T-cell mitogens cause early changes in cytoplasmic free Ca2+ and membrane potential in lymphocytes

Tsien, Roger Y.; Pozzan, Tullio; Rink, T J

doi:10.1038/295068a0

Cited by 1,129 publications

(615 citation statements)

References 28 publications

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“…Mitogenic stimulation complete, but it has been shown that there is a rapid increase in [Ca'+]i in lymphocytes within 1 min of the addition of mitogens [4,5], with no detectable increase in pHi within 60 min [6]. In contrast, in fibroblasts there is an early increase in pHi in response to mitogens that appears to be driven by Na+/H+ exchange [7,8] but no corresponding measurements of [Ca'+]r over the same period have been reported.…”

Section: Growth Factormentioning

confidence: 99%

Some mitogens cause rapid increases in free calcium in fibroblasts

et al. 1984

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Quiescent 3T3 fibroblasts grown on microcarrier beads and loaded with the [Ca"] indicator quin2 had a cytosolic free Ca'+ concentration ([Ca*']i) of 154 + 11 nM (SE; n = 32). Stimulation with the mitogens vasopressin, epidermal growth factor (EGF) or prostaglandin Fh (PGFh) caused a very rapid increase in [Ca*+]r to a maximum of 200-5OOnM after 60-90s. [Ca*+]i declined thereafter to a level above that in quiescent cells which was maintained for at least 15 min. In contrast no immediate effects on [Ca*+]r were detected after the addition of the mitogens insulin or 12-O-tetradecanoylphorbol 13-acetate (TPA). These studies indicate that early changes in [Ca*']r may be involved in the action on fibroblasts of some, but not all, mitogens.Free Ca2' Quin2 3T3 fibroblast

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Section: Growth Factormentioning

confidence: 99%

Some mitogens cause rapid increases in free calcium in fibroblasts

et al. 1984

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“…The cyclic AMP analogue N6,02'-dibutyryladenosine 3 ' ,5 '-cyclic monophosphate (DB cyclic AMP) blocks growth initiation by serum and other mitogens and Ca*+ influx into cells [8, 29,30,61,62]. This raises the question whether the inhibition of growth by cyclic AMP is due solely to its ability to prevent excess calcium from entering cells at a critical growth restriction point [12] in Gl phase of the cell cycle.…”

Section: Cyclic Amp and Ca*+ Availabilitymentioning

confidence: 99%

“…Some evidence that this could be true exists. Thus concanavalin A activation of small lymphocytes requires Ca*+ and the influx of Ca*+ into concanavalin A-treated cells is inhibited by DB cyclic AMP, as is DNA synthesis [29]. Furthermore either DB cyclic AMP or EGTA prevents PY815 mouse mastocytoma cells from receiving a flux of Ca*+ required for growth [59] and either reagent inhibits the influx of Ca*+ and subsequent DNA synthesis upon restoration of serum to serum-deprived 3T3 cells [30].…”

Section: Cyclic Amp and Ca*+ Availabilitymentioning

confidence: 99%

Cyclic AMP, calcium and control of cell growth

Ralph

1983

FEBS Letters

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The role of cyclic AMP and calcium in the control of normal and tumour cell growth is considered in relation to the question whether cyclic AMP is a true mitogen or cc-mitogen. It is proposed that cyclic AMP normally controls the cell cycle at a point in Gl phase only by virtue of its ability to exclude calcium required by cells to progress past this point into S phase. Therefore increased influx of calcium by other routes induced by various factors can bypass the inhibitory effect of cyclic AMP and stimulate growth. In these circumstances cyclic AMP or calcium may or may not facilitate further progress into S phase according to the metabolic requirements of individual cells. The relevance to cancer cells is considered. Cyclic AMP Calcium

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“…Tsien et al, 1982). Although other intracellular probes are available (see, e.g., Borle and Snowdowne, 1986), the fluorescent indicators quin2 and more recently fura2 have been used by most researchers to measure intracellular free Ca2+ in hepatocytes, largely on account of their ease of handling and non-invasive properties.…”

Section: Introductionmentioning

confidence: 99%