Systems biology of acidophile biofilms for efficient metal extraction

Buetti‐Dinh, Antoine; Herold, Malte; Christel, Stephan; Hajjami, Mohamed El; Bellenberg, Sören; Ilie, Olga; Wilmes, Paul; Poetsch, Ansgar; Sand, Wolfgang; Vera, Mario; Pivkin, Igor V.; Dopson, Mark

doi:10.1038/s41597-020-0519-2

Cited by 9 publications

(7 citation statements)

References 39 publications

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“…The attachment sites on mineral surfaces and how the cells can detect/sense these are still open questions and will need attention in the future. Some authors indicate in their publications (Ohmura et al 1993 ; Sanhueza et al 1999 ; Edwards et al 2001 ; Buetti-Dinh et al 2020 ) that an attachment of cells to metal sulfide surfaces does not occur randomly (Fig. 1 ).…”

Section: Microbe/mineral Interactions—initial Cell Attachmentmentioning

confidence: 99%

“…L. ferriphilum T and L. ferrooxidans possess complete DSF signaling systems, including DSF synthase ( rpfF ), the corresponding two-component signal recognition system, composed of a signal transduction sensor kinase ( rpfC ), and the response regulator ( rpfG ) (Christel et al 2017 ; Bellenberg et al 2018 ). These genes are expressed in L. ferriphilum , and increased rpf gene RNA transcripts are present in continuous iron(II) grown cultures and in batch chalcopyrite cultures (Buetti-Dinh et al 2020 ). Interestingly, DSF synthase showed high expression levels in the axenic planktonic cell subpopulations but also in mixed cultures with S. thermosulfidooxidans T (Bellenberg et al 2018 ).…”

Section: Biofilm Formation and Molecular Controlsmentioning

confidence: 99%

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Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation – part A

Sand

Schippers

Hedrich

et al. 2022

Appl Microbiol Biotechnol

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Bioleaching of metal sulfides is performed by diverse microorganisms. The dissolution of metal sulfides occurs via two chemical pathways, either the thiosulfate or the polysulfide pathway. These are determined by the metal sulfides’ mineralogy and their acid solubility. The microbial cell enables metal sulfide dissolution via oxidation of iron(II) ions and inorganic sulfur compounds. Thereby, the metal sulfide attacking agents iron(III) ions and protons are generated. Cells are active either in a planktonic state or attached to the mineral surface, forming biofilms. This review, as an update of the previous one (Vera et al., 2013a), summarizes some recent discoveries relevant to bioleaching microorganisms, contributing to a better understanding of their lifestyle. These comprise phylogeny, chemical pathways, surface science, biochemistry of iron and sulfur metabolism, anaerobic metabolism, cell–cell communication, molecular biology, and biofilm lifestyle. Recent advances from genetic engineering applied to bioleaching microorganisms will allow in the future to better understand important aspects of their physiology, as well as to open new possibilities for synthetic biology applications of leaching microbial consortia. Key points • Leaching of metal sulfides is strongly enhanced by microorganisms • Biofilm formation and extracellular polymer production influences bioleaching • Cell interactions in mixed bioleaching cultures are key for process optimization

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Section: Microbe/mineral Interactions—initial Cell Attachmentmentioning

confidence: 99%

Section: Biofilm Formation and Molecular Controlsmentioning

confidence: 99%

Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation – part A

Sand

Schippers

Hedrich

et al. 2022

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Noteworthy, this pKa value may be modulated by the choice of the substituents of the ligand framework [62–64] or the metal center [65] In this manner, pKa values close to the physiological pH can be achieved for biological applications. In addition, the specific example reported here could potentially be applied in gastric diseases [66,67] or acidophilic biofilms [36] because it is not only stable under low pH conditions, but activated.…”

Section: Resultsmentioning

confidence: 99%

Enhancing Control Over Nitric Oxide Photorelease via a Molecular Keypad Lock

Elbeheiry,

Schulz

2024

Chemistry A European J

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Based on Boolean logic, molecular keypad locks secure molecular information, typically with an optical output. Here we investigate a rare example of a molecular keypad lock with a chemical output. To this end, the light‐activated release of biologically important nitric oxide from a ruthenium complex is studied, using proton concentration and photon flux as inputs. In a pH‐dependent equilibrium, a nitritoruthenium(II) complex is turned into a nitrosylruthenium(II) complex, which releases nitric oxide under irradiation with visible light. The precise prediction of the output nitric oxide concentration as function of the pH and photon flux is achieved with an artificial intelligence approach, namely the adaptive neuro‐fuzzy inference system. In this manner an exceptionally high level of control over the output concentration is obtained. Moreover, the provided concept to lock a chemical output as well as the output prediction may be applied to other (photo)release schemes.

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“…This is achieved by heterologous expression of proteins of interest, often isolated from bioleaching microorganisms (Gumulya et al 2018 ). Nevertheless, attempts to engineer specific biomining microorganisms (Gumulya et al 2018 ) and biofilms for biomining applications (Buetti-Dinh et al 2020 ) have been performed as well. Deinococcus radiodurans , also known for its astrobiological relevant properties such as resistance to polyextremes and space conditions, has been engineered to enhance its bioremediation capacity (Daly 2000 ).…”

Section: Space Biomining Principlesmentioning

confidence: 99%

The smallest space miners: principles of space biomining

2022

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As we aim to expand human presence in space, we need to find viable approaches to achieve independence from terrestrial resources. Space biomining of the Moon, Mars and asteroids has been indicated as one of the promising approaches to achieve in-situ resource utilization by the main space agencies. Structural and expensive metals, essential mineral nutrients, water, oxygen and volatiles could be potentially extracted from extraterrestrial regolith and rocks using microbial-based biotechnologies. The use of bioleaching microorganisms could also be applied to space bioremediation, recycling of waste and to reinforce regenerative life support systems. However, the science around space biomining is still young. Relevant differences between terrestrial and extraterrestrial conditions exist, including the rock types and ores available for mining, and a direct application of established terrestrial biomining techniques may not be a possibility. It is, therefore, necessary to invest in terrestrial and space-based research of specific methods for space applications to learn the effects of space conditions on biomining and bioremediation, expand our knowledge on organotrophic and community-based bioleaching mechanisms, as well as on anaerobic biomining, and investigate the use of synthetic biology to overcome limitations posed by the space environments.

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Systems biology of acidophile biofilms for efficient metal extraction

Cited by 9 publications

References 39 publications

Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation – part A

Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation – part A

Enhancing Control Over Nitric Oxide Photorelease via a Molecular Keypad Lock

The smallest space miners: principles of space biomining

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