T Cell Mapping of One Epitope from Thyroglobulin Inducing Experimental Autoimmune Thyroiditis (EAT)

Abstract

These data collect the advance made in the last few years in our laboratory in defining one epitope from the thyroglobulin (Tg) molecule (660 KDa) inducing Experimental Autoimmune thyroiditis (EAT) in CBA/J mice. We achieved the characterization of one EAT-inducer Tg peptide by combining "in vitro" biochemical and immunological approaches and "in vivo" studies. Since T cells recognize degraded forms of the antigen and since endogenous antigens preferentially activate class I-restricted T cells, we hypothesized that one cytotoxic T cell hybridoma, named HTC2, which prevents further EAT induction in mice injected with Tg would be specific for one EAT inducer peptide. In order to identify one Tg epitope inducing EAT, enzymatic treatment of the protein by trypsin, HPLC purification and sequence analysis were performed. Simultaneously, tryptic digests were used to pulse CBA/J macrophages and tested for their ability to be recognized by HTC2 cells. Lastly, when digests were recognized by HTC2 cells their capacity to induce EAT in CBA/J mice was evaluated. To further assess the pathogenicity of the sequenced Tg peptide, one synthetic peptide was made and its capacity to induce EAT verified. By this procedure we identified for the first time one 40 amino-acid peptide from human thyroglobulin inducing EAT in CBA/J mice.

Effect of intrathymic administration of mycobacterial heat shock protein 65 and peptide p277 on the development of diabetes in NOD mice: caution required in vaccination studies

“…On the other hand, although the phenomenon of "T-cell suppression" has been the subject of considerable controversy in recent years, several data suggest its involvement in animal diabetes. First, autoreactive T cells are present at the periphery, since many autoimmune diseases can be experimentally induced by antigen immunization with adjuvant (21,22). Second, i.t.…”

Relation of anti‐TPO autoantibody titre and T‐lymphocyte cytokine production patterns in Hashimoto's thyroiditis

“…Thyroid peroxidase (TPO) is a sequestered antigen that does not normally interact with the immune system, but does when released following damage to thyroid tissue. TPO‐specific T cells were frequently identified in the thyroid of HT patients 10 . Several overlapping B‐cell epitopes were identified.…”

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