Syzygium cumini(L.),Skeels fruit extracts: In vitro and in vivo anti-inflammatory properties

Qamar, Muhammad; Akhtar, Saeed; Yuan, Ye; Ahmad, Naveed; Tawab, Abdul; Ismail, Amir; Barnard, Ross; Cooper, Matthew A.; Blaskovich, Mark A. T.; Ziora, Zyta M.

doi:10.1016/j.jep.2021.113805

Cited by 50 publications

(32 citation statements)

References 54 publications

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“…After another centrifugation (2500 rpm; 5 min), absorbance of the reaction mixture was measured at 560 nm using a spectrophotometer; diclofenac sodium was employed as a standard drug and phosphate buffer as the control [ 35 ]. Doses were selected following the recently published research [ 36 ]. The percent inhibition was calculated using the following equation: % inhibition of denaturation = 100 × (A C − A S )/A C where A C = absorption of the control sample, and A S = absorption of the test sample.…”

Section: Methodsmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Effects of Peganum harmala Extracts: An In Vitro and In Vivo Study

et al. 2021

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Peganum harmala (P. harmala) belongs to the family Zygophyllaceae, and is utilized in the traditional medicinal systems of Pakistan, China, Morocco, Algeria, and Spain to treat several chronic health disorders. The aim of the present study was to identify the chemical constituents and to evaluate the antioxidant, anti-inflammatory, and toxicity effects of P. harmala extracts both in vitro and in vivo. Sequential crude extracts including 100% dichloromethane, 100% methanol, and 70% aqueous methanol were obtained and their antioxidant and anti-inflammatory effects evaluated both in vitro and in vivo. The anti-inflammatory effect of the extract was investigated using the carrageenan-induced paw edema method in mice, whereas the toxicity of the most active extract was evaluated using an acute and subacute toxicity rat model. In addition, we have used the bioassay-guided approach to obtain potent fractions, using solvent–solvent partitioning and reversed phase high performance liquid chromatography from active crude extracts; identification and quantification of compounds from the active fractions was achieved using electrospray ionization mass spectrometry and high performance liquid chromatography techniques. Results revealed that the 100% methanol extract of P. harmala exhibits significant in vitro antioxidant activity in DPPH assay with an IC50 of 49 µg/mL as compared to the standard quercetin with an IC50 of 25.4 µg/mL. The same extract exhibited 63.0% inhibition against serum albumin denaturation as compared to 97% inhibition by the standard diclofenac sodium in an in vitro anti-inflammatory assay, and in vivo anti-inflammatory against carrageenan-induced paw edema (75.14% inhibition) as compared to 86.1% inhibition caused by the standard indomethacin. Furthermore, this extract was not toxic during a 14 day trial of acute toxicity when given at a dose of 3 g/kg, indicating that the lethal dose (LD50) of P. harmala methanol extract was greater than 3 g/kg. P. harmala methanolic fraction 2 obtained using bioassay-guided fractionation showed the presence of quinic acid, peganine, harmol, harmaline, and harmine, confirmed by electrospray ionization mass spectrometry and quantified using external standards on high performance liquid chromatography. Taken all together, the current investigation further confirms the antioxidant, anti-inflammatory, and safety aspects of P. harmala, which justifies its use in folk medicine.

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Section: Methodsmentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Effects of Peganum harmala Extracts: An In Vitro and In Vivo Study

et al. 2021

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“…The carrageenan-induced mice paw edema model for determining the anti-inflammatory effect was reported earlier by Qamar et al with small modifications [ 21 ]. Both adult male and female Swiss albino mice in healthy condition, weighed 18–25 g, were used.…”

Section: Methodsmentioning

confidence: 99%

“…Evidence-Based Complementary and Alternative Medicine reported earlier by Qamar et al with small modifications [21]. Both adult male and female Swiss albino mice in healthy condition, weighed 18-25 g, were used.…”

