T lymphoblastoid cell lines from Marek's disease lymphomas

Powell, P. C.; Payne, L. N.; Frazier, Judith A.; Rennie, M.

doi:10.1038/251079a0

Cited by 166 publications

(77 citation statements)

References 4 publications

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“…Ten chicken cell lines established from chicken lymphoma were used in this study, MD cell lines: MSB1 [1], MTB1 [9], JP1, JP2 [31], HP1, HP2 [20] and RP1 [18]; AL cell lines: 1104B1 [6] and CU10 [3] an RE cell line: Ku7 [22]. All cell lines were grown at 40°C in RPMI1640 medium supplemented with 10% fetal bovine serum, penicillin G (200 U/ml), streptomycin (200 mg/ml) and 2 × 10 5 M 2-mercaptoethanol.…”

Section: Cell Lines and Tumor Samplesmentioning

confidence: 99%

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

Takagi

Ohashi

Morimura

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. To determine whether there is any abnormalities of the p53 gene in chicken lymphoblastoid tumor cell lines derived from Marek's disease (MD), lymphoid leukosis, reticuloendotheliosis, and field tumors, some portions of p53 cDNA corresponding to core and C-terminal domains (nucleotide positions 277-1104 in the p53 open reading frame (ORF)) were sequenced. Several mutations were identified in both cell lines and field tumors. However, none of these mutations is localized at the "hot spot", which has been reported as the site for transformation-activating mutations. Moreover, partial cDNA clones with a 122-bp deletion in the p53 ORF were identified in two cell lines, MSB1 and MTB1 derived from MD tumors. Southern blot analysis showed that no deletion occurred in the genome of p53 in MSB1, indicating that deletion occurred at the transcriptional level. This deletion could cause a frame shift of the encoding p53 protein, possibly resulting in the generation of a functionally different p53 protein. However, we confirmed that p53 mRNA without deletion is also present in each of these cell lines. These mutations of the p53 gene and deletion in the p53 transcript may be ones of molecular changes specific to the transformation induced by MD virus. -KEY WORDS: Marek's disease, p53, RT-PCR, transformation.J. Vet. Med. Sci. 60(8): 923-929, 1998 demyelination of peripheral nerves [23]. The molecular mechanism of transformation by MDV has not been fully understood. Unlike retroviruses, MDV does not have any apparent oncogenes, suggesting that interaction of viral proteins with cellular oncoproteins or activation of cellular oncogenes are important for the transformation mechanism. Recently, it has been reported that Meq, which has a homology with members of the jun/fos oncoprotein family, could be a viral oncogene based on its potential role as a transcription factor [30]. In addition, the Meq protein has been shown to have a potential to interact with p53 [2], suggesting possible involvement of this interaction in the transformation process by MDV. However, no information is available concerning the changes in the p53 gene of host cells related to transformation by MDV. Thus, to determine whether p53 mutations are involved in the neoplastic process of chicken lymphoma, we analyzed the molecular structure of chicken p53 genes and its transcripts in chicken tumor cell lines derived from MD, avian leukosis (AL), reticuloendotheliosis (RE), and field tumors. We here reported that tumor cell lines possess mis-sense mutations and deletion in the p53 transcript. MATERIALS AND METHODS Cell

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Section: Cell Lines and Tumor Samplesmentioning

confidence: 99%

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

Takagi

Ohashi

Morimura

et al. 1998

The Journal of Veterinary Medical Science

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“…This is in contrast to Burkitt's lymphoma, which shows many similarities to MD, and from which cell lines were relatively easy to establish Epstein, 1970). In the present communication, the fine structure of two cell lines (Powell et al, 1974) (Fig. 2, 3).…”

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confidence: 57%

“…The MD-derived HPRS cell lines are also of thymic origin (Powell et al, 1974). This identification is supported by the ultrastructural finding of abundant ribosomes and sparse endoplasmic reticulum in the cells.…”

Section: )1d( U Ssionmentioning

confidence: 73%

“…Whilst the desirability of establishing MD lymphoma-derived cell lines has been realized for some time (Klein, 1972), it is only recently that such cell lines have become available (Akiyama, Kato and Iwa, 1973;Akiyama and Kato, 1974;Powell et al, 1974). This is in contrast to Burkitt's lymphoma, which shows many similarities to MD, and from which cell lines were relatively easy to establish Epstein, 1970).…”

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confidence: 99%

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The ultrastructure of lymphoblastoid cell lines from Marek's disease lymphomata

Frazier¹,

Powell²

1975

Br J Cancer

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Summary.-The ultrastructure of two lymphoblastoid cell lines derived from Marek's disease lymphomata has been studied. The cells varied from 5 to 12 ,um in diameter and had large round or oval nuclei. A nucleolus was occasionally present and about 3%0 of cells showed projections of the nuclear envelope. The cytoplasm contained many ribosomes and several mitochondria but endoplasmic reticulum was sparse. A small number of cells contained annulate lamellae and crystalline structures were occasionally seen. Cells with immature intranuclear herpesvirus particles were rarely present. The cells had many ultrastructural features in common with Burkitt's lymphoma-derived cell lines.

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“…Since an MD lymphoma-derived lymphoblastoid cell line was first established by Akiyama et al (1973), a number of cell lines have been established. AU of these cultured cells have been shown to carry both a T-ceU marker (Nazerian & Sharma, 1975;Matsuda et al, 1976a;Calnek et al, 1978) and MD tumour-associated surface antigen (MATSA) (Powell et al, 1974;Witter et al, 1975;Matsuda et al, 1976b). On the other hand, cell lines derived from lymphomas of lymphoid leukosis (LL) caused by avian leukosis virus (ALV) have been shown to carry a B-cell marker (Matsuda et al, 1976a) and have been used as a negative control of MATSA.…”

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confidence: 99%

Expression of Forssman Antigen of Avian Lymphoblastoid Cell Lines Transformed by Marek's Disease Virus or Avian Leukosis Virus

Ikuta

Kitamoto

Shoji

et al. 1981

Journal of General Virology

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SUMMARYThe expression of Forssman-type heterophile antigen on Marek's disease (MD) virus (MDV)-transformed cell lines, MDCC-MSB 1, -HP 1, -RP 1 and -BP 1, and avian leukosis virus (ALV)-transformed cell lines, LSCC-1104B1 and -1104X5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. Forssman antigen was detected on a high percentage of the cells in two ALV-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. Of the MDV-transformed cell lines tested only the RP1 and BP1 cell lines, derived from transplantable MD tumours, expressed Forssman antigen, while the MSB 1 and HP 1 cell lines, derived from MD lymphomas, did not. Forssman antigen appears to be unrelated to MD tumour-associated surface antigen (MATSA).

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T lymphoblastoid cell lines from Marek's disease lymphomas

Cited by 166 publications

References 4 publications

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

The ultrastructure of lymphoblastoid cell lines from Marek's disease lymphomata

Expression of Forssman Antigen of Avian Lymphoblastoid Cell Lines Transformed by Marek's Disease Virus or Avian Leukosis Virus

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