Systemic Expression of Genes Involved in the Plant Defense Response Induced by Wounding in Senna tora

Kang, Ji-Nam; Lee, Woo-Haeng; Won, So Youn; Chang, Saemin; Hong, Jaemin; Oh, Tae‐Jin; Lee, Si Myung; Kang, Sang-Ho

doi:10.3390/ijms221810073

Cited by 14 publications

(10 citation statements)

References 79 publications

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“…PIs and CHIs both belong to pathogenesis-related (PR) proteins [50]. Proteinase inhibitors (PIs) families are a large and complex group of plant proteins and have the ability to form complexes with a widely accepted five classes of proteolytic enzymes, including serine, cysteine, threonine, aspartic and metallo-proteases, which suppress the normal assimilation of food proteins and bring the adverse effects for insects [51].…”

Section: Expression Analysis Of Degs Involved In Plant-pathogen Inter...mentioning

confidence: 99%

Comparative Transcriptome Analysis of Defense Response of Potato to Phthorimaea operculella Infestation

Zhu,

Yi,

Yang

et al. 2023

Plants

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The potato tuber moth (PTM), Phthorimaea operculella Zeller (Lepidoptera: Gelechiidae), is one of the most destructive pests of potato crops worldwide. Although it has been reported how potatoes integrate the early responses to various PTM herbivory stimuli by accumulatively adding the components, the broad-scale defense signaling network of potato to single stimuli at multiple time points are unclear. Therefore, we compared three potato transcriptional profiles of undamaged plants, mechanically damaged plants and PTM-feeding plants at 3 h, 48 h, and 96 h, and further analyzed the gene expression patterns of a multitude of insect resistance-related signaling pathways, including phytohormones, reactive oxygen species, secondary metabolites, transcription factors, MAPK cascades, plant–pathogen interactions, protease inhibitors, chitinase, and lectins, etc. in the potato under mechanical damage and PTM infestation. Our results suggested that the potato transcriptome showed significant responses to mechanical damage and potato tuber moth infestation, respectively. The potato transcriptome responses modulated over time and were higher at 96 than at 48 h, so transcriptional changes in later stages of PTM infestation may underlie the potato recovery response. Although the transcriptional profiles of mechanically damaged and PTM-infested plants overlap extensively in multiple signaling pathways, some genes are uniquely induced or repressed. True herbivore feeding induced more and stronger gene expression compared to mechanical damage. In addition, we identified 2976, 1499, and 117 genes that only appeared in M-vs-P comparison groups by comparing the transcriptomes of PTM-damaged and mechanically damaged potatoes at 3 h, 48 h, and 96 h, respectively, and these genes deserve further study in the future. This transcriptomic dataset further enhances the understanding of the interactions between potato and potato tuber moth, enriches the molecular resources in this research area and paves the way for breeding insect-resistant potatoes.

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Section: Expression Analysis Of Degs Involved In Plant-pathogen Inter...mentioning

confidence: 99%

Comparative Transcriptome Analysis of Defense Response of Potato to Phthorimaea operculella Infestation

Zhu,

Yi,

Yang

et al. 2023

Plants

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“…Ectopic expression of prosystemin triggers the constitutive expression of PI genes in the absence of wounding, and the increased PI levels are reduced in JA-deficient mutants ( Sun et al, 2011 ). In addition to PIs , expression of PATHOGENESIS-RELATED ( PR ) genes, encoding PR-1, PR-10, chitinase, thaumatin-like protein, and plant defensin, is induced in systemic tissues to enhance plant survival and resistance against further herbivore attack ( Kang et al, 2021 ).…”

Section: Responses To Woundingmentioning

confidence: 99%

Wound-Induced Systemic Responses and Their Coordination by Electrical Signals

Lee

Seo

2022

Front. Plant Sci.

