Systems Pharmacological Approach to Investigate the Mechanism of Ohwia caudata for Application to Alzheimer’s Disease

Sun, Yiwei; Wang, Yue; Du, Kaicheng; Meng, Dali

doi:10.3390/molecules24081499

Cited by 15 publications

(16 citation statements)

References 30 publications

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“…O. caudata is a TCM, also called D. caudatum which has been used to treat disorders, such as fever, dysentery, icterohepatitis, and abscess 24,25 . O. caudata also effectively alleviates Alzheimer's disease symptoms through multiple targets in a synergetic manner 50 . Furthermore, Ma et al found that the ethanol extract of O. caudata possesses anti‐inflammatory, antipyretic activities, analgesic, and antipyretic activities and has favorable safety 24 .…”

Section: Discussionmentioning

confidence: 99%

“…24,25 O. caudata also effectively alleviates Alzheimer's disease symptoms through multiple targets in a synergetic manner. 50 Furthermore, Ma et al found that the ethanol extract of O. caudata possesses anti-inflammatory, antipyretic activities, analgesic, and antipyretic activities and has favorable safety. 24 Moreover, Kwon et al suggested that O. caudata extract could protect against influenza A virus infection.…”

Section: Discussionmentioning

confidence: 99%

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Ohwia caudata extract relieves the IL‐17A‐induced inflammatory response of synoviocytes through modulation of SOCS3 and JAK2/STAT3 activation

Kuo

et al. 2023

Environmental Toxicology

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Fibroblast-like synoviocytes accumulation, proliferation and activation, and the subsequent inflammatory mediators production play a key role in the progression of rheumatoid arthritis (RA). It is well established that Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling triggers inflammation, and induces cytokine levels in RA. Ohwia caudata have long been used against many disorders. However, in RA, the effects of O. caudata have not been elucidated. In the current study, synoviocytes were used to evaluate the suppressive effects of O. caudate extract (OCE) on the pro-inflammatory cytokines production. In vitro, the underlying mechanisms by which OCE inhibits inflammatory response through regulation of suppressors of cytokine signaling 3 (SOCS3) and JAK2/STAT3 expression in IL-17A-treated HIG-82 synoviocytes were investigated. The results demonstrated that the proliferation of Tsung-Jung Ho and Chih-Yang Huang contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ohwia caudata extract relieves the IL‐17A‐induced inflammatory response of synoviocytes through modulation of SOCS3 and JAK2/STAT3 activation

Kuo

et al. 2023

Environmental Toxicology

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“…Enrichment Analysis. Similarly, through the DAVID database (https://david.ncifcrf.gov/) for the enrichment analysis of drug-disease intersection protein targets, KEGG pathway enrichment analysis not only provides an annotation of pathway functions for a given gene set but also provides pathway enrichment analysis [27]. For the multiple test correction of the significant P value in enrichment analysis, the enrichment analysis adopts the algorithm designed by the hypergeometric algorithm and uses the Benjamini-Hochberg correction method.…”

Section: Kyoto Encyclopedia Of Genes and Genomes Pathwaymentioning

confidence: 99%

A Network Pharmacology-Based Approach to Investigating the Mechanisms of Fushen Granule Effects on Intestinal Barrier Injury in Chronic Renal Failure

