB target genes ICAM‐1 and VCAM‐1 are differentially regulated during spontaneous differentiation of Caco‐2 cells

“…C/EBPβ has been shown to be functional in the differentiation of adipocytes [18], uterine stromal cells [19], mammary epithelial cells [20] and hepatocytes [21]. Additionally, it was also shown that delivery of the full‐length C/EBPβ gene in nude mice suppressed the growth of colonic tumors by inducing apoptosis [22].…”