Systems infection biology: a compartmentalized immune network of pig spleen challenged with Haemophilus parasuis

Zhao, Ming; Liu, Xiangdong; Li, Xinyun; Chen, Hongbo; Jin, Hui; Zhou, Rui; Zhu, Mengjin; Zhao, Shuhong

doi:10.1186/1471-2164-14-46

Cited by 11 publications

(8 citation statements)

References 48 publications

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“…Clinical signs of this illness are characterized by polyarthritis, fibrinous polyserositis, and meningitis, resulting in serious economic losses in the pork industry throughout the world (Oliveira and Pijoan, 2004 ; Huang et al, 2016 ; Zhang P. et al, 2016 ; Zhou et al, 2016 ). H. parasuis has been a major challenge for the worldwide pork industry (Zhao et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401

Dai

Chang

et al. 2018

Front. Cell. Infect. Microbiol.

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Haemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis. We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus battery of Sxy-dependent cyclic AMP (cAMP) receptor protein (CRP-S) regulons using bioinformatics. High rates of natural competence were found from the onset of stationary-phase growth condition to mid-stationary phase (OD600 from 0.29 to 1.735); this rapidly dropped off as cells reached mid-stationary phase (OD600 from 1.735 to 1.625). As a whole, bacteria cultured in liquid media were observed to have lower competence levels than those grown on solid media plates. We also revealed that natural transformation in this species is stable after 200 passages and is largely dependent on DNA concentration. Transformation competition experiments showed that heterogeneous DNA cannot outcompete intraspecific natural transformation, suggesting an endogenous uptake sequence or other molecular markers may be important in differentiating heterogeneous DNA. We performed qRT-PCR targeting multiple putative competence genes in an effort to compare bacteria pre-cultured in TSB++ vs. TSA++ and SC1401 vs. SH0165 to determine expression profiles of the homologs of competence-genes in H. influenzae. Taken together, this study is the first to investigate natural transformation in H. parasuis based on a highly naturally transformable strain SC1401.

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Section: Introductionmentioning

confidence: 99%

Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401

Dai

Chang

et al. 2018

Front. Cell. Infect. Microbiol.

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“…Analysis of coexpression-type networks has been used previously to identify genes and proteins of increased importance to controlling system-wide host responses [10,[12][13][14][15]. The topological locations of genes in these networks can be used to identify hubs, genes that are connected to many other genes and therefore may be exerting a regulatory influence, and topological bottlenecks, genes that bridge disparate sub-networks and may serve as mediators of transitions between system states [16][17][18]. Recently, a node's participation in network motifs in directional networks was shown to be an effective predictor of importance [19].…”

Section: Introductionmentioning

confidence: 99%

The effect of inhibition of PP1 and TNFα signaling on pathogenesis of SARS coronavirus

et al. 2016

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BackgroundThe complex interplay between viral replication and host immune response during infection remains poorly understood. While many viruses are known to employ anti-immune strategies to facilitate their replication, highly pathogenic virus infections can also cause an excessive immune response that exacerbates, rather than reduces pathogenicity. To investigate this dichotomy in severe acute respiratory syndrome coronavirus (SARS-CoV), we developed a transcriptional network model of SARS-CoV infection in mice and used the model to prioritize candidate regulatory targets for further investigation.ResultsWe validated our predictions in 18 different knockout (KO) mouse strains, showing that network topology provides significant predictive power to identify genes that are important for viral infection. We identified a novel player in the immune response to virus infection, Kepi, an inhibitory subunit of the protein phosphatase 1 (PP1) complex, which protects against SARS-CoV pathogenesis. We also found that receptors for the proinflammatory cytokine tumor necrosis factor alpha (TNFα) promote pathogenesis, presumably through excessive inflammation.ConclusionsThe current study provides validation of network modeling approaches for identifying important players in virus infection pathogenesis, and a step forward in understanding the host response to an important infectious disease. The results presented here suggest the role of Kepi in the host response to SARS-CoV, as well as inflammatory activity driving pathogenesis through TNFα signaling in SARS-CoV infections. Though we have reported the utility of this approach in bacterial and cell culture studies previously, this is the first comprehensive study to confirm that network topology can be used to predict phenotypes in mice with experimental validation.Electronic supplementary materialThe online version of this article (doi:10.1186/s12918-016-0336-6) contains supplementary material, which is available to authorized users.

