T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative stress

Wu, Jing; Tu, Di; Liu, Yuan; Yuan, Hui; Wen, Lixin

doi:10.1016/j.etap.2013.03.017

Cited by 63 publications

(41 citation statements)

References 32 publications

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“…[12,28] Activity of SOD and GPx were significantly decreased by T-2 toxin in the rat ovarian granulosa cells. [16] In our study T-2 toxin decreased the activity of SOD, but only slightly at the dose of 100 ng/mL. On the other hand, this mycotoxin significantly increased activity of intracellular GPx in all doses used in this study against the control.…”

Section: Discussioncontrasting

confidence: 62%

“…[34] T-2 toxin significantly decreased the cellular viability in rat ovarian granulosa cells. [16] Our results indicated that T-2 toxin induced ROS accumulation in porcine ovarian granulosa cells, significantly in the group with the highest dose of mycotoxin (100 ng/mL) against all other groups. Treatment with quercetin, expecting for decrease in ROS production, had no effect on elimination of ROS accumulation induced by T-2 toxin.…”

Section: Discussionmentioning

confidence: 62%

“…The question is what would happen if the dose of T-2 toxin would increase. Maybe the cells would be capable to maintain their defence by increasing activities of antioxidant enzymes, or the collapse of the antioxidant defence system would appear by decreasing the enzyme activities, as it was reported by Wu et al [16] Authors observed significant decrease in SOD and GPx activities by T-2 toxin treatment in a dose-dependent manner in the granulosa cells of rats. Overproduction of ROS can be prevented by scavenging enzymes such as SOD and GPx.…”

Section: Discussionmentioning

confidence: 67%

“…Antioxidant (Trolox, a water-soluble vitamin E derivate) significantly inhibited T-2 toxin-induced increase of ROS generation in rat ovarian granulosa cells. [16] In this study, quercetin was effective in maintaining and increasing the TAS level in granulosa cells, also after co-incubation with T-2 toxin. On the contrary, quercetin did not reduce the ROS overproduction caused by T-2 toxin, but had positive effect on activity of SOD.…”

Section: Discussionmentioning

confidence: 94%

“…[13] T-2 toxin has been reported to cause various toxic effects on animals and humans, [13][14][15] e.g. diarrhoea, emesis, haemorrhage, immunosuppression, leukopenia, decreased reproductive capacity, [16] it is a potent embryo toxin. [14] It was reported that T-2 toxin possesses potent cardiac toxicity in rats.…”

Section: Introductionmentioning

confidence: 99%

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Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Capcarová

Petruška

Zbyňovská

et al. 2015

Journal of Environmental Science and Health, Part B

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The aim of this study was to determine the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), production of reactive oxygen species (ROS), and total antioxidant status (TAS) in porcine ovarian granulosa cells after quercetin and T-2 toxin exposure in vitro. Porcine ovarian granulosa cells were incubated with quercetin and T-2 toxin separately or in mutual combination at the doses of 1 ng/mL, 10 ng/mL, 100 ng/mL, and the control group without any additions for 24 h. In this study T-2 toxin developed stress reaction in porcine ovarian granulosa cells and increased generation of ROS. Quercetin had no effect in elimination of ROS generation induced by T-2 toxin, but was effective in maintaining and increasing of TAS, activities of SOD and GPx in porcine granulosa cells in vitro. These results contribute towards the understanding of cellular stress and its response.

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Section: Discussioncontrasting

confidence: 62%

Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Capcarová

Petruška

Zbyňovská

et al. 2015

Journal of Environmental Science and Health, Part B

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Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

Nikoloff

Pascua

Anchordoquy

et al. 2017

Cell Biology International

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The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitro maturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1 μM, and 1 mM EPA. The effect of EPA was evaluated on cumulus-oocyte complexes (COC) by oocyte maturation (cumulus expansion area and oocyte nuclear maturation), genotoxicity [single cell gel electrophoresis (SCGE)], and cytotoxicity (apoptosis, viability, and MTT assays) end points. The maturation parameters were affected by exposure of COC to different EPA concentrations in the IVM medium. Cumulus expansion area increased in the presence of 1 nM EPA (P < 0.05) whereas addition of 1 nM EPA (P < 0.05) decreased cumulus expansion after 24 h of IVM. Moreover, the maturation rate significantly decreased when 1 mM of EPA was assayed (P < 0.001). EPA at 1 nM induced genotoxic and cytotoxic effects on bovine cumulus cells (CC) and primary DNA lesions (P < 0.001). A significant increase in the frequency of apoptotic (P < 0.01) and necrotic (P < 0.001) cells was observed after 24 h of treatment with 1 nM, 1 μM, and 1 mM EPA. Mitochondrial activity was altered with 1 mM EPA (P < 0.001). We inferred that optimal oocyte quality was partially dependent on the presence of adequate EPA concentrations; EPA could be beneficial to improve oocyte quality in the maturation process, because low concentration tested (1 nM EPA) improved cumulus expansion.

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Effects of Alginic Acid on the Porcine Granulosa Cells and Maturation of Porcine Oocytes

Wang,

Li,

Yuan

et al. 2023

Molecular Nutrition Food Res

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ScopeAlginic acid (AA) from brown algae is a marine organic compound. There is extensive use of AA in the food industry and healthcare, suggesting a high probability of AA exposure. The present study investigates the effects of AA on porcine ovarian granulosa cells (GCs) and oocytes to explore its mechanism in female reproduction because of its adverse effects on reproduction.Methods and resultsThe study adds 20 µM AA to the porcine primary ovarian GCs medium and porcine oocyte in vitro maturation (IVM) medium. Estrogen and progesterone levels are downregulated in GCs. Reactive oxygen species are excessive, and the antioxidant capacity declines. Then mitochondria‐mediated apoptosis pathway is involved in GCs apoptosis. In addition, scores of autophagosomes are found in the experimental cells. Furthermore, AA significantly inhibits the proliferation of GCs around cumulus‐oocyte complexes (COCs) accompanied by abnormal spindle assembly, chromosome arrangement disorder, and aberrant cortical granules distribution in oocytes, leading to a decreased oocyte maturation rate.ConclusionThese findings suggest that 20 µM AA is toxic to sow reproduction by interfering with estrogen production, oxidative stress, mitochondria‐mediated apoptosis, autophagy in GCs of sows, and oocyte maturation.

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T-2 toxin exposure induces apoptosis in rat ovarian granulosa cells through oxidative stress

Cited by 63 publications

References 32 publications

Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Changes in antioxidant status of porcine ovarian granulosa cells after quercetin and T-2 toxin treatment

Effect of eicosapentaenoic acid on bovine cumulus–oocyte complex in vitro

Effects of Alginic Acid on the Porcine Granulosa Cells and Maturation of Porcine Oocytes

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