T‐cell activation during exacerbations: a longitudinal study in refractory asthma

Mamessier, Émilie; Nieves, Ana; Lorec, Anne-Marie; Dupuy, P; Pinot, D.; Pinet, C.; Vervloët, D.; Magnan, A.

doi:10.1111/j.1398-9995.2008.01687.x

Cited by 70 publications

(48 citation statements)

References 26 publications

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“…There are already numerous reports demonstrating the presence of lymphocytes with specific subset markers (Mamessier et al 2008;Dominguez Ortega et al 2004;Lourenco et al 2009). Antibodies for the other leukocyte populations were selected based on the well known surface antigen expression of peripheral blood leukocytes.…”

Section: Discussionmentioning

confidence: 93%

Flow cytometry analysis of leukocytes in induced sputum from asthmatic patients

Vidal

Bellido-Casado²,

Granel³

et al. 2012

Immunobiology

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Section: Discussionmentioning

confidence: 93%

Flow cytometry analysis of leukocytes in induced sputum from asthmatic patients

Vidal

Bellido-Casado²,

Granel³

et al. 2012

Immunobiology

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“…Importantly, in two latter studies, steroid-naive and steroid-treated patients were analyzed jointly. Most recently, Mamessier et al found decreased levels of CD4+CD25+CD62L+CD45RO+lymphocytes in patients with severe refractory asthma [16]. In fact, it is difficult to compare results from above studies as they come from patients of different age, with varying levels of severity of asthma and different treatment regimens.…”

Section: Discussionmentioning

confidence: 97%

Effects of Oral Glucocorticoid Therapy on CD4+CD25+CD127- and CD4+CD25high T Cell Levels in Asthmatic Patients

2010

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Our purpose was to evaluate the effects of short-term oral glucocorticoid (GC) treatment on frequencies of T cells with putative regulatory phenotype (namely, CD4+CD25+CD127- and CD4+CD25high) in patients with asthma exacerbations. In addition, we sought to determine frequencies of above T cell subsets in adult asthmatic patients in relation to disease severity and different treatment regimens. The analysis was performed in 62 patients with different stages of asthma and ten healthy controls. Polychromatic flow cytometry was applied to delineate T cells with CD4+CD25+CD127- and CD4+CD25high phenotype. Exhaled nitric oxide analysis was used to assess allergic airway inflammation. Levels of neither CD4+CD25+CD127- nor CD4+CD25high T cells were significantly altered after 7-day oral GC treatment. Importantly, there were no detectable differences in frequencies of those cells among studied groups of asthmatics with different severity of disease and healthy controls. Moreover, levels of CD4+CD25+CD127- and CD4+CD25high T cells in asthmatic patients were not correlated to exhaled nitric oxide concentrations. Our data indicate that neither effects of average doses of oral GC treatment nor disease severity are related to changes in frequencies of CD4+CD25+CD127- and CD4+CD25high T cells in adult asthmatic patients.

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“…Sché matiquement, l'inflammation à é osinophiles est en rapport avec une ré ponse immunitaire de type Th2 tandis que l'inflammation à neutrophiles correspond à une ré ponse Th1 (figure 2). Dans le travail rapporté ci-dessus, une activation mixte Th1 et Th2 é tait trouvé e au niveau des lymphocytes T dans le sang et l'expectoration induite [20], avec une diminution des lymphocytes T ré gulateurs. Il est donc probable que l'hé té rogé né ité de l'inflammation au cours des exacerbations ne soit pas uniquement dé pendante du type d'activation lymphocytaire T, et mette en jeu des mé canismes variables en fonction des causes.…”

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