Systems Biology of Monovalent Cation Homeostasis in Yeast

Ariño, Joaquı́n; Aydar, Ebru; Ganser, Daniel; Jorrı́n, Jesús; Kahm, Matthias; Krause, Falko; Petrezsélyová, Silvia; Yenush, Lynne; Zimmermannová, Olga; Heusden, G. Paul H. van; Kschischo, Maik; Ludwig, Jost; Palmer, Christopher P.; Ramos, José; Sychrová, Hana

doi:10.1016/b978-0-12-800143-1.00001-4

Cited by 18 publications

(16 citation statements)

References 133 publications

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“…Signalling through the calcineurin/Crz1 pathway accounts for approximately 50–65% of the total transcriptional response of ENA1 to high pH and salt stresses (Ariño et al, ; Mendizabal, Pascual‐Ahuir, Serrano, & de Larrinoa, ; Petrezsélyová et al, ; Platara et al, ; Serrano et al, ; Yoshimoto et al, ). The ENA1 promoter contains two Crz1‐binding regions at positions −813/−821 and −727/−719, with the downstream element being the most relevant for induction of the gene in response to salt stress and high pH (Mendizabal et al, ; Petrezsélyová et al, ).…”

Section: The Ena Efflux Systemmentioning

confidence: 99%

Monovalent cation transporters at the plasma membrane in yeasts

Ariño

Ramos

Sychrová

2018

Yeast

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Maintenance of proper intracellular concentrations of monovalent cations, mainly sodium and potassium, is a requirement for survival of any cell. In the budding yeast Saccharomyces cerevisiae, monovalent cation homeostasis is determined by the active extrusion of protons through the Pma1 H -ATPase (reviewed in another chapter of this issue), the influx and efflux of these cations through the plasma membrane transporters (reviewed in this chapter), and the sequestration of toxic cations into the vacuoles. Here, we will describe the structure, function, and regulation of the plasma membrane transporters Trk1, Trk2, Tok1, Nha1, and Ena1, which play a key role in maintaining physiological intracellular concentrations of Na , K , and H , both under normal growth conditions and in response to stress.

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Section: The Ena Efflux Systemmentioning

confidence: 99%

Monovalent cation transporters at the plasma membrane in yeasts

Ariño

Ramos

Sychrová

2018

Yeast

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“…Potassium homeostasis is regulated by a complex interplay between transporters and regulatory proteins. Many proteins have been identified that bind to the various S. cerevisiae ion transports [ 8 ] ( http://www.yeastgenome.org ). The function of most of these interactions is still unknown and a role in the regulation of the ion transporters is imaginable.…”

Section: Discussionmentioning

confidence: 99%

“…The yeast Saccharomyces cerevisiae is an excellent model organism to study cation homeostasis (for reviews see: [ 6 – 8 ]). In this yeast intracellular potassium concentration is relatively stable at 200 – 300 mM.…”

Section: Introductionmentioning

confidence: 99%

“…To further understand cation homeostasis in S. cerevisiae a number of European research groups including ours collaborate in the Translucent consortium [ 8 ]. To allow studies on the response of yeast to different concentrations of potassium, a new YNB medium containing negligible concentrations of alkali metal cations has been developed by the consortium [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional response of Saccharomyces cerevisiae to potassium starvation

Anemaet

Heusden

2014

BMC Genomics

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BackgroundIon homeostasis is essential for every cell and aberrant cation homeostasis is related to diseases like Alzheimer’s disease and epilepsy. The mechanisms responsible for cation homeostasis are only partly understood. The yeast Saccharomyces cerevisiae is an excellent organism to study fundamental aspects of cation homeostasis. In this study we investigated the transcriptional response of this yeast to potassium starvation by using Serial Analysis of Gene Expression (SAGE)-tag sequencing.ResultsComparison of transcript levels in cells grown for 60 min in media without potassium with those in cells grown under standard potassium concentrations showed that the mRNA levels of 105 genes were significantly (P < 0.01) up-regulated more than 2.0-fold during potassium starvation and the mRNA levels of 172 genes significantly down-regulated. These genes belong to several functional categories. Genes involved in stress response including HSP30, YRO2 and TPO2 and phosphate metabolism including PHO84, PHO5 and SPL2 were highly up-regulated. Analysis of the promoter of PHO84 encoding a high affinity phosphate transporter, revealed that increased PHO84 RNA levels are caused by both increased Pho4-dependent transcription and decreased RNA turnover. In the latter process antisense transcription may be involved. Many genes involved in cell cycle control, and to a lesser extent genes involved in amino acid transport, were strongly down-regulated.ConclusionsOur study showed that yeast cells respond to potassium starvation in a complex way and reveals a direct link between potassium homeostasis and phosphate metabolism.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-1040) contains supplementary material, which is available to authorized users.

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“…Recently, Huang and Lapitsky have shown that monovalent salt increases colloidal stability when Chitosan-Tripolyphosphate Microgels are forming, and they related this phenomenon with weakening chitosan-Tripolyphosphate binding 10 . It is also well known that in biological systems maintenance of concentration of monovalent salt ions such as K and Na is very important for the cell stability, as the increase or decrease of the concentration of salt can lead to death of a cell 11 .…”

Section: Introductionmentioning

confidence: 99%

Theoretical investigation of a polarizable colloid in the salt medium

Bakhshandeh

2018

Chemical Physics

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Systems Biology of Monovalent Cation Homeostasis in Yeast

Cited by 18 publications

References 133 publications

Monovalent cation transporters at the plasma membrane in yeasts

Monovalent cation transporters at the plasma membrane in yeasts

Transcriptional response of Saccharomyces cerevisiae to potassium starvation

Theoretical investigation of a polarizable colloid in the salt medium

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