T-cell clonality of undetermined significance

Dippel, Edgar; Klemke, Detlev; Hummel, Michael; Stein, Harald; Goerdt, Sergij

doi:10.1182/blood.v98.1.247

Cited by 42 publications

(36 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As no identical T cell clones were identified in both the skin and the peripheral blood, the detection of T-cell monoclonality in the peripheral blood of 21% of the patients should be interpreted with caution, i.e. as T-cell clonality of undetermined significance [37]. In summary, the immunophenotypic and molecular findings presented here support the classification of JLIS as a CD8+ polyclonal reactive skin condition.…”

Section: Commentsupporting

confidence: 56%

Jessner’s Lymphocytic Infiltration of the Skin: A CD8+ Polyclonal Reactive Skin Condition

et al. 2003

View full text Add to dashboard Cite

Background: Jessner’s lymphocytic infiltration of the skin (JLIS) is a clinically and histologically distinct disease entity. Conflicting results have been reported concerning its differentiation from cutaneous lupus erythematosus and polymorphous light eruption, its relationship to palpable migratory arciform erythema and its classification as a B-cell or a CD4+ T-cell lymphoproliferative disease. Objective: Our study was performed in order to re-evaluate JLIS clinically and by immunohistochemical and molecular analyses. Methods: Stringent inclusion/exclusion criteria were used to collect a cohort of 34 patients with JLIS that did not overlap with lupus erythematosus or polymorphous light eruption. Clinical data were analysed, and immunohistochemical and molecular studies were performed including TCR-γ PCR GeneScan software analysis of tissue and peripheral blood samples. Results: In the majority of the patients, the lesions consisted only of papules and plaques while in 12% annular lesions were also seen. The lesions were found on the face (38%), on the trunk and arms (50%) or at both sites (12%). Immunohistochemical analyses revealed a clear predominance of T cells in all patients, and of CD8+ T cells in 77% of the patients. As judged by TCR-γ PCR GeneScan analysis, 98 and 79% of the tissue and peripheral blood samples, respectively, showed a polyclonal T-cell population; identical T-cell clones were not detected concomitantly in both the skin and the peripheral blood of the same patient. Conclusions: JLIS occurs at 2 major predilection sites, that is the face and trunk. Therefore introduction of palpable migratory arciform erythema as a separate entity is not justified. The lymphoid infiltrates are dominated immunohistochemically by CD8+ T cells that do not show clonality on molecular analysis. Thus, JLIS represents a characteristic CD8+ polyclonal reactive skin condition.

show abstract

Section: Commentsupporting

confidence: 56%

Jessner’s Lymphocytic Infiltration of the Skin: A CD8+ Polyclonal Reactive Skin Condition

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Unfortunately, T-cell receptor gene rearrangement analyses do not consistently distinguish between malignant and benign cases when the peripheral blood is analysed. We have termed this phenomenon T-cell clonality of undetermined significance [40]. Therefore, the diagnosis of leukaemic involvement in SS is solely based on the cytological determination of Sézary cells in blood smears and on FACS analysis for loss of CD7 or – more reliably – for absence of CD26 expression [41].…”

Section: Commentmentioning

confidence: 99%

Overexpression of c-<i>myb</i> in Leukaemic and Non-Leukaemic Variants of Cutaneous T-Cell Lymphoma

et al. 2005

View full text Add to dashboard Cite

Background: The c-myb oncogene is a transcription factor that regulates proliferation, differentiation and apoptosis of haematopoietic cells and activated T cells by binding to promoter sequences of such genes as c-myc or bcl-2 that are expressed in cutaneous T-cell lymphoma (CTCL). Objective: Our study was performed in order to evaluate c-myb expression as a quantitative parameter for differential diagnosis in leukaemic and non-leukaemic variants of CTCL. Methods: c-myb expression was analysed in lesional skin and in the peripheral blood of 21 patients with mycosis fungoides (MF), 15 patients with Sézary syndrome (SS) and 15 patients with inflammatory skin diseases using immunohistochemistry and semiquantitative as well as quantitative RT-PCR. Results: Immunohistochemistry confirmed expression of c-myb in the lesional skin of the majority of CTCL patients with a tendency towards higher expression in SS (1.86 ± 0.5) versus MF (1.2 ± 0.7) while c-myb was absent from the lesional skin of patients with inflammatory skin diseases. c-myb was overexpressed in the peripheral blood in all SS patients (100% SS vs. 35.7% MF) at a high expression level (51,335.31 ± 31,960.32 AU in SS vs. 1,226.35 ± 1,258.29 AU in MF using semiquantitative RT-PCR, and 5.72 × 10^–2 ± 2.27 × 10^–2 in SS vs. 0.91 × 10^–2 ± 1.18 × 10^–2 in MF vs. 0.24 × 10^–2 ± 0.11 × 10^–2 in inflammatory skin disease using quantitative RT-PCR). CD4+ cells from the peripheral blood of SS patients and cell lines in vitro showed the highest c-myb expression levels upon quantitative RT-PCR (23.27 × 10^–2 and 10.78 × 10^–2 ± 7.24 × 10^–2). Conclusion: Overexpression of c-myb in skin lesions of both non-leukaemic and leukaemic CTCL independent of the stage of the disease indicates that it acts early in disease development. Nevertheless, if positive, c-myb expression in lesional skin is a clear-cut diagnostic marker for CTCL as compared to inflammatory skin diseases. High-level expression of c-myb in the peripheral blood as assessed by quantitative RT-PCR constitutes an additional diagnostic parameter for SS and may be especially useful in cases in which morphological determination of Sézary cells or FACS analysis of CD7 and CD26 remain inconclusive.

show abstract

“…2 Sezary cells are malignant circulating CD4 þ T cells that derive from the same T-cell clone as the malignant T cells in skin and other organs as demonstrated by T-cell receptor (TCR) gene rearrangement analysis studies. 3,4 The detection of Sezary cells in the peripheral blood is primarily based on morphological features such as cerebriform nuclei; therefore, 'Sezary cells' may be detected in healthy individuals as well as in inflammatory dermatoses. As a consequence, an arbitrary threshold has been set at 5% (20%) Sezary cells among peripheral blood mononuclear cells (peripheral blood lymphocytes) for the diagnosis of SS in the blood.…”

Section: Introductionmentioning

confidence: 99%

Sézary syndrome is a unique cutaneous T-cell lymphoma as identified by an expanded gene signature including diagnostic marker molecules CDO1 and DNM3

et al. 2007

View full text Add to dashboard Cite

T-cell clonality of undetermined significance

Cited by 42 publications

References 7 publications

Jessner’s Lymphocytic Infiltration of the Skin: A CD8+ Polyclonal Reactive Skin Condition

Jessner’s Lymphocytic Infiltration of the Skin: A CD8+ Polyclonal Reactive Skin Condition

Overexpression of c-<i>myb</i> in Leukaemic and Non-Leukaemic Variants of Cutaneous T-Cell Lymphoma

Sézary syndrome is a unique cutaneous T-cell lymphoma as identified by an expanded gene signature including diagnostic marker molecules CDO1 and DNM3

Contact Info

Product

Resources

About