T-cell cytokine responses in human infection with Mycobacterium tuberculosis

Zhang, Ming; Lin, Yu‐Pin; Iyer, Dinakar; Gong, Jianhua; Abrams, John S.; Barnes, Peter F.

doi:10.1128/iai.63.8.3231-3234.1995

Cited by 258 publications

(108 citation statements)

References 21 publications

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“…When analysed for secretion of disease-associated type 2 cytokine IL-10, PBMC of healthy subjects secreted IL-10 in response to whole-cell M. tuberculosis and MT-CW but not in response to MT-CF. Similar findings were reported by Zhang et al, who demonstrated secretion of IL-10 by PBMC in response to whole-cell M. tuberculosis (Zhang et al, 1995). Because IL-10 downregulates the protective type 1 response (Turner et al, 2002), the antigens selected to design a vaccine against TB should not induce the secretion of IL-10.…”

Section: Discussionsupporting

confidence: 86%

“…In summary, although several studies have been carried out in the past to investigate the cytokine profiles in TB patients and healthy subjects using sera and culture supernatants from PBMC and T-cell clones (Barnes et al, 1993;Zhang et al, 1995;Lin et al, 1996;Lai et al, 1997;Verbon et al, 1999), only two of these studies have followed protocols somewhat similar to ours and reported results that are consistent with our findings (Zhang et al, 1995;Lin et al, 1996). In these studies, however, only whole-cell M. tuberculosis was used as antigen to study the profiles of IFNg and IL-10 secreted by PBMC of TB patients and healthy subjects (Zhang et al, 1995;Lin et al, 1996). We, on the other hand, have studied the cytokine profiles in response to whole-cell M. tuberculosis and a wide range of other complex and single antigens of M. tuberculosis.…”

Section: Discussionmentioning

confidence: 99%

“…A number of studies have been performed to determine cytokine profiles in the sera of TB patients (Taha et al, 1997;Verbon et al, 1999) and supernatants of antigen nonstimulated peripheral blood mononuclear cells (PBMC) (Demissie et al, 2004;Salina & Morozova, 2004;Bal et al, 2005) as well as PBMC stimulated with whole-cell M. tuberculosis (Zhang et al, 1995;Lin et al, 1996) and mitogens (Bal et al, 2005). However, knowledge of the cytokine profile of human PBMC in response to various complex and single antigens of M. tuberculosis is limited.…”

Section: Introductionmentioning

confidence: 99%

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Cytokine profiles in tuberculosis patients and healthy subjects in response to complex and single antigens ofMycobacterium tuberculosis

Al‐Attiyah

Madi

El-Shamy

et al. 2006

FEMS Immunology &Amp; Medical Microbiology

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Peripheral blood mononuclear cells (PBMC) were obtained from tuberculosis (TB) patients and Mycobacterium bovis bacillus Calmette-Guerin vaccinated healthy subjects. PBMC were tested for secretion of tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-5 (IL-5) and IL-10 in response to complex (whole cells, culture filtrate and cell walls), single secreted (Ag85B, ESAT6, MPT64, PstS and MPT70) and single cytosolic (DnaK, GroES and GroEL) antigens of Mycobacterium tuberculosis. In the absence of antigens, detectable concentrations of TNF-alpha, IFN-gamma and IL-10 were secreted by PBMC of both donor groups, but the concentrations of only IL-10 were significantly higher (P=0.015) in TB patients than in healthy subjects. In the presence of complex antigens, PBMC secreted IFN-gamma and TNF-alpha in response to all three preparations, whereas IL-10 was secreted in response to whole cells and cell walls only. In the presence of single antigens, IFN-gamma was secreted in response to Ag85B, ESAT6 and MPT64 in TB patients and ESAT6 in healthy donors. Except for GroEL and DnaK, single antigens did not induce TNF-alpha and IL-10 secretion from PBMC in either donor group. The secretion of IFN-gamma, but not IL-10, in the presence of Ag85B, ESAT6 and MPT64 supports their potential as subunit vaccine candidates against TB.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytokine profiles in tuberculosis patients and healthy subjects in response to complex and single antigens ofMycobacterium tuberculosis

Al‐Attiyah

Madi

El-Shamy

et al. 2006

FEMS Immunology &Amp; Medical Microbiology

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“…During the course of successful treatment of tuberculosis patients, IgE levels appear to drop signi®cantly (Adams et al 1999). Although there is overwhelming evidence that tuberculosis itself causes transient depression of Th1 cell function, and transient enhanced Th2 phenotype cell function, some con¯icting results as regards Th2 enhancement have been reported (Zhang et al 1995) but these differences might be explained by stage and severity of tuberculosis (Dlugovitzky et al 1997) or other confounding factors that in¯uence Th1/2 balance, such as age (Shearer 1997) and nutritional state (Dai et al 1998). In tuberculoid leprosy, Th1-like cells predominate with a resistant immune response, whereas Th2-like cells are dominant in lepromatous leprosy patients with ineffective immunity (Yamamura et al 1991;Chensue et al 1994Chensue et al , 1997.…”

