T-cryptantigen (TCA) activation in surgical neonates: a hidden problem

Grant, Hugh W.; Hadley, G. P.; Adhikari, M.; Fernandes-Costa, F.

doi:10.1007/s003830050702

Cited by 2 publications

(4 citation statements)

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“…Rather, non-adherent growth was removed and fresh medium was added at 6, 11 and 14 hr following initiation of the experiment. At each of these time-points, and at 17 hr, some cultures were terminated and the growth of adherent bacteria was quanti®ed by 14 C incorporation. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Tubes were washed three times with saline and the tubes were emptied of all liquid before the photograph was taken. Representative tubes are shown in (a), and the amount of adherent growth as quanti®ed by 14 Cincorporation is shown in (b). The experiment was reproducible.…”

Section: Discussionmentioning

confidence: 99%

“…13 Further, natural antibodies are known to bind to autologous cells following removal of their cell surface carbohydrates. 14,15 Thus, it might be postulated that sIgA actually facilitates the adhesion of bacterial cells to epithelial cells under certain conditions. Removal of cell surface carbohydrate by bacteria was assessed using succ-WGA, a lectin that recognizes a structure (GlcNAcb1±4GlcNAc) that is exposed following removal of terminal saccharides.…”

Section: Increased Binding Of Siga To Human Epithelial Cells Followinmentioning

confidence: 99%

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Human secretory immunoglobulin A may contribute to biofilm formation in the gut

Bollinger

Everett²,

Palestrant³

et al. 2003

Immunology

171

127

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It is critical, both for the host and for the long-term benefit of the bacteria that colonize the gut, that bacterial overgrowth with subsequent bacterial translocation, which may lead to sepsis and death of the host, be avoided. Secretory IgA (sIgA) is known to be a key factor in this process, agglutinating bacteria and preventing their translocation in a process termed 'immune exclusion'. To determine whether human sIgA might facilitate the growth of normal enteric bacteria under some conditions, the growth of human enteric bacteria on cultured, fixed human epithelial cells was evaluated in the presence of sIgA or various other proteins. Human sIgA was found to facilitate biofilm formation by normal human gut flora and by Escherichia coli on cultured human epithelial cell surfaces under conditions in which non-adherent bacteria were repeatedly washed away. In addition, the presence of sIgA resulted in a 64% increase in adherence of E. coli to live cultured epithelial cells over a 45-min period. Mucin, another defence factor thought to play a key role in immune exclusion, was found to facilitate biofilm formation by E. coli. Our findings suggest that sIgA may contribute to biofilm formation in the gut.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Increased Binding Of Siga To Human Epithelial Cells Followinmentioning

confidence: 99%

See 1 more Smart Citation

Human secretory immunoglobulin A may contribute to biofilm formation in the gut

Bollinger

Everett²,

Palestrant³

et al. 2003

Immunology

171

127

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“…21 There have been several studies on the role of different inflammatory cytokines in the pathogene-SY Chan and KL Chan sis of NEC. [21][22][23][24][25][26] Currently, the clinical effect of their measurement and monitoring is still under investigation.…”

Section: Hypoxic-ischaemic Insultsmentioning

confidence: 99%

Pathogenesis of neonatal necrotizing enterocolitis

Chan

2001

Annals of the College of Surgeons of Hong Kong

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With recent advances in the medical management of premature infants, a higher proportion survive. Subsequently, necrotizing enterocolitis (NEC) has evolved in the past 30 years and is currently the most common gastrointestinal emergency in the neonatal intensive care unit. Necrotizing enterocolitis is associated with a high morbidity and mortality. Predominant suggested risk factors are prematurity, enteral feeding, infectious agents and intestinal hypoxia and ischaemic insult. Intestines of premature infants are defensively immature structurally and functionally. Recent studies on breast‐milk protection of NEC showed that oligosaccharide and glycoconjugates of the breast milk can alter bowel flora to lactobacillus so as to prevent enteropathogen growth. Prebiotics have been shown to promote bifidobacteria and subsequently prevention of NEC. Probiotic bifidobacteria supplementation also shows a similar beneficial effect. The use of prebiotics or probiotics to prevent NEC warrants further studies.

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T-cryptantigen (TCA) activation in surgical neonates: a hidden problem

Cited by 2 publications

References 0 publications

Human secretory immunoglobulin A may contribute to biofilm formation in the gut

Human secretory immunoglobulin A may contribute to biofilm formation in the gut

Pathogenesis of neonatal necrotizing enterocolitis

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