Systems and Methods for Studying Microbial Processes and Communities in Landfills

Weaver, Joseph E.; Ling, Wang; ReyesIII, Francis L. de los; Barlaz, Morton A.

doi:10.1007/978-3-030-10777-2_5

Cited by 5 publications

(2 citation statements)

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“…Due to geographical and societal variation, the composition of landfill waste is highly heterogeneous [2], supporting diverse consortia of degradative microorganisms. Cellulose and hemicellulose are the major biodegradable components of MSW and their conversion to biogas in landfills is well documented [3, 4]. The hydrolytic potential of landfill microbiota is also well known [5]; however, the taxonomic and functional diversity, and physiology, of landfill microbiota is still poorly understood [6, 7].…”

Section: Full-textmentioning

confidence: 99%

Lutispora saccharofermentans sp. nov., a mesophilic, non-spore-forming bacterium isolated from a lab-scale methanogenic landfill bioreactor digesting anaerobic sludge, and emendation of the genus Lutispora to include species which are non-spore-forming and mesophilic

Houari

Carpenter

Chaplin

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel anaerobic, mesophilic, non-spore-forming bacterium (strain m25T) was isolated from methanogenic enrichment cultures obtained from a lab-scale methanogenic landfill bioreactor containing anaerobic digester sludge. Cells were Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, and motile by means of a flagellum. The genomic DNA G+C content was 40.11 mol%. The optimal NaCl concentration, temperature and pH for growth were 2.5 g l−1, 35 °C and at pH 7.0, respectively. Strain m25T was able to grow in the absence of yeast extract on glycerol, pyruvate, arginine and cysteine. In the presence of 0.2 % yeast extract, strain m25T grew on carbohydrates and was able to use glucose, cellobiose, fructose, raffinose and galactose. The novel strain could utilize glycerol, urea, pyruvate, peptone and tryptone. The major fatty acids were iso-C15 : 0, C14 : 0, C16 : 0 DMA (dimethyl acetal) and iso-C15 : 0 DMA. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate was closely related to Lutispora thermophila EBR46T (95.02 % 16S rRNA gene sequence similarity). Genome relatedness was determined using both average nucleotide identity and amino acid identity analyses, the results of which both strongly supported that strain m25T belongs to the genus Lutispora . Based on its unique phylogenetic features, strain m25T is considered to represent a novel species within the genus Lutispora . Moreover, based on its unique physiologic features, mainly the lack of spore formation, a proposal to amend the genus Lutispora is also provided to include the non-spore-forming and mesophilic species. Lutispora saccharofermentans sp. nov. is proposed. The type strain of the species is m25T (=DSM 112749T=ATCC TSD-268T).

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Section: Full-textmentioning

confidence: 99%

Lutispora saccharofermentans sp. nov., a mesophilic, non-spore-forming bacterium isolated from a lab-scale methanogenic landfill bioreactor digesting anaerobic sludge, and emendation of the genus Lutispora to include species which are non-spore-forming and mesophilic

Houari

Carpenter

Chaplin

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…Knowing the microbial diversity associated with leachate enables the isolation methodologies for recovery of the speci c microorganisms present in this process, improves knowledge for the bio-augmentation techniques, or environmental enrichment could be developed to metabolizing the toxic compounds present in these environments. Next-generation sequencing made possible whole community characterization in land lls and leachate [5,6]. Although these studies have provided insight into the composition of resident microbial populations, the assessments remain incomplete.…”

Section: Introductionmentioning

confidence: 99%

DNA metabarcoding of the leachate microbiota from sanitary landfill: potential for bioremediation process

et al. 2021

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Microbial pro le knowledge is essential to news alternatives and improvements in current treatments and destinations of land ll leachate that contains a variety of toxic compounds produced by municipal solid waste (MSW) disposal. Environmental DNA metabarcoding is an e cient, quick, and low-cost methodology for whole communities' characterization. In this respect, the leachate from the Sanitary Land ll of Foz do Iguaçu City showed mixed characteristics from both acid and methanogenic phases, and 16S rDNA metabarcoding suggested the dominance of fermenting bacteria belonging to Firmicutes phylum, followed by Proteobacteria, Bacteroidetes and Synergistetes. The leachate acidogenic phase ended up being masked in the chemical and physical analyzes, however was evidenced in the metabarcoding methodology. On the other hand, no speci cally methanogenic group was detected in signi cant abundance. To future application using culturomics approaches to bioremediation the leachate sample presented groups extensively studied, especially the Pseudomonas genus to heavy metals treatments, such as cadmium.

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Urban Soil as a Source and Sink

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2022

Urban Soils

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Systems and Methods for Studying Microbial Processes and Communities in Landfills

Cited by 5 publications

References 81 publications

Lutispora saccharofermentans sp. nov., a mesophilic, non-spore-forming bacterium isolated from a lab-scale methanogenic landfill bioreactor digesting anaerobic sludge, and emendation of the genus Lutispora to include species which are non-spore-forming and mesophilic

Lutispora saccharofermentans sp. nov., a mesophilic, non-spore-forming bacterium isolated from a lab-scale methanogenic landfill bioreactor digesting anaerobic sludge, and emendation of the genus Lutispora to include species which are non-spore-forming and mesophilic

DNA metabarcoding of the leachate microbiota from sanitary landfill: potential for bioremediation process

Urban Soil as a Source and Sink

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