T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Tordesillas, Leticia; Cuesta‐Herranz, Javier; González‐Muñoz, Miguel; Pacios, Luis F.; Compés, E.; Garcia-Carrasco, Belen; Sánchez‐Monge, Rosa; Salcedo, Gabriel; Díaz‐Perales, Araceli

doi:10.1016/j.molimm.2008.10.018

Cited by 50 publications

(45 citation statements)

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“…The regions of Pru p 3 bound by IgE antibodies have been previously identified [13], and recombinant Pru p 3 has been produced [14]. However, few primary data have been published that identify T-cell epitopes of Pru p 3 [15,16]. …”

Section: Introductionmentioning

confidence: 99%

Characterization of the T-Cell Epitopes of the Major Peach Allergen Pru p 3

Pastorello

Monza

Pravettoni

et al. 2010

Int Arch Allergy Immunol

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Background: Pru p 3 is the major peach allergen recognized by more than 90% of peach-allergic individuals of the Mediterranean area. Identification of the dominant Pru p 3 T-cell epitopes can improve our understanding of the immune responses against this protein and could be helpful in the development of hypoallergenic immunotherapy. For this purpose, we examined the phenotypes, specificities and cytokine secretion profiles of proliferating T cells in response to Pru p 3 in peach-allergic individuals. Methods: Peripheral blood mononuclear cells from 15 peach-allergic patients were incubated with Pru p 3. The proliferation of antigen-specific T-cell lines (TCLs) was assessed by tritiated methylthymidine incorporation. T-cell epitopes were identified by analyzing the reactivity of TCLs against 8 overlapping peptides spanning the entire length of Pru p 3. We characterized the phenotype of Pru-p-3-specific TCLs by flow cytometry and analyzed their production of interleukin (IL) 4 and γ-interferon (IFN-γ) by ELISA. Results: Ninety-two Pru-p-3-specific TCLs were isolated (stimulation index ≧5). These TCLs proliferated mainly in response to Pru p 3_12–27 and Pru p 3_57–72. Pru-p-3-specific TCLs were mainly CD4+ (81%) and expressed cell surface CD30. In addition, TCLs produced high levels of IL-4 and low levels of IFN-γ, indicating a Th2 phenotype. Conclusions: Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 3_12–27 and Pru p 3_57–72. These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.

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Section: Introductionmentioning

confidence: 99%

Characterization of the T-Cell Epitopes of the Major Peach Allergen Pru p 3

Pastorello

Monza

Pravettoni

et al. 2010

Int Arch Allergy Immunol

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“…In contrast, different Pru p 3-epitopes have been characterized by several methods Pacios et al, 2008;Tordesillas et al, 2009). Thus, three sequential regions, Pru p 3n-25.…”

Section: Introductionmentioning

confidence: 99%

Molecular basis of allergen cross-reactivity: Non-specific lipid transfer proteins from wheat flour and peach fruit as models

Tordesillas¹,

Pacios²,

Palacín³

et al. 2009

Molecular Immunology

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“…For example, peripheral blood mononuclear cells (PBMCs) from 18 peach allergic patients and peach allergen (Pru p 3) specific T cell lines from nine patients were cultured and analyzed for proliferation response in the presence of 17 overlapping peptides that spans the entire sequence of Pru p 3 [41]. Each peptide is of 10-amino acid residues long with five overlapping amino acids.…”

Section: Mapping Of T Cell Epitopesmentioning

confidence: 99%

“…T cells epitopes have been mapped in a number of food allergens including the cow's milk allergen αs1-casein [53], peanut allergen Ara h 2 [54], peach allergen Pru p 3 [41], egg allergen ovalbumin (OVA) [42] and egg-white allergen ovomucoid (Ovm) [55]. However, investigations on the feasibility of these epitopes being utilized in PIT and the possible clinical outcome were restricted to egg allergy to date.…”

Section: Applications Of T Cell Epitopes In Therapymentioning

confidence: 99%

From Molecule Studies of Allergens to Development of Immunotherapy of Allergies

Wai¹,

Leung²,

Chu³

et al. 2012

J Aller Ther

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IntroductionAllergy is a type I hypersensitivity disease prominently influences the quality of life. Allergens can strike one through the administration of drugs, insect sting, and ingestion of food or simply inhalation, making patients hard to strictly avoid these molecules. Allergen specific immunotherapy for grass pollen allergy has been put into clinical trials since 1950s and demonstrated satisfactory therapeutic effects [1]. It therefore paves the way for extensive researches on designing different allergen specific immunotherapy and unveiling the underlying mechanisms of these therapies.Defining the immunological mechanisms leading to allergy is crucial in designing allergen-specific immunotherapy. Sensitization of allergic individuals to allergens is initialized upon presentation of the allergen-derived peptides by the antigen presenting cells (APCs) through the major histocompatibility class II molecule (MHC class II) to naïve T cells, which are then activated and differentiated into type 2 T helper cells (Th2) (Figure 1). Cytokines such as IL-4 and IL-13 from Th2 cause a class switch in B cells to produce IgE antibodies specific to the particular allergen. When the individual is exposed to the same allergen again, IgE are massively produced, cross-link with the allergen and high affinity receptor (FcεRI) on mast cells and induce degranulation. Mediators such as histamine and leukotriene are released, resulting in inflammation and other associated anaphylactic responses [2].Molecular characteristics of allergens, in particular the identification of T cell and B cell epitopes, constitute important information for the design of therapeutic regimens. T cell epitopes refer to the short peptide fragments activating Th2 via MHC class II molecules while B cell epitopes, or IgE-binding epitopes, refer to the regions recognized by IgE on the allergen. With the identification of B cell epitopes at the molecular level, hypo-allergens possessing reduced allergenicity can be constructed through the introduction of point mutations on these IgE-binding regions [3,4]. The lack of IgE reactivity reduces the risk of these hypo-allergens to form cross-links with IgE and thus prevents the development of allergic side effects during treatment [5]. Meanwhile, since the T cell epitopes are conserved, these hypo-allergens are immunogenic and thus offer therapeutic effects. Alternatively, the employment of T cell epitopes in immunotherapy is believed to be a potential strategy since small peptide fragments are incapable to form cross-link with IgE but yet immunogenic to modulate immune responses [6]. AbstractAllergies are hypersensitive reactions that affect over 25% of the world population. Extensive studies have been directed to define patho-immunological mechanisms of allergy and the molecular characteristics of allergens. The emerging B cell and T cell epitope database has greatly facilitated the development of epitope-based immunotherapy that aims at modulating patients' immune responses towards a specific allergen. Modifi...

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T-cell epitopes of the major peach allergen, Pru p 3: Identification and differential T-cell response of peach-allergic and non-allergic subjects

Cited by 50 publications

References 33 publications

Characterization of the T-Cell Epitopes of the Major Peach Allergen Pru p 3

Characterization of the T-Cell Epitopes of the Major Peach Allergen Pru p 3

Molecular basis of allergen cross-reactivity: Non-specific lipid transfer proteins from wheat flour and peach fruit as models

From Molecule Studies of Allergens to Development of Immunotherapy of Allergies

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