Section: In Vivo Anti-inflammatory Effect Of Oily Capsicummentioning

confidence: 99%

Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications

Nguyen

Truong

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Recently, plant-derived anti-inflammatory products have received an increasing attention from researchers due to their excellent in vivo activity with limited side effects. Therefore, the extraction of natural active compounds from the plant with high purity for use in anti-inflammatory formulations is required. In this study, oily Capsicum oleoresin (OCO) was extracted from Capsicum frutescens L. in ethanol by the ultrasound-assisted extraction technique, followed by a centrifugation step for a high purity OCO extract, which can be applied to develop anti-inflammatory formulations. The impact of various conditions (ethanol concentration, sonicating temperature, extraction time, solvent-to-sample ratio, and extraction repetition) on the efficiency of the extraction process was investigated. The results showed that the optimized conditions for the high yield of OCO were 95% ethanol, 50–60°C, 60 minutes, solvent-to-sample ratio of 5 : 1 ml/g, and one extraction repetition, followed by centrifuging at 5000 rpm in 2 hours. Then, the purity and in vivo anti-inflammatory activities of the obtained OCO was then determined by using the HPLC method and carrageenan-induced mice paw edema model, respectively. The purity of OCO was determined as 3.408 mg capsaicin per gram of Capsicum powder; meanwhile, its anti-inflammatory effect value was approximate to that of the commercial drug diclofenac after 48 hours of treatment. The high purity OCO prepared by this low-cost and ecofriendly extraction process would be a promising material for anti-inflammatory formulations.

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“…The extract was subjected to reverse-phase HPLC-DAD UV/Vis (Agilent LC) for reconfirmation and quantification of tentatively identified bioactive compounds using LC ESI-MS/MS (stigmasterol, quinic acid, malic acid, epicatechin, caffeic acid, rutin, pcoumaric acid, quercetin, ferulic acid, scopoletin, apigenin, and kaempferol) [57]. For separation of the compounds, various chromatographic conditions such as mobile phase, flow rate, detection wavelength, injection volume, and column temperature according to column size C 18 column (SB-C18, 4.6 × 150 mm, 5 µM, Agilent Technologies, Waldbronn Germany) were studied and optimized.…”

Section: Hplc Methods Optimizationmentioning

confidence: 99%

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

et al. 2021

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Cucumis sativus L. is globally cultivated as an edible vegetable. Besides its nutritional benefits, it is used in traditional medicines against various ailments. The current study was designed to elucidate the multi-target mechanisms of a C. sativus seeds extract against asthma and diarrhea using network pharmacology along with a molecular docking approach. Furthermore, in-vitro and in-vivo experiments were conducted to verify the mechanistic insight of in silico studies. LC-ESI-MS/MS was performed to identify the bioactive compounds in the extract; later, some compounds were quantified by HPLC. C. sativus seed. EtOH has kaempferol in higher concentration 783.02 µg/g, followed by quercetin (693.83 µg/g) and luteolin (617.17 µg/g). In silico studies showed that bioactive compounds interfered with asthma and diarrhea-associated target genes, which are members of calcium-mediated signaling to exert a calcium channel blocker activity. The seeds extract exerted a concentration-dependent spasmolytic response on isolated jejunum, trachea, and urinary bladder preparations and caused relaxation of spastic contraction of K+ (80 mM) with suppressed calcium concentration-response curves at dose 0.3 and 1 mg/mL. It also showed antiperistalsis, antidiarrheal and antisecretory activity in animal models. Thus, C. sativus seeds have therapeutic effects by regulating the contractile response through a calcium-mediated signaling pathway.

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Syzygium cumini(L.),Skeels fruit extracts: In vitro and in vivo anti-inflammatory properties

Cited by 50 publications

References 54 publications

Antioxidant and Anti-Inflammatory Effects of Peganum harmala Extracts: An In Vitro and In Vivo Study

Antioxidant and Anti-Inflammatory Effects of Peganum harmala Extracts: An In Vitro and In Vivo Study

Optimal Extraction Process and In Vivo Anti-Inflammatory Evaluation of High Purity Oily Capsicum Oleoresin for Pharmaceutical Applications

Cucumis sativus L. Seeds Ameliorate Muscular Spasm-Induced Gastrointestinal and Respiratory Disorders by Simultaneously Inhibiting Calcium Mediated Signaling Pathway

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