2022
DOI: 10.3791/61504
|View full text |Cite
|
Sign up to set email alerts
|

Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3

Citation Types

0
3
0

Year Published

2022
2022
2024
2024

Publication Types

Select...
2
1

Relationship

1
2

Authors

Journals

citations
Cited by 3 publications
(3 citation statements)
references
References 27 publications
0
3
0
Order By: Relevance
“…Protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher, Waltham, MA). Samples were normalized to a concentration of 0.75 μg in 37.5 μL of Milliplex® MAP Assay Buffer (EMD Millipore, Burlington, MA) because this loading fell within a linear range of detectable analytes 38 . Multiplexed cytokine quantification was conducted using the Milliplex® MAP Mouse Cytokine/Chemokine 32-Plex Kit (Eotaxin*, G-CSF, GM-CSF*, IFN-γ*, IL-1α, IL-1β*, IL-2, IL-3*, IL-4*, IL-5*, IL-6*, IL-7*, IL-9*, IL-10*, IL-12p40*, IL-12p70*, IL-13, IL-15*, IL-17*, IP-10, KC, LIF*, LIX*, MCP-1*, M-CSF*, MIG, MIP-1α, MIP-1β*, MIP-2, RANTES, TNF-α, and VEGF) (EMD Millipore) and read on a MAGPIX® system (Luminex, Austin, TX).…”
Section: Methodsmentioning
confidence: 99%
“…Protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher, Waltham, MA). Samples were normalized to a concentration of 0.75 μg in 37.5 μL of Milliplex® MAP Assay Buffer (EMD Millipore, Burlington, MA) because this loading fell within a linear range of detectable analytes 38 . Multiplexed cytokine quantification was conducted using the Milliplex® MAP Mouse Cytokine/Chemokine 32-Plex Kit (Eotaxin*, G-CSF, GM-CSF*, IFN-γ*, IL-1α, IL-1β*, IL-2, IL-3*, IL-4*, IL-5*, IL-6*, IL-7*, IL-9*, IL-10*, IL-12p40*, IL-12p70*, IL-13, IL-15*, IL-17*, IP-10, KC, LIF*, LIX*, MCP-1*, M-CSF*, MIG, MIP-1α, MIP-1β*, MIP-2, RANTES, TNF-α, and VEGF) (EMD Millipore) and read on a MAGPIX® system (Luminex, Austin, TX).…”
Section: Methodsmentioning
confidence: 99%
“…The average background intensity reading from each cytokine panel was subtracted from the raw cytokine abundance values, and negative values were imputed to zero. To find appropriate loading volume for samples, linear ranging was performed as previously published ( 37 ) and 24% of total sample volume was loaded. Assays were read on a MAGPIX instrument (Luminex).…”
Section: Methodsmentioning
confidence: 99%
“…The average background intensity reading from each cytokine panel was subtracted from the raw cytokine abundance values and negative values were imputed to zero. To find appropriate loading volume for samples, linear ranging was performed as previously published 82 and 24% of total sample volume was loaded. Assays were read on a MAGPIX instrument (Luminex).…”
Section: Methodsmentioning
confidence: 99%