T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives

Jones, Jonathan D. G.; Gilbert, David E.; Grady, Karen L.; Jorgensen, Richard A.

doi:10.1007/bf00331618

Cited by 148 publications

(98 citation statements)

References 10 publications

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“…We are continuing this analysis using inverse PCR to clone out sequences which flank the T-DNA inserts. This should reveal whether incomplete T-DNA transfer is more common in A. fhaliana than in other species where this problem has been or is being investigated (Gheysen eta/., 1987;Jones eta/., 1987;Jorgensen et a/., 1987).…”

Section: Discussionmentioning

confidence: 99%

Behaviour of the maize transposable element Ac in Arabidopsis thaliana

Dean¹,

Sjödin

Page³

et al. 1992

The Plant Journal

102

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SummaryThe somatic and germinal activity of the maize transposable element, Ac, has been analysed in progeny of 43 transformants of A. thaliana using a streptomycin resistance assay to monitor Ac excision. The ability to assay somatic activity enabled, for the first time, a detailed analysis of Ac activity in individual A. thaliana seedlings to be made. The effects of T-DNA copy number, generation, dosage at each locus, flanking sequences and orientation of the element were compared. The most striking observation was the variability in Acactivity in genotypically identical individuals and the poor penetrance of the variegated phenotype. In general, increasing Ac dosage increased both somatic and germinal excision frequencies. The majority of families from individuals selected as inheriting an excision event carried transposed Ac elements re-integrated in different positions in the genome.

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Section: Discussionmentioning

confidence: 99%

Behaviour of the maize transposable element Ac in Arabidopsis thaliana

Dean¹,

Sjödin

Page³

et al. 1992

The Plant Journal

102

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“…An important factor of gene silence is generally known for the integrated location of transgene and the multi-copy per integration site (Stam et al 1997). In special, expression of the transgene may be activated if becoming integrated into euchromatin of chromosome (Koncz et al 1989), whereas inactivated if inserted into repetitive DNA or heterochromatin of chromosome (Pröls and Meyer 1992), and also often show low expression in case of multi-copy (Jones et al 1987). Like these, non-expression of scEDIII gene in the seven plants may be speculated by gene silencing mechanism, except for the plant of V662-29-3864.…”

Section: Generation Of Transgenic Maize Plantsmentioning

confidence: 99%

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Kim¹,

Kwon²,

Yang³

et al. 2014

Journal of Plant Biotechnology

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Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two-and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

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“…Moreover, methylation of the foreign DNA following stable integration into the genome can effect its expression [89, 901. Copy number of the insert does not generally appear to account for the variation in levels of expression observed [91], nor can it necessarily be overcome by nesting the gene construct of interest between flanking sequences of up to 10 kb in size [92]. Position effects will cause difficulties when attempting to correlate differing levels of gene expression with different gene constructs, for example in promoter analysis.…”

Section: Analysis Of Transgenic Plantsmentioning

confidence: 99%

Techniques in plant molecular biology – progress and problems

Walden

Schell

1990

European Journal of Biochemistry

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Progress in plant molecular biology has been dependent on efficient methods of introducing foreign DNA into plant cells. Gene transfer into plant cells can be achieved by either direct uptake of DNA or the natural process of gene transfer carried out by the soil bacterium Agrobacterium. Versatile gene‐transfer vectors have been developed for use with Agrobacterium and more recently vectors based on the genomes of plant viruses have become available. Using this technology the expression of foreign DNA, the functional analysis of plant DNA sequences, the investigation of the mechanism of viral DNA replication and cell to cell spread, as well as the study of transposition, can be carried out. In addition, the versatility of the gene‐transfer vectors is such that they may be used to isolate genes not amenable to isolation using conventional protocols. This review concentrates on these aspects of plant molecular biology and discusses the limitations of the experimental systems that are currently available.

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T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives

Cited by 148 publications

References 10 publications

Behaviour of the maize transposable element Ac in Arabidopsis thaliana

Behaviour of the maize transposable element Ac in Arabidopsis thaliana

Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

Techniques in plant molecular biology – progress and problems

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