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Wounding not only induces the expression of damage-responsive genes, but also initiates physiological changes, such as tissue repair, vascular reconnection, and de novo organogenesis in locally damaged tissues. Wound-induced signals also propagate from the site of wounding to distal organs to elicit a systemic response. Electrical signaling, which is the most conserved type of systemic signaling in eukaryotes, is triggered by wound-induced membrane potential changes. Changes in membrane potential spread toward systemic tissues in synergy with chemical and hydraulic signals. Here, we review current knowledge on wound-induced local and systemic responses in plants. We focus particularly on how wound-activated plasma membrane-localized ion channels and pumps propagate systemic information about wounding to induce downstream molecular responses in distal tissues. Finally, we propose future studies that could lead to a better understanding of plant electrical signals and their role in physiological responses to wounding.

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“…In our study, the transcript abundances of many genes coding JAZ proteins, such as TIFY7, TIFY9, TIFY10A, and TIFY11B, were increased under mechanical wounding in A. sinensis (Table S3). A gene coding for a JAZ protein in Senna tora, which is highly similar to TIFY10A of A. sinensis, was found to repress JA-regulated responses under mechanical wounding [9]. Further, GH3 (glycoside hydrolase 3) belongs to the auxin-responsive gene families and is tightly associated with the hormone-homeostasis and signaling pathways [51].…”

Section: Characterization Of Genes Related To Plant Hormones Under Me...mentioning

confidence: 99%

“…An understanding of the transcriptome changes during mechanical wounding could contribute to artificially inducing the production of secondary metabolites. One of the important steps in the process of repair from mechanical wounding is the reprogramming of the transcriptome, and it involves several genes including those encoding TFs such as WRKY and AP2 [8][9][10][11]. The 'repair' genes encode proteins that function in signaling, phytohormone regulation, transcription regulation, and the synthesis of secondary metabolites [8].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis Reveals Gene Expression Changes during Repair from Mechanical Wounding in Aquilaria sinensis

Zhuo

et al. 2022

Forests

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Plants repair their mechanical wounds by reprogramming secondary metabolism. However, which genes are reprogrammed during this repair process in Aquilaria sinensis has rarely been studied. Here, we used high-throughput RNA sequencing to explore the changes in the transcriptome of Aquilaria’s xylem, six months after the stem was subjected to mechanical wounding. In total, 1165 transcripts were differentially accumulated, of which 1002 transcripts were increased and 163 were decreased in their abundances (|log2 (fold change)| ≥ 1 and FDR ≤ 0.05). The majority of these genes encode products involved in plant secondary metabolism, transcription regulation, and phytohormone metabolism and signaling. The up-regulated genes were classified into 15 significantly enriched GO terms and were involved in 83 pathways, whereas the down-regulated genes were classified into 5 significantly enriched GO terms and represented 43 pathways. Gene annotation demonstrated that 100 transcripts could encode transcription factors (TFs), such as WRKY, AP2, MYB, and Helix-loop-helix (HLH) TFs. We inferred that the differential expression of TFs, genes associated with plant hormones, phenylpropanoid biosynthesis, and sesquiterpenoid biosynthesis may contribute to the repair of the stem after mechanical wounding in A. sinensis. Using co-expression analysis and prediction of TF binding sites, a TF–gene regulatory network for Aquilaria lignin biosynthesis was constructed. This included the MYB, HLH, WRKY, and AP2 TFs, and the COMT1, 4CLL7, and CCR1 genes. The changes in 10 candidate genes were validated by quantitative reverse-transcription PCR, indicating significant differences between the treated and untreated areas. Our study provides global gene expression patterns under mechanical wounding and would be valuable to further studies on the molecular mechanisms of plant repair in A. sinensis.

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Systemic Expression of Genes Involved in the Plant Defense Response Induced by Wounding in Senna tora

Cited by 14 publications

References 79 publications

Comparative Transcriptome Analysis of Defense Response of Potato to Phthorimaea operculella Infestation

Comparative Transcriptome Analysis of Defense Response of Potato to Phthorimaea operculella Infestation

Wound-Induced Systemic Responses and Their Coordination by Electrical Signals

Transcriptome Analysis Reveals Gene Expression Changes during Repair from Mechanical Wounding in Aquilaria sinensis

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