Section: Mycobacterial Disease Resulting From Immune Impairment Inducmentioning

confidence: 99%

Susceptibility to development of Mycobacterium ulcerans disease: review of possible risk factors

Stienstra

Graaf

Meerman

et al. 2001

Tropical Med Int Health

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SummaryMycobacterium ulcerans disease, also known as Buruli ulcer (BU), is a disease of subcutaneous fat tissue. BU is prevalent in riverine and swamp areas of the tropical zone in Africa, Asia and South America, and a few scattered foci in Australia. The mode of transmission of M. ulcerans has not been fully elucidated, but inoculation into the subcutaneous tissues probably occurs through penetrating skin trauma. BU has not been linked with HIV infection. Antimycobacterial drug treatment is ineffective, and treatment is surgical. Patients eventually develop scars and contractures, with resulting disabilities, and the disease imposes a large burden on affected populations. The incidence of BU has dramatically increased in West African countries over the last decade. There is an urgent need for research into host and environmental risk factors for BU in order to develop effective strategies to combat this disease. We review possible genetic host susceptibility factors for BU that are relevant in other mycobacterial diseases: natural resistance-associated macrophage protein-1 (NRAMP-1), HLA-DR, vitamin D 3 receptor, mannose binding protein, interferon-gamma (IFN-c) receptor, tumour necrosis factor alpha (TNF-a), interleukin (IL)-1a, 1b and their receptor antagonists; and IL-12. Schistosoma haematobium infection is highly endemic in many BU foci in West Africa, with a striking increase in transmission after river dams were constructed. This observation, and the observations from interaction of schistosomiasis and tuberculosis, have fuelled our hypothesis that schistosomiasis is a risk factor for BU by driving the host immune response towards a predominantly Th-2 pattern, away from a Th-1 preponderant protection against mycobacterial infection. If the latter hypothesis is con®rmed, enhanced schistosomiasis control should impact on BU.

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“…14 A number of studies have shown that levels of IFN-g production are much lower in TB patients than in healthy controls. [15][16][17][18] Mycobacteria-infected macrophages also release large quantities of IL-6. Levels are elevated in bronchoalveolar lavage fluid of patients with active TB, and it is thought that it may play a role in multinucleated giant cell (MGC) formation from alveolar macrophages.…”

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confidence: 99%

An in vitro model of the leukocyte interactions associated with granuloma formation in Mycobacterium tuberculosis infection

et al. 2007

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The principal defense of the human host against a Mycobacterium tuberculosis infection is the formation of granulomas, organized collections of activated macrophages, including epithelioid and multinucleated giant cells, surrounded by lymphocytes. This granuloma can sequester and contain the bacteria preventing active disease, and if the granuloma is maintained, these bacteria may remain latent for a person's lifetime. Secretion of a variety of chemoattractant cytokines following phagocytosis of the bacilli by the macrophage is critical not only to the formation of the granuloma but also to its maintenance. To investigate this process of early granuloma formation, we developed an in vitro model composed entirely of human cells. Combining blood lymphocytes and autologous macrophages from healthy purified protein derivative skin testnegative individuals and mycobacteria resulted in the formation of small, rounded aggregate structures. Microscopic examination found macrophage-specific CD68 + epithelioid macrophages and small round CD3 + lymphocytes that in complex resembled small granulomas seen in clinical pathology specimens. Acid-fast staining bacteria were observed between and possibly within the cells composing the granulomas. Supernatants from the infected cells collected at 24 and 48 h and 5 and 9 days after infection were analyzed by a multiplexed cytokine bead-based assay using the Luminex 100 and were found to contain interleukin (IL)-6, IL-8, interferon-c and tumor necrosis factor-a, cytokines known to be involved in human granuloma formation, in quantities from two-fold to 7000-fold higher than supernatants from uninfected control cells. In addition, chemotaxis assays demonstrated that the same supernatants attracted significantly more human peripheral blood mononuclear cells than those of uninfected cells (Po0.001). This model may provide insight into the earliest stages of granuloma formation in those newly infected.

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T-cell cytokine responses in human infection with Mycobacterium tuberculosis

Cited by 258 publications

References 21 publications

Cytokine profiles in tuberculosis patients and healthy subjects in response to complex and single antigens ofMycobacterium tuberculosis

Cytokine profiles in tuberculosis patients and healthy subjects in response to complex and single antigens ofMycobacterium tuberculosis

Susceptibility to development of Mycobacterium ulcerans disease: review of possible risk factors

An in vitro model of the leukocyte interactions associated with granuloma formation in Mycobacterium tuberculosis infection

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