“…20,[56][57][58][59] TEA is a computational tool used to estimate the performance and cost of a facility during the design stage. 15,33,71 The primary unit processes for cell rupture for product recovery and conversion include, (i) cell disruption, (ii) protein solubilization, (iii) protein precipitation and purication, and (iv) protein concentration and hydrolysis. 21,72 Chemical composition uncertainty in algae, particularly protein content, result in pretreatment and nal product inconsistencies.…”