“…Protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher, Waltham, MA). Samples were normalized to a concentration of 0.75 μg in 37.5 μL of Milliplex® MAP Assay Buffer (EMD Millipore, Burlington, MA) because this loading fell within a linear range of detectable analytes 38 . Multiplexed cytokine quantification was conducted using the Milliplex® MAP Mouse Cytokine/Chemokine 32-Plex Kit (Eotaxin*, G-CSF, GM-CSF*, IFN-γ*, IL-1α, IL-1β*, IL-2, IL-3*, IL-4*, IL-5*, IL-6*, IL-7*, IL-9*, IL-10*, IL-12p40*, IL-12p70*, IL-13, IL-15*, IL-17*, IP-10, KC, LIF*, LIX*, MCP-1*, M-CSF*, MIG, MIP-1α, MIP-1β*, MIP-2, RANTES, TNF-α, and VEGF) (EMD Millipore) and read on a MAGPIX® system (Luminex, Austin, TX).…”