T-cell growth factor (Interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines

Abstract

Mononuclear blood cells from patients with different types of leukemia, and from controls as well as cells from established lymphoblastic cell lines were analyzed with respect to terminal transferase (TdT) activity and T-cell growth factor (TCGF; Interleukin 2, IL-2), to determine the significance of TCGF production and response as functional markers for human leukemias. The data obtained so far suggest that the aberrant proliferation and lack of maturation observed in these leukemias may be associated with or be the result of a break-down in cellular-mediated control of proliferation.

Targeting CD6 for the treatment of experimental autoimmune uveitis

“…After blocking with 1% BSA in PBS for 1 h, the plate was incubated with cell culture supernatants at RT for 2 h. After washing, the plate was incubated with HRP-lableled anti-mouse IgG at RT for 1h, followed by color development by adding the HRP substrate TMB and OD 450 measurement using a microplate reader (Molecular Devices, San Jose, CA,USA). Clones with positive ELISA results were subsequently expanded, and the IgGs were purified and subjected to flow cytometric analyses of binding to CD6 on T cells from WT and CD6 KO mice and a human T cell line, Molt-4 [22].…”

Darstellung der terminalen Deoxynukleotidyl-Transferase im histologischen Schnitt*

“…Vermutlich aufgrund der engen Korrelation der Anwesenheit von Glukokortikoidrezeptoren an der Zellmembran und TdT in den Zellen läßt sich ein gutes Ansprechen der TdT-positiven lymphatischen Leukämien auf Kombinationen von Vincristin und Prednisolon erklären (10). TdT-negative lymphatische Leukämien und TdT-positive nicht-lymphatische Leukämien bedeuten sowohl im Erwachsenenalter als auch im Kindesalter eine relativ schlechtere Prognose (10,11,18). Basierend auf biochemisch-enzymatischen Untersuchungen einer beträchtlichen Fa11zahl von akuten Leukämien des Kindes haben kürzlich Weite und Mitarb.…”