Han

Yang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Purpose. Fushen Granule (FSG) is a Chinese medicine prepared by doctors for treating patients with chronic renal failure, which is usually accompanied by gastrointestinal dysfunction. Here, we explore the protective effect of FSG on intestinal barrier injury in chronic renal failure through bioinformatic analysis and experimental verification. Methods. In this study, information on the components and targets of FSG related to CRF is collected to construct and visualize protein-protein interaction networks and drug-compound-target networks using network pharmacological methods. DAVID is used to conduct gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Then, it is validated by in vitro experiments. In this study, the human intestinal epithelial (T84) cells are used and divided into four groups: control group, model group, FSG low-dose group, and FSG high-dose group. After the experiment, the activity of T84 cells is detected by a MTT assay, and the expressions of tight junction protein ZO-1, claudin-1, nuclear factor erythroid 2-related factor (Nrf2), heme oxygenase-1 (HO-1), malondialdehyde (MDA), and cyclooxygenase-2 (COX-2) are examined by immunofluorescence and/or western blotting. Results. Eighty-six potential chronic renal failure-related targets are identified by FSG; among them, nine core genes are screened. Furthermore, GO enrichment analysis shows that the cancer-related signaling pathway, the PI3K-Akt signaling pathway, the HIF1 signaling pathway, and the TNF signaling pathway may play key roles in the treatment of CRF by FSG. The MTT method showed that FSG is not cytotoxic to uremic toxin-induced injured T84 cells. The results of immunofluorescence and WB indicate that compared with the control group, protein expressions level of ZO-1, claudin-1, and Nrf2 in T84 cells is decreased and protein expressions level of HO-1, MDA, and COX-2 is increased after urinary toxin treatment. Instead, compared with the model group, protein expressions level of ZO-1, claudin-1, and Nrf2 in T84 cells is increased and protein expressions level of HO-1, MDA, and COX-2 is decreased after FSG treatment. Conclusion. FSG had a protective effect on urinary toxin-induced intestinal epithelial barrier injury in chronic renal failure, and its mechanism may be related to the upregulation of Nrf2/HO-1 signal transduction and the inhibition of tissue oxidative stress and inflammatory responses. Screening CRF targets and identifying the corresponding FSG components by network pharmacological methods is a practical strategy to explain the mechanism of FSG in improving gastrointestinal dysfunction in CRF.

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“…GO enrichment analysis illustrates the role of target proteins of TCM compounds in gene function, including the functions of three modules of biological processes, molecular functions, and cellular components [35]. The KEGG pathway enrichment analysis provides not only pathway functional annotations of a given gene set but also the pathway enrichment analysis [36]. Finally, GO and KEGG enrichment analyses were undertaken on the protein of the combined protein in network construction (3).…”

Section: Module Analysismentioning

confidence: 99%

A bioinformatics investigation into the pharmacological mechanisms of the effect of the Yinchenhao decoction on hepatitis C based on network pharmacology

Zhang

Liu

et al. 2020

BMC Complement Med Ther

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Background Globally, more than 170 million people are infected with hepatitis C virus, a major cause of cirrhosis and hepatocellular carcinoma. The Yinchenhao Decoction (YCHD) is a classic formula comprising three herbal medicines. This decoction have long been used in China for clinically treating acute and chronic infectious hepatitis and other liver and gallbladder damp heat-accumulation disorders. Methods In this study, we identified 32 active ingredients and 200 hepatitis C proteins and established a compound-predicted target network and a hepatitis C protein–protein interaction network by using Cytoscape 3.6.1. Then, we systematically analyzed the potential targets of the YCHD for the treatment of hepatitis C. Finally, molecular docking was applied to verify the key targets. In addition, we analyzed the mechanism of action of the predicted targets by the Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses. Results This study adopted a network pharmacology approach, mainly comprising target prediction, network construction, module detection, functional enrichment analysis, and molecular docking to systematically investigate the mechanisms of action of the YCHD in hepatitis C. The targets of the YCHD in the treatment of hepatitis C mainly involved PIK3CG, CASP3, BCL2, CASP8, and MMP1. The module and pathway enrichment analyses showed that the YCHD had the potential to influence varieties of biological pathways, including the TNF signaling pathway, Ras signaling pathway, PI3K-Akt signaling pathway, FoxO signaling pathway, and pathways in cancer, that play an important role in the pathogenesis of hepatitis C. Conclusion The results of this study preliminarily verified the basic pharmacological effects and related mechanisms of the YCHD in the treatment of hepatitis C.

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Systems Pharmacological Approach to Investigate the Mechanism of Ohwia caudata for Application to Alzheimer’s Disease

Cited by 15 publications

References 30 publications

Ohwia caudata extract relieves the IL‐17A‐induced inflammatory response of synoviocytes through modulation of SOCS3 and JAK2/STAT3 activation

Ohwia caudata extract relieves the IL‐17A‐induced inflammatory response of synoviocytes through modulation of SOCS3 and JAK2/STAT3 activation

A Network Pharmacology-Based Approach to Investigating the Mechanisms of Fushen Granule Effects on Intestinal Barrier Injury in Chronic Renal Failure

A bioinformatics investigation into the pharmacological mechanisms of the effect of the Yinchenhao decoction on hepatitis C based on network pharmacology

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