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“…Of course, the attempted study of some potentially virulence-associated loci is not enough for a deep analysis of the problem, but it does show the need to develop a realistic model of infection based on system biology, genome sequencing and proteomic analysis in which the maximum range of isolates must be involved [23,[28][29][30].…”

Section: Putative Virulence Genes In Studied Haemophilus Parasuis Strmentioning

confidence: 99%

DETERMINATION OF VIRULENCE FACTORS IN Haemophilus parasuis ISOLATES

Павелко¹,

Babin²,

Sprygin³

et al. 2016

S-h. biol.

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A b s t r a c tGlasser's disease is a bacterial infection typically characterized by fibrinous polyserositis, polyarthritis, meningitis, and occasionally acute pneumonia and septicemia. Haemophilus parasuis is the etiologic agent of Glasser's disease, leading to significant economic losses in swine industry. H. parasuis normally colonizes the upper respiratory tract of healthy pigs. Significant variation in virulence, serological and genetic properties has been shown across H. parasuis strains. However, stress, concomitant viral infections, or poor immune status allow H. parasuis to cause disease. The current literature contains a few studies that clarify differences in the genome between highly virulent and avirulent strains in the context of putative virulence factors. The objective of this study was to examine H. parasuis strains isolated on Russian pig farms during the 11-year study period (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011) for the presence of putative virulence loci. We screened 6 strains and 23 field isolates for the presence of 10 virulence factors. Identification of NAD-dependent isolates was performed by biochemical analysis and species-specific real-time PCR. Putative virulence genes were amplified by PCR using different primer pairs. Genetic analysis confirmed that the virulence factors (vtaA, fhuA, hhdA, hhdB, nhaC, HAPS_0254, sclB7, sclB11 and phage_related) exist in different combinations. Out of 28 strains studied, the vtaA gene was found in all strains, sclB7 and sclB11 genes were present in 24 and 25 strains respectively, the cirA gene in 18 out of 28, the fhuA gene in HAPS_0254, the hhdA gеne in 15, the hhdB gene in 14, the nhaC gene in 12, the phage_related target in only 3 strains. Our findings identified 15 novel genotypes (LUB, Ural'skii-DEP, MB, SK-1-DEP, SK3, Krasnodar, Botovo-2, Botovo-5, Botovo-7, IL2, AI4, V171, SH6, SW124, VN). The strains IL-1-DEP, KOMI DEP and isolate Nadeevo-2 carried all the loci, except for phage_related, which has only been reported for the virulent strains IA-84-17975, IA-84-22113 and SD-84-15995. In contrast to the paper by A.V. Potehin et al. (2007), IL-1-DEP turned out to be highly virulent, KOMI DEP exhibited moderately virulence, and Nadeevo-2 showed no virulence at all. Interestingly, the vtaA group 1 gene, the main determinant of virulence, was identified in SK-1-DEP avirulent strain and Botovo-2 and Nadeevo-2 avirulent isolates. This work contributes to a better understanding of putative virulence factors in field isolates of H. parasuis.

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Systems infection biology: a compartmentalized immune network of pig spleen challenged with Haemophilus parasuis

Cited by 11 publications

References 48 publications

Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401

Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401

The effect of inhibition of PP1 and TNFα signaling on pathogenesis of SARS coronavirus

DETERMINATION OF VIRULENCE FACTORS IN Haemophilus parasuis ISOLATES

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