Systems-wide effects of short-term feed deprivation in obese mice

Abstract

Background: While prolonged fasting induces significant metabolic changes in humans and mice, less is known about systems-wide metabolic changes in response to short-term feed deprivation, which traditionally has been used in experimental animal studies prior to metabolic challenge tests. Methods: We here performed a systems biology-based investigation of connections between gut bacterial composition and function, inflammatory and metabolic parameters in the intestine, liver, visceral adipose tissue, blood and urine in obese mice that were feed deprived for varying durations up to 12 hours. Results: Our analysis revealed that increased duration of feed deprivation linked to enhanced intestinal butyric acid production and expression of the gene encoding the pro-thermogenic uncoupling protein UCP1 in visceral adipose tissue of obese mice. Ucp1 expression was also positively associated with Il33 expression in ileum, colon and adipose tissue as well as with the abundance of colonic Porphyromonadaceae, the latter also correlating to cecal butyric acid levels. Conclusions: The data highlighted presence of a three-tiered system of inter-tissue communication involving intestinal, immune and metabolic functions which is affected by the duration of feed deprivation in obese mice.

The Treatment of diarrheal mice with Tenebrio Molitor meal

“…The difference in the liver index of mice may be related to the different dosages of tenebrio molitor meal. With the increase in dosage, fat was d eposited in the liver, increasing liver index [26] . The reason for the difference in spleen and thymus indices of mice among different dosage groups of t enebrio molitor meal may be that the tenebrio molitor meal added to the feed can improve the immunity of mice with diarrhea, and the spleen and thy mus, as part of the immune system, also improve with the increase of tenebrio molitor meal added dose.…”