Systemic hyperthermia in the treatment of HIV-related Kaposi's sarcoma. A phase I study

Alonso, Kenneth; Pontiggia, P; Nardi, C; Sabato, Alessandro; Curto, F Cuppone

doi:10.1016/0753-3322(92)90065-f

Cited by 11 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In both cases, HIV-1 was inactiva ted more slowly than in tissue culture medium; however, in activation was complete in both plasma and in Adsol within 24 h. These results indicate, therefore, that the decrease in the rate of HIV-1 inactivation in red cells, as compared to that in tissue culture medium, is probably a consequence of the red cells protecting the virus from inactivation. This finding is consistent with the report of stabilization against thermal inactivation of chikungunya virus by platelets [24], 16 24 0 4 12 20…”

Section: Discussionsupporting

confidence: 83%

See 1 more Smart Citation

Loss of Red Blood Cell Viability Associated with Limited Thermal Inactivation of Extracellular HIV-1

Stromberg¹,

Kuypers²,

Sawyer³

et al. 1994

Vox Sanguinis

View full text Add to dashboard Cite

The effects of incubation at mildly elevated temperatures on HIV-1 inactivation and in vitro red blood cell properties were investigated. Red cells (55% Hct) were leukodepleted (3 log(10)) by filtration, maintained at 45 or 47 °C for 4 or 8 h, and then stored at 4°C. Hemolysis was twice that of controls after 42-day storage for samples treated for 4h at 45°C, and five times larger for samples heated at 47°C. There was also a significant increase in the rate of potassium loss, an early decrease in ATP levels, and an initial drop in pH for samples treated at either temperature. Larger differences were observed for samples exposed to these elevated temperatures for 8 h. Osmotic deformability curves obtained by ektacytometry showed dramatic decreases in red cell deformability at both temperatures and for both time periods. HIV-1 inactivation in red cells treated at 45 °C (approximately 0.25 log(10)/h) was considerably less than that obtained in tissue culture medium (1-2 log(10)/h). Since the decrease in red cell deformability is likely to indicate reduced red cell function and survival, and the rate of HIV-1 inactivation is low, mild heat treatment is not an adequate process for viral inactivation of red cell products.

show abstract

Section: Discussionsupporting

confidence: 83%

“…In addition, the in vivo use of systemic hyperthermia [16] for treatment of HIV-related disease has been described. As early as 1948, Ham et al [17] reported no changes in eryth rocytes heated for 1 h at temperatures up to 46°C, but did observe morphologic changes at temperatures above 48.6°C.…”

Section: Discussionmentioning

confidence: 99%

Loss of Red Blood Cell Viability Associated with Limited Thermal Inactivation of Extracellular HIV-1

Stromberg¹,

Kuypers²,

Sawyer³

et al. 1994

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

“…58 " 63 The ability to manipulate isolated subpopulations by altering their functional responses in the presence of an external stress may be useful in the treatment of many immunological disorders, diseases, or deficiencies and may sustain the longevity of tissue and tissue con-EFFECT OF PHYSICAL STRESS ON IMMUNE FUNCTION structs. In addition, anti-CTLA-4 has been injected into mice to enhance their immune response to rumors.…”

Section: Discussionmentioning

confidence: 99%

Effect of Physical Stress on Immune Function in Cell Lines: B7-2 Molecules Contribute to Augmented Antigen Presentation Induced by Heat Shock

et al. 1998

View full text Add to dashboard Cite

T cell activation occurs following the recognition of MHC II-antigen complexes on antigenpresenting cells (APCs) and in conjunction with binding of costimulatory moleclues such as B7. The heat shock response is characterized by the synthesis of heat shock proteins (Hsp) that can alter normal subcellular regulatory pathways in order to repair stress induced cellular damage. Because Hsps can also contribute to normal cell function, the present studies were initiated to determine whether heat shock could be adapted to alter APC function and thereby increase T cell responses to alloantigen. LB27.4, a B cell APC line, was exposed to hyperthermic or normal temperature conditions and cocultured with the D10.G4 helper T cell line. Our results indicate that heat shock can enhance the ability of APCs to activate T cell function. The costimulatory molecule, B7-2 is responsible, at least in part, for the enhancement of APC function. Increased function occurred concomitantly with synthesis of Hsps. Using a cell line-based model system, these studies suggest one mechanism of immune regulation based on the physiological response to stress, and describe an engineering strategy whereby cellular function can be manipulated by the adaptation of the stress response.

show abstract

“…176 There is limited experience with local application of heat in the treatment of KS, although there was considerable experimentation with systemic hyperthermia after the discovery of HIV and AIDS but before the development of HAART. [177][178][179] The rationale for these studies was based on the contention that HIV is heat-sensitive and that HIV-infected lymphocytes are more heat-sensitive than uninfected cells. 179 Patient's core temperatures were raised to around 408C to 428C and held for 1 hour via extracorporeal perfusion hyperthermia, which heated blood to 498C ex vivo.…”

Section: Thermotherapymentioning

confidence: 99%

Thermotherapy in dermatologic infections

Doherty

Rosen

2010

Journal of the American Academy of Dermatology

View full text Add to dashboard Cite

Systemic hyperthermia in the treatment of HIV-related Kaposi's sarcoma. A phase I study

Cited by 11 publications

References 23 publications

Loss of Red Blood Cell Viability Associated with Limited Thermal Inactivation of Extracellular HIV-1

Loss of Red Blood Cell Viability Associated with Limited Thermal Inactivation of Extracellular HIV-1

Effect of Physical Stress on Immune Function in Cell Lines: B7-2 Molecules Contribute to Augmented Antigen Presentation Induced by Heat Shock

Thermotherapy in dermatologic infections

Contact Info

Product

Resources

About