Systemic localization of seven major types of carbohydrates on cell membranes by dSTORM imaging

Chen, Junling; Gao, Jing; Zhang, Min; Cai, Mingjun; Xu, Hao; Jiang, Junguang; Zhang, Tian; Wang, Hongda

doi:10.1038/srep30247

Cited by 19 publications

(15 citation statements)

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“…The spatial distribution pattern of glycans has also been demonstrated to affect the stability and behavior of cell membranes ( 86 , 87 ). The LEL specific GCs have recently been reported to exist as clusters in a confined region ( 88 ). The region-specific micro-heterogeneity in the abundance of T disaccharide i.e., galactosyl (β-1,3) N-acetylgalactosamine, galactose of the T antigen, and N-acetyl glucosamine (cognate glycans for ABL, JAC and LEL, respectively) observed in the lectin cytochemistry experiments reflect distinctive and spatially distributed, putatively functional micro-domains on the buffalo sperm.…”

Section: Discussionmentioning

confidence: 99%

A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils

et al. 2020

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The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu. The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication. This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls. We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities. Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa. Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa. This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs). The mean percentage of phagocytizing PMNs was significantly different ( P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively. Furthermore, any introduced perturbations in the inherent sperm glycan arrangements promoted phagocytosis by PMNs. For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% ( P < 0.01). Likewise, the MPR increased to 70.63% ( p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls. Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% ( P < 0.0001). This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa. Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.

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Section: Discussionmentioning

confidence: 99%

A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils

et al. 2020

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“…There are many kinds of carbohydrates in plants [40][41][42]. Different kinds of carbohydrates can participate in maintaining stability of cell membrane structure function [43]. However, there are also significant differences in the types of carbohydrates accumulated by different varieties of plants under cold stress.…”

Section: Discussionmentioning

confidence: 99%

Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress

Dong

Jiang

et al. 2020

Preprint

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Background Low temperature limits the growth and geographical distribution of plants. Poa pratensis is a cool-season turfgrass mainly grown in urban areas. However, low winter temperature or cold events in spring and autumn may cause P.pratensis mortality, affecting the appearance of lawns. P.pratensis var. anceps cv. Qinghai (PQ) is widely distributed in the Qinghai-Tibet Plateau above 3000 m. PQ has greater cold resistance than the commercially cultivated P.pratensis varieties. However, existing studies on the response mechanism of PQ to low temperatures have mainly focused on physiological and biochemical perspectives, while changes in the PQ transcriptome during the response to cold stress have not been reported. Results To investigate the molecular mechanism of the PQ cold response and identify genes to improve the low-temperature resistance of P.pratensis, we analyzed and compared the transcriptomes of PQ and the cold-sensitive P.pratensis cv. ‘Baron’ (PB) under cold stress using RNA sequencing. We identified 4878 and 1871 differentially expressed genes (DEGs) between the treatment vs control comparison of PQ and PB, respectively, with 4494 DEGs specific to PQ. Based on the DEGs, important Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as “starch and sucrose metabolism”, “protein processing in endoplasmic reticulum”, “phenylalanine metabolism” and “glycolysis/gluconeogenesis” were significantly enriched in PQ, and “starch and sucrose metabolism”, “phenylpropanoid biosynthesis”, “galactose metabolism” and “glutathione metabolism” were significantly enriched in PB. In addition, the “glycolysis” and “citrate cycle (TCA cycle)” pathways were identified as involved in cold resistance of P.pratensis. Conclusions As we know, this is the first study to explore the transcriptome of P.pratensis var. anceps cv. Qinghai. Our study not noly provides important insights into the molecular mechanisms of P.pratensis var. anceps cv. Qinghai responds to cold stress, but also systematically reveals the changes of key genes and products of glycolysis and TCA cycle in response to cold stress, which is conductive to the breeding of cold-resistant P.pratensis genotype.

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“…Based on the core structures of N‐linked and O‐linked oligosaccharides,13 the common monosaccharide residues are the following ones:13 N‐acetyl‐D‐glucosamine (GlcNAc), N‐acetylgalactosamine (GalNAc), fucose (Fuc), mannose (Man), galactose (Gal), and sialic acid (Sia). Moreover, based on our related studies on carbohydrates6,14, 15, 16 we found the carbohydrates tended to form clusters as functional domains, where various functional glycocongujates locate via being cross‐linked by carbohydrate‐binding proteins (CBPs), with the contributions of lipid rafts as the stable factors and actin cytoskeletion as restricted factors. These findings indicate that the organization of carbohydrates not only represents the distributions of membrane glycoconjugates, but also is related to the membrane functional domains and membrane cytoskeletion.…”

Section: Introductionmentioning

confidence: 93%

Variation in Carbohydrates between Cancer and Normal Cell Membranes Revealed by Super‐Resolution Fluorescence Imaging

et al. 2016

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Carbohydrate alterations on cell membranes are associated with various cancer processes, including tumorigenesis, malignant transformation, and tumor dissemination. However, variations in the distributions of cancer‐associated carbohydrates are unclear at the molecular level. Herein, direct stochastic optical reconstruction microscopy is used to reveal that seven major types of carbohydrates tended to form obvious clusters on cancer cell membranes compared with normal cell membranes (both cultured and primary cells), and most types of carbohydrates present a similar distributed characteristic on various cancer cells (e.g., HeLa and Os‐Rc‐2 cells). Significantly, sialic acid is found to distribute in larger‐sized clusters with a higher cluster coverage percentage on various cancer cells than normal cells. These findings on the aberrant distributions of cancer‐associated carbohydrates can potentially serve as novel diagnostic and therapeutic targets, as well as making a contribution to clarify how abnormal glycosylations of membrane glycoconjugates participate in tumorigenesis and metastasis.

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Systemic localization of seven major types of carbohydrates on cell membranes by dSTORM imaging

Cited by 19 publications

References 50 publications

A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils

A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils

Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress

Variation in Carbohydrates between Cancer and Normal Cell Membranes Revealed by Super‐Resolution Fluorescence Imaging

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